ID   GIT1_SCHPO              Reviewed;        1098 AA.
AC   Q9P7K5;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=Adenylate cyclase activation protein git1;
DE   AltName: Full=Glucose-insensitive transcription protein 1;
GN   Name=git1; ORFNames=SPBC21C3.20c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   FUNCTION, INTERACTION WITH CYR1, AND SUBCELLULAR LOCATION.
RX   PubMed=16489217; DOI=10.1534/genetics.106.055699;
RA   Kao R.S., Morreale E., Wang L., Ivey F.D., Hoffman C.S.;
RT   "Schizosaccharomyces pombe Git1 is a C2-domain protein required for glucose
RT   activation of adenylate cyclase.";
RL   Genetics 173:49-61(2006).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- FUNCTION: Involved in the glucose/cAMP-signaling pathway. Required for
CC       maintaining basal cAMP levels and for producing a glucose-triggered
CC       cAMP response. {ECO:0000269|PubMed:16489217}.
CC   -!- SUBUNIT: Interacts with cyr1. {ECO:0000269|PubMed:16489217}.
CC   -!- INTERACTION:
CC       Q9P7K5; P14605: cyr1; NbExp=3; IntAct=EBI-1542810, EBI-1542835;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton,
CC       microtubule organizing center, spindle pole body. Note=Septum.
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DR   EMBL; CU329671; CAB76056.1; -; Genomic_DNA.
DR   PIR; T50364; T50364.
DR   RefSeq; NP_596600.1; NM_001022520.2.
DR   AlphaFoldDB; Q9P7K5; -.
DR   SMR; Q9P7K5; -.
DR   BioGRID; 277143; 9.
DR   IntAct; Q9P7K5; 1.
DR   STRING; 4896.SPBC21C3.20c.1; -.
DR   PaxDb; Q9P7K5; -.
DR   EnsemblFungi; SPBC21C3.20c.1; SPBC21C3.20c.1:pep; SPBC21C3.20c.
DR   GeneID; 2540617; -.
DR   KEGG; spo:SPBC21C3.20c; -.
DR   PomBase; SPBC21C3.20c; git1.
DR   VEuPathDB; FungiDB:SPBC21C3.20c; -.
DR   HOGENOM; CLU_283584_0_0_1; -.
DR   InParanoid; Q9P7K5; -.
DR   OMA; WRIHPGT; -.
DR   PhylomeDB; Q9P7K5; -.
DR   PRO; PR:Q9P7K5; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0032153; C:cell division site; HDA:PomBase.
DR   GO; GO:0005737; C:cytoplasm; IDA:PomBase.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0044732; C:mitotic spindle pole body; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0010619; P:adenylate cyclase-activating glucose-activated G protein-coupled receptor signaling pathway; IMP:PomBase.
DR   GO; GO:0010515; P:negative regulation of induction of conjugation with cellular fusion; IMP:PomBase.
DR   InterPro; IPR035892; C2_domain_sf.
DR   InterPro; IPR014770; Munc13_1.
DR   InterPro; IPR014772; Munc13_dom-2.
DR   SUPFAM; SSF49562; SSF49562; 1.
DR   PROSITE; PS51258; MHD1; 1.
DR   PROSITE; PS51259; MHD2; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Cytoskeleton; Nucleus; Reference proteome; Transducer.
FT   CHAIN           1..1098
FT                   /note="Adenylate cyclase activation protein git1"
FT                   /id="PRO_0000256253"
FT   DOMAIN          567..700
FT                   /note="MHD1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00587"
FT   DOMAIN          981..1097
FT                   /note="MHD2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00588"
SQ   SEQUENCE   1098 AA;  128300 MW;  8C3697EF3E16EFF2 CRC64;
     MGFTSVEPPD FFDWTARAIF LLSTLRSRRN AKQIERENAL LDQIKELLSI AIIQCHERNI
     PSIDLPPSFF NYVCEQLPRH PFAKIASSYI DTHTLFFQIS ISSFLCRFSN CSEDEIRLFK
     QHQSLDSFLL SLFDLLCSHH FSVEFTSISL IIKSSQWLFY TLSDYAHESL TGVEADITTK
     FLSTLADTWP EFEEQCTLSK HLFLLGDFSL ISYVKVLCGV FNIPNYNAFI LSVEDLLPEN
     ISFAAVADFQ LSKYRAINDN SVLRSDDFSS KAVFDKYVER ECYYSENAIS KIFQNYPDLA
     VLFEELQYAE GNNDLLSAIL NTNKYTLRPD DCAPYFYRLM DLCLRYCYSQ SNEIGTNVLW
     SMTTSFINIW RIHPGTCCCV VLSIYWPYLE DKLLKFSDLG DIFEYFFSLI HDCDYAIWSV
     HDEHQFSELL AFYHRRALND LVEELGSCPR SSDSCRAILD ILNNYIFSCP LFLVTPEELE
     EQYTDFFKKL KEKYGEFFQN LLKKELTGKP ESDLEGLRLV GVQLQATYEN LKSNFSARIF
     NQLSVHSFFA EATCHLFLEK LPTITKTYVY SNCKEIPDHI LEMINALYQQ TRALVACTGY
     LSSDYPKIYE AFSGTFVYRN LSLIIDKGLA NVLQTVLRES FIPKRSRFYS DSTKMILAPL
     QDCYDFLQKI DWPSSKDANR FYLHLGKLSE NYIHYYVDSM YALFIYSTDI PLKAEPLVPD
     LPVKQLKEKA SHFKRHSGDP NFLKLYRESF TLINTVSLVS GWLKMLHQRI DYKILTEKVM
     HFKDMEVPSA TVIISIKMAH LLVTPKAPYS FSVLLQDPES EFCVHTRDVY DELVPNWKET
     YQMEITNVKR FKLIIYQRNI HTGLETVYGH ATLEVDPDKL KQGHGKDVWL DITNKGQILI
     GLRVLLKRET PNYYCFAQME YLTVTEVKML NVLMISLSKF IRNSLRNSGL MDFVRYLDGD
     ENDNAEAETK EKMDELQARC EKEQQSLLAE LDDFFYWIYV NLLPKPLFYL LSRLWFGLLE
     TFRDILVPPV TDSTLRRIPL NKRELKMVYS WLQLFYDFFR NFQDIAPFDF LNTDEYKSVM
     AIKDYYFVKS KDVKRVSS