GKAP1_HUMAN
ID GKAP1_HUMAN Reviewed; 366 AA.
AC Q5VSY0; Q96LI0; Q9BYI1; Q9BYI2; Q9H225;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=G kinase-anchoring protein 1;
DE AltName: Full=cGMP-dependent protein kinase-anchoring protein of 42 kDa;
GN Name=GKAP1; Synonyms=GKAP42; ORFNames=FKSG21;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RX PubMed=10671526; DOI=10.1074/jbc.275.7.4897;
RA Yuasa K., Omori K., Yanaka N.;
RT "Binding and phosphorylation of a novel male germ cell-specific cGMP-
RT dependent protein kinase-anchoring protein by cGMP-dependent protein kinase
RT Ialpha.";
RL J. Biol. Chem. 275:4897-4905(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Wang Y.-G., Gong L.;
RT "Cloning of FKSG21, a novel gene located on human chromosome 9.";
RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 145-366 (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [7]
RP IDENTIFICATION.
RX PubMed=16015647; DOI=10.1002/gcc.20236;
RA Sweetser D.A., Peniket A.J., Haaland C., Blomberg A.A., Zhang Y.,
RA Zaidi S.T., Dayyani F., Zhao Z., Heerema N.A., Boultwood J., Dewald G.W.,
RA Paietta E., Slovak M.L., Willman C.L., Wainscoat J.S., Bernstein I.D.,
RA Daly S.B.;
RT "Delineation of the minimal commonly deleted segment and identification of
RT candidate tumor-suppressor genes in del(9q) acute myeloid leukemia.";
RL Genes Chromosomes Cancer 44:279-291(2005).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Regulates insulin-dependent IRS1 tyrosine phosphorylation in
CC adipocytes by modulating the availability of IRS1 to IR tyrosine
CC kinase. Its association with IRS1 is required for insulin-induced
CC translocation of SLC2A4 to the cell membrane. Involved in TNF-induced
CC impairment of insulin-dependent IRS1 tyrosine phosphorylation.
CC {ECO:0000250|UniProtKB:Q9JMB0}.
CC -!- SUBUNIT: Interacts with PRKG1 and IRS1. {ECO:0000250|UniProtKB:Q9JMB0}.
CC -!- INTERACTION:
CC Q5VSY0; B0YJ76: DYR; NbExp=3; IntAct=EBI-743722, EBI-10175413;
CC Q5VSY0; Q9H5Z6-2: FAM124B; NbExp=3; IntAct=EBI-743722, EBI-11986315;
CC Q5VSY0; Q5VSY0: GKAP1; NbExp=4; IntAct=EBI-743722, EBI-743722;
CC Q5VSY0; Q8N4P3: HDDC3; NbExp=3; IntAct=EBI-743722, EBI-750003;
CC Q5VSY0; Q63ZY3: KANK2; NbExp=5; IntAct=EBI-743722, EBI-2556193;
CC Q5VSY0; Q92993: KAT5; NbExp=3; IntAct=EBI-743722, EBI-399080;
CC Q5VSY0; Q969R5: L3MBTL2; NbExp=3; IntAct=EBI-743722, EBI-739909;
CC Q5VSY0; P55081: MFAP1; NbExp=3; IntAct=EBI-743722, EBI-1048159;
CC Q5VSY0; Q9BU76: MMTAG2; NbExp=3; IntAct=EBI-743722, EBI-742459;
CC Q5VSY0; Q8TCD6: PHOSPHO2; NbExp=3; IntAct=EBI-743722, EBI-2861380;
CC Q5VSY0; Q9P2K3-2: RCOR3; NbExp=3; IntAct=EBI-743722, EBI-1504830;
CC Q5VSY0; O00560: SDCBP; NbExp=3; IntAct=EBI-743722, EBI-727004;
CC Q5VSY0; Q8TBK6: ZCCHC10; NbExp=3; IntAct=EBI-743722, EBI-597063;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000250|UniProtKB:Q9JMB0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5VSY0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5VSY0-2; Sequence=VSP_030596;
CC -!- SIMILARITY: Belongs to the GKAP1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB71712.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=EAW62665.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB033131; BAB40454.1; -; mRNA.
DR EMBL; AB033132; BAB40455.1; -; mRNA.
DR EMBL; AF319476; AAG40320.1; -; mRNA.
DR EMBL; AL354733; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL662787; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471089; EAW62665.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CH471089; EAW62666.1; -; Genomic_DNA.
DR EMBL; BC014476; AAH14476.1; -; mRNA.
DR EMBL; AK058198; BAB71712.1; ALT_INIT; mRNA.
DR CCDS; CCDS35049.1; -. [Q5VSY0-1]
DR CCDS; CCDS47988.1; -. [Q5VSY0-2]
DR RefSeq; NP_001129425.1; NM_001135953.1. [Q5VSY0-2]
DR RefSeq; NP_079487.2; NM_025211.3. [Q5VSY0-1]
DR RefSeq; XP_005252298.1; XM_005252241.2. [Q5VSY0-1]
DR RefSeq; XP_011517360.1; XM_011519058.2. [Q5VSY0-1]
DR AlphaFoldDB; Q5VSY0; -.
DR SMR; Q5VSY0; -.
DR BioGRID; 123231; 49.
