GIS3_YEAST
ID GIS3_YEAST Reviewed; 502 AA.
AC Q12418; D6VY94; Q7LGX3;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Protein GIS3;
GN Name=GIS3; OrderedLocusNames=YLR094C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP GENE NAME.
RX PubMed=10628841; DOI=10.1007/s004380051121;
RA Balciunas D., Ronne H.;
RT "Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1
RT mig1 srb8/10/11 cells.";
RL Mol. Gen. Genet. 262:589-599(1999).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1055 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U53876; AAB67538.1; -; Genomic_DNA.
DR EMBL; Z73266; CAA97656.1; -; Genomic_DNA.
DR EMBL; Z73267; CAA97658.1; -; Genomic_DNA.
DR EMBL; AY692795; AAT92814.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09410.1; -; Genomic_DNA.
DR PIR; S64928; S64928.
DR RefSeq; NP_013195.1; NM_001181981.1.
DR AlphaFoldDB; Q12418; -.
DR BioGRID; 31367; 67.
DR DIP; DIP-4473N; -.
DR IntAct; Q12418; 4.
DR STRING; 4932.YLR094C; -.
DR iPTMnet; Q12418; -.
DR MaxQB; Q12418; -.
DR PaxDb; Q12418; -.
DR PRIDE; Q12418; -.
DR EnsemblFungi; YLR094C_mRNA; YLR094C; YLR094C.
DR GeneID; 850783; -.
DR KEGG; sce:YLR094C; -.
DR SGD; S000004084; GIS3.
DR VEuPathDB; FungiDB:YLR094C; -.
DR eggNOG; ENOG502SGNS; Eukaryota.
DR HOGENOM; CLU_041586_0_0_1; -.
DR InParanoid; Q12418; -.
DR OMA; MLRPCGK; -.
DR BioCyc; YEAST:G3O-32244-MON; -.
DR PRO; PR:Q12418; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q12418; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0035556; P:intracellular signal transduction; IMP:SGD.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..502
FT /note="Protein GIS3"
FT /id="PRO_0000087497"
SQ SEQUENCE 502 AA; 56357 MW; 62FADCCC7FE59337 CRC64;
MLLDVNTNHT LMHDAHVHEH CLIKSIRDDG ALHSWSDSSK VFYPKSFYAT ATNKKNNKLA
SASMNKTATS NRTVSDEIYF HSTKPQFDGQ GSAERTRTLT KRNSFKRTRI LKARDDSELL
NENRSSLMTP SLSSVMSQVR KTNSAKTLSG ECPIHEGHLT QSIKRKFSEE AQSDCSSLSS
SKLHPLTDDI ADAVDLQTPA IGDEVLAEPV VPKMKIININ DLDLFDDWEV KDLVDIFPPV
YERRPRSSSA LSLVSASSDA KLRPTSVDFQ IIDKKGGKTS RRKSRSKSTT ENMIYENDLV
ELEQWPSASP SPETDGSIAS SELLPNKRIR QKSLNTNFLK LYSIETSCKR KSILPEVEVD
DHLLKQLTYS EIRSLEIKKE PNVSTNDIKL ALITRKKLWS DMVHETRNDL FGDSTPWNLH
FVATTSNTEP SQGRESASEH ATADLKSSLV RVHSDVKPWF NNGGTMLKPC GKLNLGKVTN
KTSAPTREIQ YVVKGWCDSR FL
