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GH_EBVB9
ID   GH_EBVB9                Reviewed;         706 AA.
AC   P03231; Q6LBU6; Q777C0;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 113.
DE   RecName: Full=Envelope glycoprotein H {ECO:0000255|HAMAP-Rule:MF_04033};
DE            Short=gH {ECO:0000255|HAMAP-Rule:MF_04033};
DE   Flags: Precursor;
GN   Name=gH {ECO:0000255|HAMAP-Rule:MF_04033}; ORFNames=BXLF2;
OS   Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Gammaherpesvirinae; Lymphocryptovirus.
OX   NCBI_TaxID=10377;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=6087149; DOI=10.1038/310207a0;
RA   Baer R., Bankier A.T., Biggin M.D., Deininger P.L., Farrell P.J.,
RA   Gibson T.J., Hatfull G., Hudson G.S., Satchwell S.C., Seguin C.,
RA   Tuffnell P.S., Barrell B.G.;
RT   "DNA sequence and expression of the B95-8 Epstein-Barr virus genome.";
RL   Nature 310:207-211(1984).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-116.
RX   PubMed=6092825;
RA   Bankier A.T., Deininger P.L., Farrell P.J., Barrell B.G.;
RT   "Sequence analysis of the 17,166 base-pair EcoRI fragment C of B95-8
RT   Epstein-Barr virus.";
RL   Mol. Biol. Med. 1:21-45(1983).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 338-389.
RX   PubMed=2835748; DOI=10.1093/nar/16.7.2859;
RA   Walls D., Perricaudet M., Gannon F.;
RT   "The analysis of EBV proteins which are antigenic in vivo.";
RL   Nucleic Acids Res. 16:2859-2872(1988).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=2831372; DOI=10.1128/jvi.62.4.1101-1107.1988;
RA   Heineman T., Gong M., Sample J., Kieff E.;
RT   "Identification of the Epstein-Barr virus gp85 gene.";
RL   J. Virol. 62:1101-1107(1988).
RN   [5]
RP   INTERACTION WITH GP25 AND GP42.
RX   PubMed=9621012; DOI=10.1128/jvi.72.7.5552-5558.1998;
RA   Wang X., Kenyon W.J., Li Q., Mullberg J., Hutt-Fletcher L.M.;
RT   "Epstein-Barr virus uses different complexes of glycoproteins gH and gL to
RT   infect B lymphocytes and epithelial cells.";
RL   J. Virol. 72:5552-5558(1998).
RN   [6]
RP   FUNCTION.
RX   PubMed=11021994; DOI=10.1006/viro.2000.0531;
RA   Oda T., Imai S., Chiba S., Takada K.;
RT   "Epstein-Barr virus lacking glycoprotein gp85 cannot infect B cells and
RT   epithelial cells.";
RL   Virology 276:52-58(2000).
RN   [7]
RP   SUBCELLULAR LOCATION.
RX   PubMed=15534216; DOI=10.1073/pnas.0407320101;
RA   Johannsen E., Luftig M., Chase M.R., Weicksel S., Cahir-McFarland E.,
RA   Illanes D., Sarracino D., Kieff E.;
RT   "Proteins of purified Epstein-Barr virus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:16286-16291(2004).
RN   [8]
RP   FUNCTION, AND INTERACTION WITH HOST EPHA2.
RX   PubMed=29292383; DOI=10.1038/s41564-017-0080-8;
RA   Zhang H., Li Y., Wang H.B., Zhang A., Chen M.L., Fang Z.X., Dong X.D.,
RA   Li S.B., Du Y., Xiong D., He J.Y., Li M.Z., Liu Y.M., Zhou A.J., Zhong Q.,
RA   Zeng Y.X., Kieff E., Zhang Z., Gewurz B.E., Zhao B., Zeng M.S.;
RT   "Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus
RT   entry.";
RL   Nat. Microbiol. 3:1-8(2018).
RN   [9]
RP   FUNCTION, AND INTERACTION WITH HOST EPHA2.
