GIS3_ARATH
ID GIS3_ARATH Reviewed; 244 AA.
AC Q9C9H1;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Zinc finger protein GIS3 {ECO:0000305};
DE AltName: Full=Protein GLABROUS INFLORESCENCE STEMS 3 {ECO:0000303|PubMed:25640859};
GN Name=GIS3 {ECO:0000303|PubMed:25640859};
GN OrderedLocusNames=At1g68360 {ECO:0000312|Araport:AT1G68360};
GN ORFNames=T22E19.1 {ECO:0000312|EMBL:AAG52593.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=25640859; DOI=10.1111/nph.13218;
RA Sun L., Zhang A., Zhou Z., Zhao Y., Yan A., Bao S., Yu H., Gan Y.;
RT "GLABROUS INFLORESCENCE STEMS3 (GIS3) regulates trichome initiation and
RT development in Arabidopsis.";
RL New Phytol. 206:220-230(2015).
CC -!- FUNCTION: Probable transcription factor required for the initiation of
CC inflorescence trichomes in response to gibberellin and cytokinin. Acts
CC upstream of GIS, GIS2, ZFP8, and the trichome initiation factors GL1
CC and GL3. Binds the promoter region of GIS and GIS2, which may be direct
CC targets of GIS3. {ECO:0000269|PubMed:25640859}.
CC -!- INTERACTION:
CC Q9C9H1; O80748: B-box domain protein 26; NbExp=3; IntAct=EBI-15194063, EBI-15191535;
CC Q9C9H1; O82277: TCP10; NbExp=3; IntAct=EBI-15194063, EBI-3133327;
CC Q9C9H1; Q93Z00: TCP14; NbExp=3; IntAct=EBI-15194063, EBI-4424563;
CC Q9C9H1; Q9C9L2: TCP15; NbExp=3; IntAct=EBI-15194063, EBI-4426144;
CC Q9C9H1; Q9LT89: TCP19; NbExp=4; IntAct=EBI-15194063, EBI-4426178;
CC Q9C9H1; O64647: TCP9; NbExp=3; IntAct=EBI-15194063, EBI-9838721;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9SLB8}.
CC -!- DISRUPTION PHENOTYPE: Decreased number of trichomes in cauline leaves,
CC lateral branches, sepals and main stems. {ECO:0000269|PubMed:25640859}.
CC -!- MISCELLANEOUS: Plants over-expressing GIS3 have increased trichome
CC densities in sepals, cauline leaves, lateral branches, main
CC inflorescence stems, and have ectopic trichomes on carpels.
CC {ECO:0000269|PubMed:25640859}.
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DR EMBL; AC016447; AAG52593.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34785.1; -; Genomic_DNA.
DR EMBL; BT029451; ABK59680.1; -; mRNA.
DR EMBL; AB493526; BAH30364.1; -; mRNA.
DR PIR; D96707; D96707.
DR RefSeq; NP_177003.1; NM_105507.2.
DR AlphaFoldDB; Q9C9H1; -.
DR IntAct; Q9C9H1; 20.
DR STRING; 3702.AT1G68360.1; -.
DR iPTMnet; Q9C9H1; -.
DR PaxDb; Q9C9H1; -.
DR PRIDE; Q9C9H1; -.
DR ProteomicsDB; 220768; -.
DR EnsemblPlants; AT1G68360.1; AT1G68360.1; AT1G68360.
DR GeneID; 843165; -.
DR Gramene; AT1G68360.1; AT1G68360.1; AT1G68360.
DR KEGG; ath:AT1G68360; -.
DR Araport; AT1G68360; -.
DR TAIR; locus:2199367; AT1G68360.
DR eggNOG; ENOG502QTV9; Eukaryota.
DR HOGENOM; CLU_091153_0_0_1; -.
DR InParanoid; Q9C9H1; -.
DR OMA; REVGPTF; -.
DR OrthoDB; 1532684at2759; -.
DR PhylomeDB; Q9C9H1; -.
DR PRO; PR:Q9C9H1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C9H1; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IPI:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IEP:TAIR.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IMP:TAIR.
DR GO; GO:0019760; P:glucosinolate metabolic process; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0010026; P:trichome differentiation; IBA:GO_Central.
DR GO; GO:0010090; P:trichome morphogenesis; IMP:TAIR.
DR InterPro; IPR044299; GIS3/ZFP5/ZFP6.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR PANTHER; PTHR46353; PTHR46353; 1.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE 1: Evidence at protein level;
KW Cytokinin signaling pathway; Developmental protein; Differentiation;
KW Gibberellin signaling pathway; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..244
FT /note="Zinc finger protein GIS3"
FT /id="PRO_0000438923"
FT ZN_FING 73..95
FT /note="C2H2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 26..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 244 AA; 25600 MW; F869A3CFD94DF0CA CRC64;
MEELDFSSKT TTSRLKLFGF SVDGEEDFSD QSVKTNLSSV SPERGEFPAG SSGRSGGGVR
SRGGGGGGGE RKYECQYCCR EFGNSQALGG HQNAHKKERQ QLKRAQLQAT RNAAANFSNA
GSASQFLRNP IVSAFAPPPH LLSSSAVPQP MGGPWMYLPR VSPSQLHVSH GCVIQDGSGG
AGAGGFSYEY GARDSGFGVV GAQMRHVQAH GPRPSVNGFS REVGTTFDDG LGLDLHLSLA
PAGH
