GIS4_YEAST
ID GIS4_YEAST Reviewed; 774 AA.
AC Q04233; D6VZG9;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Protein GIS4;
GN Name=GIS4; OrderedLocusNames=YML006C; ORFNames=YM9571.13C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=10628841; DOI=10.1007/s004380051121;
RA Balciunas D., Ronne H.;
RT "Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1
RT mig1 srb8/10/11 cells.";
RL Mol. Gen. Genet. 262:589-599(1999).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-622; SER-707 AND SER-720, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165; SER-304; SER-616 AND
RP SER-622, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: May be involved in the Ras/cAMP signaling pathway.
CC {ECO:0000269|PubMed:10628841}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 222 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z49810; CAA89946.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09893.1; -; Genomic_DNA.
DR PIR; S55113; S55113.
DR RefSeq; NP_013708.1; NM_001182361.1.
DR AlphaFoldDB; Q04233; -.
DR BioGRID; 35164; 144.
DR DIP; DIP-1334N; -.
DR IntAct; Q04233; 13.
DR MINT; Q04233; -.
DR STRING; 4932.YML006C; -.
DR iPTMnet; Q04233; -.
DR MaxQB; Q04233; -.
DR PaxDb; Q04233; -.
DR PRIDE; Q04233; -.
DR EnsemblFungi; YML006C_mRNA; YML006C; YML006C.
DR GeneID; 855006; -.
DR KEGG; sce:YML006C; -.
DR SGD; S000004465; GIS4.
DR VEuPathDB; FungiDB:YML006C; -.
DR eggNOG; ENOG502QVAT; Eukaryota.
DR HOGENOM; CLU_016359_0_0_1; -.
DR InParanoid; Q04233; -.
DR OMA; NHENHYL; -.
DR BioCyc; YEAST:G3O-32611-MON; -.
DR PRO; PR:Q04233; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04233; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0006873; P:cellular ion homeostasis; IMP:SGD.
DR GO; GO:0035556; P:intracellular signal transduction; IGI:SGD.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..774
FT /note="Protein GIS4"
FT /id="PRO_0000087498"
FT REGION 145..269
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 281..322
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 338..367
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 387..423
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 557..603
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 637..701
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..222
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 233..248
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 296..322
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 557..571
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 576..591
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 637..696
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 165
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 304
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 616
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 622
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 707
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 720
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
SQ SEQUENCE 774 AA; 87140 MW; C7E837AA7001E241 CRC64;
MQKSVRVGDY FDNDDNGLWS WYLTNLRLGD FEELIGNQLK YTLLKRFLNS HFYGDNNISA
RPNKKILLVS IPENVHEDIS ILEIFLKDYF HLEKLEHIQI SKLTHSHCYN HENHYLLTDN
LNNFQDPTFL EFASTSWQVQ KNSKALNNNN RNSIPPPTIS SSKASNGKLE SNVSDDQWSN
INTQTSTATR TNTNTRTLTS PDTVDINVTS VNSQSNNNDT PQDNENEVDE EDATSSIVLN
FSHSRTVDSK PNRLPKIFPS YTNEDYTPSH SEIMSIDSFA GEDVSSTYPG QDLSLTTARR
EDESGQDEVE DHYSRVSHDL GDESIDQASY SMESSVSYTS YSSSSNSSSA HYSLSSSSRG
NPKRENIDHT NATYVSELSS ITSSIDNLTT STTPEEEDNL IHHNYDAQGY GSGEDDGEEV
YDDEDLSSSD YSVLSILPSI SICDSLGYFR LVLQSILIQD PDTKEIFTAI RQSNNKPTMA
SVTDDWLLYD SNFSMNNLQI LTLQDLLDIK RSFPKILFYT MVIVTNSGKQ VEEEFKNPNY
DNREGISKEQ PLDSELSLTN DPQQYFPTAY NNGYNDYIDD EDDEDDGDDA SLSEQSGPQM
YIPTRMESNV TTAHRSIRTV NSIGEWAFNR HNSVTKIDKS NSNELDNSKT GESTVLSSEP
HPMTQLSNSN TTSSNFSHSL KTKNSHKPNS KGNNESNSKN ELKKIKSSIN AMSAVERSKS
LPLPTLLKSL SGIDNPTHAT NKDRKRWKFQ MNRFKNHKNS GSAGTDKSQR CAIM
