ID   GKAP1_RAT               Reviewed;         366 AA.
AC   Q5XIG5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=G kinase-anchoring protein 1;
GN   Name=Gkap1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23; SER-25 AND SER-27, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: Regulates insulin-dependent IRS1 tyrosine phosphorylation in
CC       adipocytes by modulating the availability of IRS1 to IR tyrosine
CC       kinase. Its association with IRS1 is required for insulin-induced
CC       translocation of SLC2A4 to the cell membrane. Involved in TNF-induced
CC       impairment of insulin-dependent IRS1 tyrosine phosphorylation.
CC       {ECO:0000250|UniProtKB:Q9JMB0}.
CC   -!- SUBUNIT: Interacts with PRKG1 and IRS1. {ECO:0000250|UniProtKB:Q9JMB0}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000250|UniProtKB:Q9JMB0}.
CC   -!- SIMILARITY: Belongs to the GKAP1 family. {ECO:0000305}.
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DR   EMBL; BC083717; AAH83717.1; -; mRNA.
DR   RefSeq; NP_001012160.1; NM_001012160.1.
DR   RefSeq; XP_006253621.1; XM_006253559.3.
DR   RefSeq; XP_017456077.1; XM_017600588.1.
DR   AlphaFoldDB; Q5XIG5; -.
DR   SMR; Q5XIG5; -.
DR   BioGRID; 262511; 1.
DR   IntAct; Q5XIG5; 2.
DR   STRING; 10116.ENSRNOP00000026070; -.
DR   iPTMnet; Q5XIG5; -.
DR   PhosphoSitePlus; Q5XIG5; -.
DR   PaxDb; Q5XIG5; -.
DR   PRIDE; Q5XIG5; -.
DR   GeneID; 361202; -.
DR   KEGG; rno:361202; -.
DR   UCSC; RGD:1306955; rat.
DR   CTD; 80318; -.
DR   RGD; 1306955; Gkap1.
DR   VEuPathDB; HostDB:ENSRNOG00000019272; -.
DR   eggNOG; ENOG502QUT6; Eukaryota.
DR   HOGENOM; CLU_065161_1_0_1; -.
DR   InParanoid; Q5XIG5; -.
DR   OMA; RKNHQGR; -.
DR   OrthoDB; 1595194at2759; -.
DR   PhylomeDB; Q5XIG5; -.
DR   TreeFam; TF328459; -.
DR   PRO; PR:Q5XIG5; -.
DR   Proteomes; UP000002494; Chromosome 17.
DR   Bgee; ENSRNOG00000019272; Expressed in testis and 20 other tissues.
DR   Genevisible; Q5XIG5; RN.
DR   GO; GO:0005794; C:Golgi apparatus; ISO:RGD.
DR   GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR   GO; GO:0046628; P:positive regulation of insulin receptor signaling pathway; ISS:UniProtKB.
DR   GO; GO:0007165; P:signal transduction; ISO:RGD.
DR   InterPro; IPR026109; GKAP1.
DR   PANTHER; PTHR14899; PTHR14899; 1.
DR   PRINTS; PR02083; GKINASEAP1.
PE   1: Evidence at protein level;
KW   Coiled coil; Golgi apparatus; Phosphoprotein; Reference proteome.
FT   CHAIN           1..366
FT                   /note="G kinase-anchoring protein 1"
FT                   /id="PRO_0000315656"
FT   REGION          1..95
FT                   /note="Interaction with IRS1"
FT                   /evidence="ECO:0000250|UniProtKB:Q9JMB0"
FT   REGION          20..105
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          46..77
FT                   /evidence="ECO:0000255"
FT   COILED          129..160
FT                   /evidence="ECO:0000255"
FT   COILED          250..299
FT                   /evidence="ECO:0000255"
FT   COILED          326..353
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        81..105
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         23
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         25
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         27
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         106
FT                   /note="Phosphoserine; by PKG"
FT                   /evidence="ECO:0000250|UniProtKB:Q9JMB0"
SQ   SEQUENCE   366 AA;  41932 MW;  435F1B1618361129 CRC64;
     MASAVLSSVL TTASRFALLQ VDSGSGSDSE PGKGKGRNNG KSQTLGNKST ANEKKREKRR
     KKKEQQQSEA NELRNLAFKK IPQKSSHSVC NVQHELSLPN PVQKESREEN WQEWRQRDEQ
     LTSEMFEADL EKALLLSKLE YEEHRQDYEN AENASTQTKV INKKDKRKNH QGKDKPLTVS
     LKDFQCEDHI SKKTEESNSS QPLSHDGGFF NRLEDDVHKI LVREKRREQL TEHNGTDNCP
     APEHNQEVGL KDGRIERLKL ELERKDAEIQ KLKAVVTQWE AKYKEVKARN GQLLKMLQEG
     EMKDKAEILL QVDESQSIKN ELTVQVSSLH AALEQERSKV KVLQAELAKY QGGRKGKRNC
     EPDQCR