DR DIP; DIP-47324N; -.
DR IntAct; Q5VSY0; 29.
DR MINT; Q5VSY0; -.
DR STRING; 9606.ENSP00000365550; -.
DR iPTMnet; Q5VSY0; -.
DR PhosphoSitePlus; Q5VSY0; -.
DR BioMuta; GKAP1; -.
DR DMDM; 166224631; -.
DR EPD; Q5VSY0; -.
DR jPOST; Q5VSY0; -.
DR MassIVE; Q5VSY0; -.
DR MaxQB; Q5VSY0; -.
DR PaxDb; Q5VSY0; -.
DR PeptideAtlas; Q5VSY0; -.
DR PRIDE; Q5VSY0; -.
DR ProteomicsDB; 65286; -. [Q5VSY0-1]
DR ProteomicsDB; 65287; -. [Q5VSY0-2]
DR Antibodypedia; 27552; 171 antibodies from 23 providers.
DR DNASU; 80318; -.
DR Ensembl; ENST00000376365.7; ENSP00000365544.3; ENSG00000165113.13. [Q5VSY0-2]
DR Ensembl; ENST00000376371.7; ENSP00000365550.2; ENSG00000165113.13. [Q5VSY0-1]
DR GeneID; 80318; -.
DR KEGG; hsa:80318; -.
DR MANE-Select; ENST00000376371.7; ENSP00000365550.2; NM_025211.4; NP_079487.2.
DR UCSC; uc004amy.4; human. [Q5VSY0-1]
DR CTD; 80318; -.
DR DisGeNET; 80318; -.
DR GeneCards; GKAP1; -.
DR HGNC; HGNC:17496; GKAP1.
DR HPA; ENSG00000165113; Group enriched (skeletal muscle, testis).
DR MIM; 611356; gene.
DR neXtProt; NX_Q5VSY0; -.
DR OpenTargets; ENSG00000165113; -.
DR PharmGKB; PA134880503; -.
DR VEuPathDB; HostDB:ENSG00000165113; -.
DR eggNOG; ENOG502QUT6; Eukaryota.
DR GeneTree; ENSGT00390000008742; -.
DR HOGENOM; CLU_065161_1_0_1; -.
DR InParanoid; Q5VSY0; -.
DR OMA; RKNHQGR; -.
DR OrthoDB; 1595194at2759; -.
DR PhylomeDB; Q5VSY0; -.
DR TreeFam; TF328459; -.
DR PathwayCommons; Q5VSY0; -.
DR SignaLink; Q5VSY0; -.
DR SIGNOR; Q5VSY0; -.
DR BioGRID-ORCS; 80318; 21 hits in 1080 CRISPR screens.
DR ChiTaRS; GKAP1; human.
DR GenomeRNAi; 80318; -.
DR Pharos; Q5VSY0; Tdark.
DR PRO; PR:Q5VSY0; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q5VSY0; protein.
DR Bgee; ENSG00000165113; Expressed in sperm and 183 other tissues.
DR ExpressionAtlas; Q5VSY0; baseline and differential.
DR Genevisible; Q5VSY0; HS.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0046628; P:positive regulation of insulin receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; ISS:UniProtKB.
DR InterPro; IPR026109; GKAP1.
DR PANTHER; PTHR14899; PTHR14899; 1.
DR PRINTS; PR02083; GKINASEAP1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Golgi apparatus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..366
FT /note="G kinase-anchoring protein 1"
FT /id="PRO_0000315654"
FT REGION 1..95
FT /note="Interaction with IRS1"
FT /evidence="ECO:0000250|UniProtKB:Q9JMB0"
FT REGION 20..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 147..177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 47..77
FT /evidence="ECO:0000255"
FT COILED 128..160
FT /evidence="ECO:0000255"
FT COILED 243..353
FT /evidence="ECO:0000255"
FT COMPBIAS 86..105
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 23
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9JMB0"
FT MOD_RES 25
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9JMB0"
FT MOD_RES 27
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163"
FT MOD_RES 106
FT /note="Phosphoserine; by PKG"
FT /evidence="ECO:0000250|UniProtKB:Q9JMB0"
FT VAR_SEQ 196..246
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10671526"
FT /id="VSP_030596"
FT CONFLICT 325
FT /note="Q -> R (in Ref. 2; AAG40320)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 366 AA; 42078 MW; 973D16361756691B CRC64;
MASAVLSSVP TTASRFALLQ VDSGSGSDSE PGKGKGRNTG KSQTLGSKST TNEKKREKRR
KKKEQQQSEA NELRNLAFKK IPQKSSHAVC NAQHDLPLSN PVQKDSREEN WQEWRQRDEQ
LTSEMFEADL EKALLLSKLE YEEHKKEYED AENTSTQSKV MNKKDKRKNH QGKDRPLTVS
LKDFHSEDHI SKKTEELSSS QTLSHDGGFF NRLEDDVHKI LIREKRREQL TEYNGTDNCT
AHEHNQEVVL KDGRIERLKL ELERKDAEIQ KLKNVITQWE AKYKEVKARN AQLLKMLQEG
EMKDKAEILL QVDESQSIKN ELTIQVTSLH AALEQERSKV KVLQAELAKY QGGRKGKRNS
ESDQCR
