GIG1_ARATH
ID GIG1_ARATH Reviewed; 243 AA.
AC Q9M2R1;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Protein GIGAS CELL1;
DE AltName: Full=Protein ENHANCER OF BON1-2 30;
DE AltName: Full=Protein OMISSION OF SECOND DIVISION1;
DE AltName: Full=Protein UV-B-INSENSITIVE 4-like;
GN Name=GIG1; Synonyms=EBO30, OSD1, UVI4-LIKE; OrderedLocusNames=At3g57860;
GN ORFNames=T10K17.70;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=16623893; DOI=10.1111/j.1365-313x.2006.02696.x;
RA Hase Y., Trung K.H., Matsunaga T., Tanaka A.;
RT "A mutation in the uvi4 gene promotes progression of endo-reduplication and
RT confers increased tolerance towards ultraviolet B light.";
RL Plant J. 46:317-326(2006).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19513101; DOI=10.1371/journal.pbio.1000124;
RA d'Erfurth I., Jolivet S., Froger N., Catrice O., Novatchkova M.,
RA Mercier R.;
RT "Turning meiosis into mitosis.";
RL PLoS Biol. 7:E1000124-E1000124(2009).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH FZR2; FZR3; CDC20.1 AND
RP CDC20.5, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=22167058; DOI=10.1105/tpc.111.092049;
RA Iwata E., Ikeda S., Matsunaga S., Kurata M., Yoshioka Y., Criqui M.-C.,
RA Genschik P., Ito M.;
RT "GIGAS CELL1, a novel negative regulator of the anaphase-promoting
RT complex/cyclosome, is required for proper mitotic progression and cell fate
RT determination in Arabidopsis.";
RL Plant Cell 23:4382-4393(2011).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22899078; DOI=10.4161/psb.21133;
RA Iwata E., Ikeda S., Abe N., Kobayashi A., Kurata M., Matsunaga S.,
RA Yoshioka Y., Criqui M.C., Genschik P., Ito M.;
RT "Roles of GIG1 and UVI4 in genome duplication in Arabidopsis thaliana.";
RL Plant Signal. Behav. 7:1079-1081(2012).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, PHOSPHORYLATION BY CDKA-1, AND INTERACTION
RP WITH FZR1; FZR2; FZR3 AND CDC20.1.
RX PubMed=22844260; DOI=10.1371/journal.pgen.1002865;
RA Cromer L., Heyman J., Touati S., Harashima H., Araou E., Girard C.,
RA Horlow C., Wassmann K., Schnittger A., De Veylder L., Mercier R.;
RT "OSD1 promotes meiotic progression via APC/C inhibition and forms a
RT regulatory network with TDM and CYCA1;2/TAM.";
RL PLoS Genet. 8:E1002865-E1002865(2012).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=23345424; DOI=10.1073/pnas.1217024110;
RA Bao Z., Yang H., Hua J.;
RT "Perturbation of cell cycle regulation triggers plant immune response via
RT activation of disease resistance genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:2407-2412(2013).
RN [10]
RP FUNCTION.
RX PubMed=26272661; DOI=10.1186/s12870-015-0558-6;
RA Singh D.K., Spillane C., Siddiqi I.;
RT "PATRONUS1 is expressed in meiotic prophase I to regulate centromeric
RT cohesion in Arabidopsis and shows synthetic lethality with OSD1.";
RL BMC Plant Biol. 15:201-201(2015).
CC -!- FUNCTION: Negative regulator of the anaphase-promoting
CC complex/cyclosome (APC/C) ubiquitin ligase required for proper mitotic
CC and meiotic progression and cell fate determination. Involved in entry
CC into both meiosis I and meiosis II. Prevents endomitosis by
CC preferentially inhibiting APC/C(CDC20). Required for megagametophyte
CC and endosperm development. Triggers mitotic cyclins (e.g. CYCB1-1 and
CC CYCB1-2) accumulation. Confers immunity to bacterial pathogens (e.g.
CC Pseudomonas syringae pv. tomato DC3000), which is associated with
CC increased expression of disease resistance (R) genes. GIG1 and PANS1
CC are part of a network linking centromere cohesion and cell cycle
CC progression through control of APC/C activity.
CC {ECO:0000269|PubMed:19513101, ECO:0000269|PubMed:22167058,
CC ECO:0000269|PubMed:22844260, ECO:0000269|PubMed:22899078,
CC ECO:0000269|PubMed:23345424, ECO:0000269|PubMed:26272661}.
CC -!- SUBUNIT: Interacts with APC/C activators such as FZR1, FZR2, FZR3,
CC CDC20.1 and CDC20.5. {ECO:0000269|PubMed:22167058,
CC ECO:0000269|PubMed:22844260}.
CC -!- TISSUE SPECIFICITY: Expressed in rapidly dividing tissues such as shoot
CC apical meristem and young leaves. Associated with cell division but
CC also with specific cell types. {ECO:0000269|PubMed:22167058}.