RX   PubMed=29292384; DOI=10.1038/s41564-017-0081-7;
RA   Chen J., Sathiyamoorthy K., Zhang X., Schaller S., Perez White B.E.,
RA   Jardetzky T.S., Longnecker R.;
RT   "Ephrin receptor A2 is a functional entry receptor for Epstein-Barr
RT   virus.";
RL   Nat. Microbiol. 3:172-180(2018).
RN   [10]
RP   X-RAY CRYSTALLOGRAPHY (3.58 ANGSTROMS) OF 20-672, DISULFIDE BOND, AND
RP   GLYCOSYLATION AT ASN-60; ASN-435; ASN-549; ASN-604 AND ASN-664.
RX   PubMed=21149717; DOI=10.1073/pnas.1011806108;
RA   Matsuura H., Kirschner A.N., Longnecker R., Jardetzky T.S.;
RT   "Crystal structure of the Epstein-Barr virus (EBV) glycoprotein
RT   H/glycoprotein L (gH/gL) complex.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:22641-22646(2010).
RN   [11]
RP   X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 20-674, GLYCOSYLATION AT ASN-60,
RP   AND INTERACTION WITH GLYCOPROTEIN 42/BZLF2.
RX   PubMed=27929061; DOI=10.1038/ncomms13557;
RA   Sathiyamoorthy K., Hu Y.X., Moehl B.S., Chen J., Longnecker R.,
RA   Jardetzky T.S.;
RT   "Structural basis for Epstein-Barr virus host cell tropism mediated by gp42
RT   and gHgL entry glycoproteins.";
RL   Nat. Commun. 7:13557-13557(2016).
RN   [12]
RP   X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 20-679.
RX   PubMed=28939750; DOI=10.1073/pnas.1704661114;
RA   Sathiyamoorthy K., Jiang J., Moehl B.S., Chen J., Zhou Z.H., Longnecker R.,
RA   Jardetzky T.S.;
RT   "Inhibition of EBV-mediated membrane fusion by anti-gHgL antibodies.";
RL   Proc. Natl. Acad. Sci. U.S.A. 114:703-710(2017).
CC   -!- FUNCTION: The heterodimer glycoprotein H-glycoprotein L is required for
CC       the fusion of viral and plasma membranes leading to virus entry into
CC       the host cell. Following initial binding to host receptor, membrane
CC       fusion is mediated by the fusion machinery composed of gB and the
CC       heterodimer gH/gL. May also be involved in the fusion between the
CC       virion envelope and the outer nuclear membrane during virion
CC       morphogenesis. The heterodimer gH/gL targets also host EPHA2 to promote
CC       viral entry. {ECO:0000255|HAMAP-Rule:MF_04033,
CC       ECO:0000269|PubMed:11021994, ECO:0000269|PubMed:29292383,
CC       ECO:0000269|PubMed:29292384}.
CC   -!- SUBUNIT: Interacts with glycoprotein L (gL); this interaction is
CC       necessary for the correct processing and cell surface expression of gH.
CC       The heterodimer gH/gL seems to interact with gB trimers during fusion.
CC       The heterodimer gH/gL interacts with host EPHA2 to facilitate virus
CC       internalization and fusion (PubMed:29292384). Interacts with
CC       glycoprotein 42/BZLF2 (PubMed:27929061). {ECO:0000255|HAMAP-
CC       Rule:MF_04033, ECO:0000269|PubMed:27929061,
CC       ECO:0000269|PubMed:29292384}.
CC   -!- INTERACTION:
CC       P03231; P03212: gL; NbExp=3; IntAct=EBI-15815779, EBI-15897767;
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000255|HAMAP-
CC       Rule:MF_04033}; Single-pass type I membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_04033}. Host cell membrane {ECO:0000255|HAMAP-Rule:MF_04033};
CC       Single-pass type I membrane protein {ECO:0000255|HAMAP-Rule:MF_04033}.
