GKN1_HUMAN
ID GKN1_HUMAN Reviewed; 185 AA.
AC Q9NS71; Q8IUA9;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 29-SEP-2021, sequence version 4.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Gastrokine-1;
DE AltName: Full=18 kDa antrum mucosa protein;
DE Short=AMP-18;
DE AltName: Full=Protein CA11;
DE Flags: Precursor;
GN Name=GKN1; Synonyms=AMP18, CA11; ORFNames=UNQ489/PRO1005;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Stomach;
RX PubMed=10835488; DOI=10.1111/j.1349-7006.2000.tb00967.x;
RA Yoshikawa Y., Mukai H., Hino F., Asada K., Kato I.;
RT "Isolation of two novel genes, down-regulated in gastric cancer.";
RL Jpn. J. Cancer Res. 91:459-463(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Stomach;
RX PubMed=12851218; DOI=10.1152/ajpgi.00453.2002;
RA Martin T.E., Powell C.T., Wang Z., Bhattacharyya S., Walsh-Reitz M.M.,
RA Agarwal K., Toback F.G.;
RT "A novel mitogenic protein that is highly expressed in cells of the gastric
RT antrum mucosa.";
RL Am. J. Physiol. 285:G332-G343(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [6]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=15221938; DOI=10.1002/path.1583;
RA Oien K.A., McGregor F., Butler S., Ferrier R.K., Downie I., Bryce S.,
RA Burns S., Keith W.N.;
RT "Gastrokine 1 is abundantly and specifically expressed in superficial
RT gastric epithelium, down-regulated in gastric carcinoma, and shows high
RT evolutionary conservation.";
RL J. Pathol. 203:789-797(2004).
RN [7]
RP VARIANT [LARGE SCALE ANALYSIS] THR-104.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: Has mitogenic activity and may be involved in maintaining the
CC integrity of the gastric mucosal epithelium.
CC {ECO:0000269|PubMed:12851218}.
CC -!- INTERACTION:
CC Q9NS71; Q13520: AQP6; NbExp=3; IntAct=EBI-3933251, EBI-13059134;
CC Q9NS71; Q5JX71: FAM209A; NbExp=3; IntAct=EBI-3933251, EBI-18304435;
CC Q9NS71; Q53GS7: GLE1; NbExp=3; IntAct=EBI-3933251, EBI-1955541;
CC Q9NS71; O14901: KLF11; NbExp=3; IntAct=EBI-3933251, EBI-948266;
CC Q9NS71; Q9GZY8-5: MFF; NbExp=3; IntAct=EBI-3933251, EBI-11956541;
CC Q9NS71; Q9BVL2: NUP58; NbExp=3; IntAct=EBI-3933251, EBI-2811583;
CC Q9NS71; Q8IY26: PLPP6; NbExp=3; IntAct=EBI-3933251, EBI-11721828;
CC Q9NS71; Q9Y6X1: SERP1; NbExp=3; IntAct=EBI-3933251, EBI-10329948;
CC Q9NS71; Q8N6R1: SERP2; NbExp=3; IntAct=EBI-3933251, EBI-749270;
CC Q9NS71; Q9NRQ5: SMCO4; NbExp=3; IntAct=EBI-3933251, EBI-8640191;
CC Q9NS71; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-3933251, EBI-5235340;
CC Q9NS71; Q7Z7N9: TMEM179B; NbExp=3; IntAct=EBI-3933251, EBI-11724423;
CC Q9NS71; Q9NSU2-1: TREX1; NbExp=3; IntAct=EBI-3933251, EBI-16746122;
CC Q9NS71; Q9Y385: UBE2J1; NbExp=3; IntAct=EBI-3933251, EBI-988826;
CC Q9NS71; Q9P0L0: VAPA; NbExp=3; IntAct=EBI-3933251, EBI-1059156;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15221938}.
CC Cytoplasmic granule {ECO:0000269|PubMed:15221938}. Golgi apparatus
CC {ECO:0000305|PubMed:15221938}. Note=Shows abundant granular cytoplasmic
CC staining, with perinuclear accentuation suggestive of the Golgi
CC apparatus. {ECO:0000269|PubMed:15221938}.
CC -!- TISSUE SPECIFICITY: Expressed in stomach (at protein level). No
CC expression is detected in cancer tissue or gastric cancer cell lines.
CC {ECO:0000269|PubMed:10835488, ECO:0000269|PubMed:12851218,
CC ECO:0000269|PubMed:15221938}.
CC -!- SIMILARITY: Belongs to the gastrokine family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH59778.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAA92433.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB039886; BAA92433.1; ALT_INIT; mRNA.