CC -!- DEVELOPMENTAL STAGE: Levels fade progressively in a basipetal fashion
CC as the leaf develops. In the epidermis of cotyledons and leaves,
CC observed in dividing and recently divided stomatal precursor cells,
CC especially in dividing guard mother cells and young guard cells. Also
CC present in asymmetrically dividing meristemoid mother cells and
CC meristemoids. In roots, expressed preferentially in the division zones
CC of root tips. {ECO:0000269|PubMed:16623893,
CC ECO:0000269|PubMed:22167058}.
CC -!- PTM: Phosphorylated by CDKA-1 in complex with CYCA1-2.
CC {ECO:0000269|PubMed:22844260}.
CC -!- DISRUPTION PHENOTYPE: Ectopic endomitosis during somatic cell division
CC leading to gigas cells, large guard cells, and round cells containing
CC large polyploid nuclei in cotyledons. Enhancer of the myb3r4 mutant
CC phenotype. Enhancer of bon1-2 phenotype, leading to dwarf and bushy
CC phenotype with many lateral shoots. Production of diploid gametes by
CC skipping the second meiotic division; male meiosis leads to dyads
CC instead of tetrads, and selfed progeny provides tetraploids (4n) and
CC triploids (3n), but no diploid (2n) plants.
CC {ECO:0000269|PubMed:19513101, ECO:0000269|PubMed:22167058,
CC ECO:0000269|PubMed:22844260, ECO:0000269|PubMed:22899078,
CC ECO:0000269|PubMed:23345424}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL132977; CAB67614.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79710.1; -; Genomic_DNA.
DR EMBL; BT004275; AAO42276.1; -; mRNA.
DR EMBL; BT005570; AAO63990.1; -; mRNA.
DR PIR; T46008; T46008.
DR RefSeq; NP_191345.1; NM_115648.5.
DR AlphaFoldDB; Q9M2R1; -.
DR SMR; Q9M2R1; -.
DR BioGRID; 10270; 16.
DR ELM; Q9M2R1; -.
DR IntAct; Q9M2R1; 15.
DR STRING; 3702.AT3G57860.1; -.
DR PaxDb; Q9M2R1; -.
DR PRIDE; Q9M2R1; -.
DR ProteomicsDB; 221892; -.
DR EnsemblPlants; AT3G57860.1; AT3G57860.1; AT3G57860.
DR GeneID; 824955; -.
DR Gramene; AT3G57860.1; AT3G57860.1; AT3G57860.
DR KEGG; ath:AT3G57860; -.
DR Araport; AT3G57860; -.
DR TAIR; locus:2095823; AT3G57860.
DR eggNOG; ENOG502RZ6Y; Eukaryota.
DR HOGENOM; CLU_078386_0_0_1; -.
DR InParanoid; Q9M2R1; -.
DR OMA; DEPNCVM; -.
DR OrthoDB; 1487532at2759; -.
DR PhylomeDB; Q9M2R1; -.
DR PRO; PR:Q9M2R1; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M2R1; baseline and differential.
DR Genevisible; Q9M2R1; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0007113; P:endomitotic cell cycle; IMP:TAIR.
DR GO; GO:0009960; P:endosperm development; IMP:UniProtKB.
DR GO; GO:0009561; P:megagametogenesis; IMP:UniProtKB.
DR GO; GO:0007127; P:meiosis I; IMP:UniProtKB.
DR GO; GO:0007135; P:meiosis II; IMP:UniProtKB.
DR GO; GO:1904667; P:negative regulation of ubiquitin protein ligase activity; IMP:UniProtKB.
DR GO; GO:1900426; P:positive regulation of defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0040020; P:regulation of meiotic nuclear division; IMP:TAIR.
DR GO; GO:0007088; P:regulation of mitotic nuclear division; IMP:TAIR.
DR InterPro; IPR034590; POLYCHOME/GIG1.
DR PANTHER; PTHR35119; PTHR35119; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Developmental protein; DNA replication; Meiosis;
KW Mitosis; Phosphoprotein; Plant defense; Reference proteome.
FT CHAIN 1..243
FT /note="Protein GIGAS CELL1"
FT /id="PRO_0000423304"
SQ SEQUENCE 243 AA; 26995 MW; C6934A766FD96E99 CRC64;
MPEARDRTER PVDYSTIFAN RRRHGILLDE PDSRLSLIES PVNPDIGSIG GTGGLVRGNF
TTWRPGNGRG GHTPFRLPQG RENMPIVTAR RGRGGGLLPS WYPRTPLRDI THIVRAIERR
RGAGTGGDDG RVIEIPTHRQ VGVLESPVPL SGEHKCSMVT PGPSVGFKRS CPPSTAKVQK
MLLDITKEIA EEEAGFITPE KKLLNSIDKV EKIVMAEIQK LKSTPQAKRE EREKRVRTLM
TMR