CC       Host endosome membrane {ECO:0000255|HAMAP-Rule:MF_04033}; Single-pass
CC       type I membrane protein {ECO:0000255|HAMAP-Rule:MF_04033}. Note=During
CC       virion morphogenesis, this protein probably accumulates in the
CC       endosomes and trans-Golgi where secondary envelopment occurs. It is
CC       probably transported to the cell surface from where it is endocytosed
CC       and directed to the trans-Golgi network (TGN). {ECO:0000255|HAMAP-
CC       Rule:MF_04033}.
CC   -!- PTM: N-glycosylated, O-glycosylated, and sialylated.
CC       {ECO:0000255|HAMAP-Rule:MF_04033}.
CC   -!- SIMILARITY: Belongs to the herpesviridae glycoprotein H family.
CC       {ECO:0000255|HAMAP-Rule:MF_04033}.
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DR   EMBL; V01555; CAA24797.1; -; Genomic_DNA.
DR   EMBL; AJ507799; CAD53450.1; -; Genomic_DNA.
DR   EMBL; X07532; CAE82041.1; -; Genomic_DNA.
DR   PIR; A93065; QQBE6L.
DR   PIR; S00736; S00736.
DR   RefSeq; YP_401700.1; NC_007605.1.
DR   PDB; 3PHF; X-ray; 3.58 A; 1/3/5/A/C/E/G/I/K/M/O/Q/S/U/W/Y=20-672.
DR   PDB; 5T1D; X-ray; 3.10 A; A=20-674.
DR   PDB; 5W0K; X-ray; 3.10 A; A/C=20-679.
DR   PDB; 7CZE; X-ray; 3.00 A; A/C/E/G=18-674.
DR   PDBsum; 3PHF; -.
DR   PDBsum; 5T1D; -.
DR   PDBsum; 5W0K; -.
DR   PDBsum; 7CZE; -.
DR   SMR; P03231; -.
DR   DIP; DIP-49016N; -.
DR   IntAct; P03231; 2.
DR   iPTMnet; P03231; -.
DR   PRIDE; P03231; -.
DR   ABCD; P03231; 3 sequenced antibodies.
DR   DNASU; 3783742; -.
DR   GeneID; 3783742; -.
DR   KEGG; vg:3783742; -.
DR   SIGNOR; P03231; -.
DR   EvolutionaryTrace; P03231; -.
DR   Proteomes; UP000153037; Genome.
DR   GO; GO:0044175; C:host cell endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-UniRule.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019064; P:fusion of virus membrane with host plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.40.3190; -; 1.
DR   HAMAP; MF_04033; HSV_GH; 1.
DR   InterPro; IPR003493; Herpes_gH.
DR   InterPro; IPR035305; Herpes_glycoH_C.
DR   InterPro; IPR038172; Herpes_glycoH_C_sf.
DR   Pfam; PF17488; Herpes_glycoH_C; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Disulfide bond;
KW   Fusion of virus membrane with host cell membrane;
KW   Fusion of virus membrane with host membrane; Glycoprotein;
KW   Host cell membrane; Host endosome; Host membrane; Membrane;
KW   Reference proteome; Sialic acid; Signal; Transmembrane;
KW   Transmembrane helix; Viral envelope protein;
KW   Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04033"
FT   CHAIN           19..706
FT                   /note="Envelope glycoprotein H"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04033"
FT                   /id="PRO_0000436649"
FT   TOPO_DOM        19..682
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04033"
FT   TRANSMEM        683..703
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04033"
FT   TOPO_DOM        704..706
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04033"
FT   REGION          165..229
FT                   /note="Interaction with gL"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04033"
FT   CARBOHYD        60
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04033,
FT                   ECO:0000269|PubMed:21149717"
FT   CARBOHYD        435
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04033,
FT                   ECO:0000269|PubMed:21149717"
FT   CARBOHYD        549
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04033,
FT                   ECO:0000269|PubMed:21149717"
FT   CARBOHYD        604
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04033,
FT                   ECO:0000269|PubMed:21149717"
FT   CARBOHYD        664
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04033,
FT                   ECO:0000269|PubMed:21149717"
FT   DISULFID        120..312
FT                   /evidence="ECO:0000269|PubMed:21149717"
FT   DISULFID        278..335
FT                   /evidence="ECO:0000269|PubMed:21149717"
FT   DISULFID        454..478
FT                   /evidence="ECO:0000269|PubMed:21149717"
FT   DISULFID        534..587
FT                   /evidence="ECO:0000269|PubMed:21149717"
FT   DISULFID        612..615
FT                   /evidence="ECO:0000269|PubMed:21149717"
FT   CONFLICT        380..389
FT                   /note="TVGYPKAGVY -> GAPGGRAGPP (in Ref. 3; CAE82041)"
FT                   /evidence="ECO:0000305"
FT   STRAND          22..29
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          32..39
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           40..46
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           52..58
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   TURN            59..62
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           65..72
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          100..102
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          104..106
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          115..117
