GIMA4_MOUSE
ID GIMA4_MOUSE Reviewed; 328 AA.
AC Q99JY3; D3YTN4;
DT 14-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 08-MAY-2019, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=GTPase IMAP family member 4;
DE AltName: Full=Immunity-associated nucleotide 1 protein {ECO:0000303|PubMed:16569770};
DE Short=IAN-1 {ECO:0000303|PubMed:16569770};
DE AltName: Full=Immunity-associated protein 4;
GN Name=Gimap4; Synonyms=Ian1 {ECO:0000303|PubMed:16509771}, Imap4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, INTERACTION WITH CALM1, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, TISSUE SPECIFICITY, PHOSPHORYLATION, DEVELOPMENTAL STAGE, AND
RP MUTAGENESIS OF 239-ARG--ARG-250.
RX PubMed=16569770; DOI=10.1182/blood-2005-11-4616;
RA Schnell S., Demolliere C., van den Berk P., Jacobs H.;
RT "Gimap4 accelerates T-cell death.";
RL Blood 108:591-599(2006).
RN [4]
RP FUNCTION, INTERACTION WITH BAX, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE,
RP AND TISSUE SPECIFICITY.
RX PubMed=16509771; DOI=10.1371/journal.pbio.0040103;
RA Nitta T., Nasreen M., Seike T., Goji A., Ohigashi I., Miyazaki T., Ohta T.,
RA Kanno M., Takahama Y.;
RT "IAN family critically regulates survival and development of T
RT lymphocytes.";
RL PLoS Biol. 4:593-605(2006).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: During thymocyte development, may play a role in the
CC regulation of apoptosis. {ECO:0000269|PubMed:16509771,
CC ECO:0000269|PubMed:16569770}.
CC -!- SUBUNIT: Interacts (via IQ domain) with calmodulin/CALM1 only in the
CC absence of Ca(2+) (PubMed:16569770). Interacts with BAX, but not with
CC other Bcl-2 family members, including BAD, BAK1, BCL2, BCL2L1/Bcl-xL
CC and BCL2L11/BimEL (PubMed:16509771). {ECO:0000269|PubMed:16509771,
CC ECO:0000269|PubMed:16569770}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:16509771,
CC ECO:0000269|PubMed:16569770}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q99JY3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q99JY3-2; Sequence=VSP_060167, VSP_060168;
CC -!- TISSUE SPECIFICITY: Expressed in thymus (in thymocytes), spleen (in
CC splenocytes), lymph node and lung (PubMed:16509771). Expressed in B-
CC cells and T-cells (at protein level) (PubMed:16569770,
CC PubMed:16509771). {ECO:0000269|PubMed:16509771,
CC ECO:0000269|PubMed:16569770}.
CC -!- DEVELOPMENTAL STAGE: Up-regulated during T cell development, including
CC upon the maturation of CD4/CD8 double-positive to CD4 single-positive
CC thymocytes. {ECO:0000269|PubMed:16509771, ECO:0000269|PubMed:16569770}.
CC -!- PTM: Phosphorylated at very low levels in resting splenocytes. Rapidly
CC and transiently phosphorylated in response to splenocyte activation.
CC Phosphorylation is increased in cells undergoing apoptosis.
CC {ECO:0000269|PubMed:16569770}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. T-cell development,
CC selection and activation in vivo appear to occur normally in knockout
CC mice. {ECO:0000269|PubMed:16569770}.
CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family.
CC IAN subfamily. {ECO:0000305}.
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DR EMBL; AC153894; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC005577; AAH05577.1; -; mRNA.
DR CCDS; CCDS20110.1; -. [Q99JY3-1]
DR CCDS; CCDS57423.1; -. [Q99JY3-2]
DR RefSeq; NP_001230128.1; NM_001243199.1. [Q99JY3-2]
DR RefSeq; NP_778155.2; NM_174990.4. [Q99JY3-1]
DR AlphaFoldDB; Q99JY3; -.
DR SMR; Q99JY3; -.
DR IntAct; Q99JY3; 1.
DR STRING; 10090.ENSMUSP00000087524; -.
DR iPTMnet; Q99JY3; -.