DR EMBL; AY139182; AAN75447.1; -; Genomic_DNA.
DR EMBL; AY139184; AAN75449.1; -; mRNA.
DR EMBL; AC114802; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC059778; AAH59778.2; ALT_INIT; mRNA.
DR EMBL; AY359050; AAQ89409.1; -; mRNA.
DR CCDS; CCDS1891.2; -.
DR RefSeq; NP_062563.3; NM_019617.3.
DR AlphaFoldDB; Q9NS71; -.
DR BioGRID; 121133; 29.
DR IntAct; Q9NS71; 25.
DR STRING; 9606.ENSP00000367172; -.
DR TCDB; 1.C.81.1.10; the arenicin (arenicin) family.
DR iPTMnet; Q9NS71; -.
DR PhosphoSitePlus; Q9NS71; -.
DR BioMuta; GKN1; -.
DR DMDM; 223590223; -.
DR MassIVE; Q9NS71; -.
DR PaxDb; Q9NS71; -.
DR PeptideAtlas; Q9NS71; -.
DR PRIDE; Q9NS71; -.
DR ProteomicsDB; 82501; -.
DR Antibodypedia; 30965; 329 antibodies from 27 providers.
DR DNASU; 56287; -.
DR Ensembl; ENST00000377938.4; ENSP00000367172.3; ENSG00000169605.7.
DR GeneID; 56287; -.
DR KEGG; hsa:56287; -.
DR MANE-Select; ENST00000377938.4; ENSP00000367172.3; NM_019617.4; NP_062563.4.
DR UCSC; uc002sfc.4; human.
DR CTD; 56287; -.
DR DisGeNET; 56287; -.
DR GeneCards; GKN1; -.
DR HGNC; HGNC:23217; GKN1.
DR HPA; ENSG00000169605; Tissue enriched (stomach).
DR MIM; 606402; gene.
DR neXtProt; NX_Q9NS71; -.
DR OpenTargets; ENSG00000169605; -.
DR PharmGKB; PA134898851; -.
DR VEuPathDB; HostDB:ENSG00000169605; -.
DR eggNOG; ENOG502S4AB; Eukaryota.
DR GeneTree; ENSGT00930000150969; -.
DR HOGENOM; CLU_098684_2_0_1; -.
DR InParanoid; Q9NS71; -.
DR OrthoDB; 1464387at2759; -.
DR PhylomeDB; Q9NS71; -.
DR TreeFam; TF335950; -.
DR PathwayCommons; Q9NS71; -.
DR SignaLink; Q9NS71; -.
DR SIGNOR; Q9NS71; -.
DR BioGRID-ORCS; 56287; 12 hits in 1070 CRISPR screens.
DR ChiTaRS; GKN1; human.
DR GenomeRNAi; 56287; -.
DR Pharos; Q9NS71; Tbio.
DR PRO; PR:Q9NS71; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q9NS71; protein.
DR Bgee; ENSG00000169605; Expressed in mucosa of stomach and 92 other tissues.
DR ExpressionAtlas; Q9NS71; baseline and differential.
DR Genevisible; Q9NS71; HS.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; TAS:UniProtKB.
DR GO; GO:0007586; P:digestion; NAS:UniProtKB.
DR GO; GO:0051781; P:positive regulation of cell division; IEA:UniProtKB-KW.
DR GO; GO:0042127; P:regulation of cell population proliferation; IBA:GO_Central.
DR InterPro; IPR007084; BRICHOS_dom.
DR Pfam; PF04089; BRICHOS; 1.
DR SMART; SM01039; BRICHOS; 1.
DR PROSITE; PS50869; BRICHOS; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Golgi apparatus; Mitogen; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..185
FT /note="Gastrokine-1"
FT /id="PRO_0000021332"
FT DOMAIN 54..150
FT /note="BRICHOS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00255"
FT DISULFID 81..142
FT /evidence="ECO:0000250"
FT VARIANT 104
FT /note="K -> T (in a breast cancer sample; somatic
FT mutation)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_035923"
SQ SEQUENCE 185 AA; 20331 MW; 0387C4CAB2A2DC02 CRC64;
MKFTIVFAGL LGVFLAPALA NYNINVNDDN NNAGSGQQSV SVNNEHNVAN VDNNNGWDSW
NSIWDYGNGF AATRLFQKKT CIVHKMNKEV MPSIQSLDAL VKEKKLQGKG PGGPPPKGLM
YSVNPNKVDD LSKFGKNIAN MCRGIPTYMA EEMQEASLFF YSGTCYTTSV LWIVDISFCG
DTVEN
