GIR1_ARATH
ID GIR1_ARATH Reviewed; 122 AA.
AC Q9FNI1; A0A178UDX1;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Protein GL2-INTERACTING REPRESSOR 1 {ECO:0000303|PubMed:28526410};
GN Name=GIR1 {ECO:0000303|PubMed:28526410};
GN OrderedLocusNames=At5g06270 {ECO:0000312|Araport:AT5G06270};
GN ORFNames=MHF15.21 {ECO:0000312|EMBL:BAB08949.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, INTERACTION WITH GL2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=28526410; DOI=10.1016/j.bbrc.2017.05.084;
RA Wu R., Citovsky V.;
RT "Adaptor proteins GIR1 and GIR2. I. Interaction with the repressor GLABRA2
RT and regulation of root hair development.";
RL Biochem. Biophys. Res. Commun. 488:547-553(2017).
RN [6]
RP FUNCTION, INTERACTION WITH TPL, SUBCELLULAR LOCATION, EAR MOTIF, AND
RP MUTAGENESIS OF LEU-8; LEU-10 AND LEU-12.
RX PubMed=28526412; DOI=10.1016/j.bbrc.2017.05.085;
RA Wu R., Citovsky V.;
RT "Adaptor proteins GIR1 and GIR2. II. Interaction with the co-repressor
RT TOPLESS and promotion of histone deacetylation of target chromatin.";
RL Biochem. Biophys. Res. Commun. 488:609-613(2017).
CC -!- FUNCTION: Acts as negative regulator of root hair development
CC redundantly with GIR2 (PubMed:28526410). GIR1 and GIR2 may function as
CC adapter proteins that associate with GL2 and participate in the control
CC of root hair formation (PubMed:28526410). GIR1 and GIR2 may function as
CC adapter proteins that associate with TPL and participate in the
CC repression of root gene expression (PubMed:28526412).
CC {ECO:0000269|PubMed:28526410, ECO:0000269|PubMed:28526412}.
CC -!- SUBUNIT: Interacts with GL2 (PubMed:28526410). Interacts with TPL
CC (PubMed:28526412). {ECO:0000269|PubMed:28526410,
CC ECO:0000269|PubMed:28526412}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:28526410,
CC ECO:0000269|PubMed:28526412}.
CC -!- TISSUE SPECIFICITY: Expressed in root and shoot meristems.
CC {ECO:0000269|PubMed:28526410}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but the double mutant seedlings gir1 and gir2 exhibit
CC excessive root hair formation. {ECO:0000269|PubMed:28526410}.
CC -!- MISCELLANEOUS: Seedlings overexpressing GIR1 exhibit reduced root hair
CC formation. {ECO:0000269|PubMed:28526410}.
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DR EMBL; AB006700; BAB08949.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90996.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70099.1; -; Genomic_DNA.
DR EMBL; BT003828; AAO41880.1; -; mRNA.
DR EMBL; BT005129; AAO50662.1; -; mRNA.
DR EMBL; AY084326; AAM60912.1; -; mRNA.
DR RefSeq; NP_001331734.1; NM_001342874.1.
DR RefSeq; NP_196245.1; NM_120710.3.
DR AlphaFoldDB; Q9FNI1; -.
DR IntAct; Q9FNI1; 1.
DR STRING; 3702.AT5G06270.1; -.
DR PaxDb; Q9FNI1; -.
DR PRIDE; Q9FNI1; -.
DR EnsemblPlants; AT5G06270.1; AT5G06270.1; AT5G06270.
DR EnsemblPlants; AT5G06270.2; AT5G06270.2; AT5G06270.
DR GeneID; 830515; -.
DR Gramene; AT5G06270.1; AT5G06270.1; AT5G06270.
DR Gramene; AT5G06270.2; AT5G06270.2; AT5G06270.
DR KEGG; ath:AT5G06270; -.
DR Araport; AT5G06270; -.
DR TAIR; locus:2164235; AT5G06270.
DR eggNOG; ENOG502S48P; Eukaryota.
DR HOGENOM; CLU_109567_2_1_1; -.
DR InParanoid; Q9FNI1; -.
DR OMA; HENSNTM; -.
DR OrthoDB; 1564121at2759; -.
DR PhylomeDB; Q9FNI1; -.
DR PRO; PR:Q9FNI1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FNI1; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0080147; P:root hair cell development; IGI:TAIR.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..122
FT /note="Protein GL2-INTERACTING REPRESSOR 1"
FT /id="PRO_0000450104"
FT REGION 1..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 7..12
FT /note="EAR"
FT /evidence="ECO:0000305|PubMed:28526412"
FT COMPBIAS 12..62
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 8
FT /note="L->S: Abolishes repressor activity and interaction
FT with TPL; when associated with S-10 and S-12."
FT MUTAGEN 10
FT /note="L->S: Abolishes repressor activity and interaction
FT with TPL; when associated with S-8 and S-12."
FT MUTAGEN 12
FT /note="L->S: Abolishes repressor activity and interaction
FT with TPL; when associated with S-8 and S-10."
SQ SEQUENCE 122 AA; 13309 MW; 3DF8F45796BF0B0D CRC64;
MSRRSPKLEL KLNLSPPTSS QRRMVRSPSR SATTSPTSPP SSCVSSEMNQ DEPSVRYSTS
PETTSMVLVG CPRCLMYVML SEDDPKCPKC KSTVLLDFLH ENATNANANA AAASSGRKTR
RN