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          119..122
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           123..130
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          135..138
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   TURN            142..147
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          151..154
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          156..162
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   TURN            163..166
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          167..173
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          175..185
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          187..189
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          191..200
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           201..203
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           213..216
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          217..221
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          224..231
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           232..239
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           246..255
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           258..274
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   TURN            275..279
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           285..306
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          310..314
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           315..335
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          339..342
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           346..356
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           361..363
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           371..381
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           383..385
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           392..405
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           415..432
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          434..436
FT                   /evidence="ECO:0007829|PDB:5T1D"
FT   HELIX           440..453
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           456..468
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           471..473
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           477..480
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          481..483
FT                   /evidence="ECO:0007829|PDB:5T1D"
FT   HELIX           488..492
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          499..501
FT                   /evidence="ECO:0007829|PDB:5T1D"
FT   HELIX           503..515
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   TURN            520..522
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          524..527
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   TURN            530..533
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          540..547
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          550..556
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          572..575
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          578..583
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   TURN            613..616
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          618..623
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   TURN            624..626
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          627..633
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   HELIX           637..645
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   TURN            646..648
FT                   /evidence="ECO:0007829|PDB:5T1D"
FT   STRAND          656..661
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          663..665
FT                   /evidence="ECO:0007829|PDB:7CZE"
FT   STRAND          667..670
FT                   /evidence="ECO:0007829|PDB:7CZE"
SQ   SEQUENCE   706 AA;  78322 MW;  A1006BDA6633B1D9 CRC64;
     MQLLCVFCLV LLWEVGAASL SEVKLHLDIE GHASHYTIPW TELMAKVPGL SPEALWREAN
     VTEDLASMLN RYKLIYKTSG TLGIALAEPV DIPAVSEGSM QVDASKVHPG VISGLNSPAC
     MLSAPLEKQL FYYIGTMLPN TRPHSYVFYQ LRCHLSYVAL SINGDKFQYT GAMTSKFLMG
     TYKRVTEKGD EHVLSLVFGK TKDLPDLRGP FSYPSLTSAQ SGDYSLVIVT TFVHYANFHN
     YFVPNLKDMF SRAVTMTAAS YARYVLQKLV LLEMKGGCRE PELDTETLTT MFEVSVAFFK
     VGHAVGETGN GCVDLRWLAK SFFELTVLKD IIGICYGATV KGMQSYGLER LAAMLMATVK
     MEELGHLTTE KQEYALRLAT VGYPKAGVYS GLIGGATSVL LSAYNRHPLF QPLHTVMRET
     LFIGSHVVLR ELRLNVTTQG PNLALYQLLS TALCSALEIG EVLRGLALGT ESGLFSPCYL
     SLRFDLTRDK LLSMAPQEAT LDQAAVSNAV DGFLGRLSLE REDRDAWHLP AYKCVDRLDK
     VLMIIPLINV TFIISSDREV RGSALYEAST TYLSSSLFLS PVIMNKCSQG AVAGEPRQIP
     KIQNFTRTQK SCIFCGFALL SYDEKEGLET TTYITSQEVQ NSILSSNYFD FDNLHVHYLL
     LTTNGTVMEI AGLYEERAHV VLAIILYFIA FALGIFLVHK IVMFFL
 
 
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