DR PhosphoSitePlus; Q99JY3; -.
DR EPD; Q99JY3; -.
DR jPOST; Q99JY3; -.
DR MaxQB; Q99JY3; -.
DR PaxDb; Q99JY3; -.
DR PeptideAtlas; Q99JY3; -.
DR PRIDE; Q99JY3; -.
DR ProteomicsDB; 271222; -. [Q99JY3-1]
DR ProteomicsDB; 357534; -.
DR Antibodypedia; 18614; 302 antibodies from 28 providers.
DR DNASU; 107526; -.
DR Ensembl; ENSMUST00000067506; ENSMUSP00000068398; ENSMUSG00000054435. [Q99JY3-2]
DR Ensembl; ENSMUST00000090070; ENSMUSP00000087524; ENSMUSG00000054435. [Q99JY3-1]
DR GeneID; 107526; -.
DR KEGG; mmu:107526; -.
DR UCSC; uc009bvi.2; mouse. [Q99JY3-1]
DR CTD; 55303; -.
DR MGI; MGI:1349656; Gimap4.
DR VEuPathDB; HostDB:ENSMUSG00000054435; -.
DR eggNOG; ENOG502R7PE; Eukaryota.
DR GeneTree; ENSGT00940000159317; -.
DR HOGENOM; CLU_010468_3_3_1; -.
DR InParanoid; Q99JY3; -.
DR OMA; CKEIARC; -.
DR OrthoDB; 1092873at2759; -.
DR TreeFam; TF330845; -.
DR BioGRID-ORCS; 107526; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Gimap4; mouse.
DR PRO; PR:Q99JY3; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q99JY3; protein.
DR Bgee; ENSMUSG00000054435; Expressed in peripheral lymph node and 186 other tissues.
DR ExpressionAtlas; Q99JY3; baseline and differential.
DR Genevisible; Q99JY3; MM.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR006703; G_AIG1.
DR InterPro; IPR045058; GIMA/IAN/Toc.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10903; PTHR10903; 1.
DR Pfam; PF04548; AIG1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51720; G_AIG1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; GTP-binding; Nucleotide-binding;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..328
FT /note="GTPase IMAP family member 4"
FT /id="PRO_0000190988"
FT DOMAIN 28..230
FT /note="AIG1-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01057"
FT DOMAIN 233..262
FT /note="IQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 37..44
FT /note="G1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01057"
FT REGION 64..68
FT /note="G2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01057"
FT REGION 85..88
FT /note="G3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01057"
FT REGION 154..157
FT /note="G4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01057"
FT REGION 190..192
FT /note="G5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01057"
FT BINDING 37..45
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q9NUV9"
FT BINDING 58
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q9NUV9"
FT BINDING 155..157
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q9NUV9"
FT BINDING 191
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q9NUV9"
FT VAR_SEQ 213..219
FT /note="VRENGGR -> FLSSRMK (in isoform 2)"
FT /id="VSP_060167"
FT VAR_SEQ 220..328
FT /note="Missing (in isoform 2)"
FT /id="VSP_060168"
FT MUTAGEN 239..250
FT /note="RMQELYREELER->QMQELYQEELEQ: Loss of interaction with
FT CALM1."
FT /evidence="ECO:0000269|PubMed:16569770"
SQ SEQUENCE 328 AA; 38044 MW; 10C2446CD3A2D7A7 CRC64;
MEVQCGGAGF IPESSRSSHE LGNQDQGIPQ LRIVLLGKTG AGKSSTGNSI LGEKVFNSGI
CAKSITKVCE KRVSTWDGKE LVVVDTPGIF DTEVPDADTQ REITRYVALT SPGPHALLLV
VPLGRYTVEE HKATQKILDM FGKQARRFMI LLLTRKDDLE DTDIHEYLEK APKFFQEVMH
EFQNRYCLFN NRASGAEKEE QKMQLLTLVQ SMVRENGGRC FTNKMYESAE CVIQKETLRM
QELYREELER EKARIRREYE EQIKDLRDEL EREIRRARME REFKEREAIF TKNQQNARKE
VENTSMILEL IIKAWEIASF IFNQFMKD
