GKN2_MOUSE
ID GKN2_MOUSE Reviewed; 184 AA.
AC Q9CQS6;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Gastrokine-2;
DE AltName: Full=Blottin;
DE Flags: Precursor;
GN Name=Gkn2; Synonyms=Blot;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, INTERACTION WITH
RP TFF2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=C57BL/6J, and FVB/NJ; TISSUE=Foveolar epithelium;
RX PubMed=16888721; DOI=10.1002/pmic.200500911;
RA Otto W.R., Patel K., McKinnell I., Evans M.D., Lee C.-Y., Frith D.,
RA Hanrahan S., Blight K., Blin N., Kayademir T., Poulsom R., Jeffery R.,
RA Hunt T., Wright N.A., McGregor F., Oien K.A.;
RT "Identification of blottin: a novel gastric trefoil factor family-2 binding
RT protein.";
RL Proteomics 6:4235-4245(2006).
RN [2] {ECO:0000312|EMBL:BAB25046.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAB25046.1};
RC TISSUE=Pancreas {ECO:0000312|EMBL:BAB25046.1}, and
RC Stomach {ECO:0000312|EMBL:BAB26008.1};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3] {ECO:0000312|EMBL:AAH99445.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:AAH99445.1};
RC TISSUE=Placenta {ECO:0000312|EMBL:AAH99445.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBUNIT: Heterodimer with TFF1; disulfide linked (By similarity).
CC Interacts with TFF2. {ECO:0000250, ECO:0000269|PubMed:16888721}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16888721}. Golgi
CC apparatus {ECO:0000269|PubMed:16888721}. Note=Secreted into the mucus
CC layer, also present in Golgi apparatus and mucus granules.
CC -!- TISSUE SPECIFICITY: Stomach foveolar epithelium and duodenal Brunner's
CC glands. {ECO:0000269|PubMed:16888721}.
CC -!- DEVELOPMENTAL STAGE: Expressed from 17.5 dpc onwards.
CC {ECO:0000269|PubMed:16888721}.
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DR EMBL; AY943909; AAY24522.1; -; mRNA.
DR EMBL; AK007451; BAB25046.1; -; mRNA.
DR EMBL; AK007705; BAB25201.1; -; mRNA.
DR EMBL; AK008986; BAB26008.1; -; mRNA.
DR EMBL; BC099445; AAH99445.1; -; mRNA.
DR CCDS; CCDS39546.1; -.
DR RefSeq; NP_079743.1; NM_025467.1.
DR AlphaFoldDB; Q9CQS6; -.
DR SMR; Q9CQS6; -.
DR STRING; 10090.ENSMUSP00000032128; -.
DR PhosphoSitePlus; Q9CQS6; -.
DR MaxQB; Q9CQS6; -.
DR PaxDb; Q9CQS6; -.
DR PRIDE; Q9CQS6; -.
DR ProteomicsDB; 265751; -.
DR Antibodypedia; 56589; 111 antibodies from 20 providers.
DR DNASU; 66284; -.
DR Ensembl; ENSMUST00000032128; ENSMUSP00000032128; ENSMUSG00000030049.
DR GeneID; 66284; -.
DR KEGG; mmu:66284; -.
DR UCSC; uc009cti.1; mouse.
DR CTD; 200504; -.
DR MGI; MGI:1913534; Gkn2.
DR VEuPathDB; HostDB:ENSMUSG00000030049; -.
DR eggNOG; ENOG502SNS6; Eukaryota.
DR GeneTree; ENSGT00930000150969; -.
DR HOGENOM; CLU_098684_2_0_1; -.
DR InParanoid; Q9CQS6; -.
DR OMA; IFGTQSH; -.
DR OrthoDB; 1298343at2759; -.
DR PhylomeDB; Q9CQS6; -.
DR TreeFam; TF335950; -.
DR BioGRID-ORCS; 66284; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Gkn2; mouse.
DR PRO; PR:Q9CQS6; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9CQS6; protein.
DR Bgee; ENSMUSG00000030049; Expressed in epithelium of stomach and 33 other tissues.
DR Genevisible; Q9CQS6; MM.
DR GO; GO:0045178; C:basal part of cell; IDA:MGI.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0042127; P:regulation of cell population proliferation; IBA:GO_Central.
DR GO; GO:0009617; P:response to bacterium; IEP:MGI.
DR InterPro; IPR007084; BRICHOS_dom.
DR Pfam; PF04089; BRICHOS; 1.
DR SMART; SM01039; BRICHOS; 1.
DR PROSITE; PS50869; BRICHOS; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Golgi apparatus;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..184
FT /note="Gastrokine-2"
FT /id="PRO_0000252105"
FT DOMAIN 54..151
FT /note="BRICHOS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00255"
FT DISULFID 81..143
FT /evidence="ECO:0000250"
SQ SEQUENCE 184 AA; 20469 MW; 612A18FABE652230 CRC64;
MKPLVAFLVV LSIFGIQSQA EEIFNIFVPS KNGGNIQETV TIDNQQNTAT INIHSGSCSS
TTIFDYKHGY IASRVLSRRA CYVIKMDHKA IPALDKLQRF LYEKQTMNAI DSPEYTWVRY
NPLKSLITKV DWFLFGSPIR QLCKHMPLYE GEVATKPKEV STGACAKVGL LGILGVSICG
GIHL
