GKN3_MOUSE
ID GKN3_MOUSE Reviewed; 191 AA.
AC Q9D0T7;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Gastrokine-3;
DE Flags: Precursor;
GN Name=Gkn3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP MUTAGENESIS OF CYS-147 AND CYS-171, AND FUNCTION.
RC STRAIN=C57BL/6J; TISSUE=Stomach;
RX PubMed=20138039; DOI=10.1053/j.gastro.2010.01.050;
RA Menheniott T.R., Peterson A.J., O'Connor L., Lee K.S., Kalantzis A.,
RA Kondova I., Bontrop R.E., Bell K.M., Giraud A.S.;
RT "A novel gastrokine, Gkn3, marks gastric atrophy and shows evidence of
RT adaptive gene loss in humans.";
RL Gastroenterology 138:1823-1835(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Inhibits gastric epithelial cell proliferation.
CC {ECO:0000269|PubMed:20138039}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20138039}.
CC -!- TISSUE SPECIFICITY: Expressed in stomach. Present in mucus cells at the
CC base of antral glands, and Brunner glands of the duodenum. Present at
CC lower levels in the mucus neck cell region of the fundus (at protein
CC level). {ECO:0000269|PubMed:20138039}.
CC -!- SIMILARITY: Belongs to the gastrokine family. {ECO:0000305}.
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DR EMBL; GU220566; ADB22387.1; -; mRNA.
DR EMBL; FN600638; CBI70313.1; -; mRNA.
DR EMBL; AK004474; BAB23320.1; -; mRNA.
DR EMBL; CH466523; EDK99205.1; -; Genomic_DNA.
DR EMBL; BC116955; AAI16956.1; -; mRNA.
DR CCDS; CCDS20320.1; -.
DR RefSeq; NP_081136.1; NM_026860.1.
DR AlphaFoldDB; Q9D0T7; -.
DR STRING; 10090.ENSMUSP00000032127; -.
DR GlyGen; Q9D0T7; 1 site.
DR iPTMnet; Q9D0T7; -.
DR PhosphoSitePlus; Q9D0T7; -.
DR MaxQB; Q9D0T7; -.
DR PaxDb; Q9D0T7; -.
DR PRIDE; Q9D0T7; -.
DR ProteomicsDB; 271224; -.
DR Ensembl; ENSMUST00000032127; ENSMUSP00000032127; ENSMUSG00000030048.
DR GeneID; 68888; -.
DR KEGG; mmu:68888; -.
DR UCSC; uc009ctj.1; mouse.
DR CTD; 68888; -.
DR MGI; MGI:1916138; Gkn3.
DR VEuPathDB; HostDB:ENSMUSG00000030048; -.
DR eggNOG; ENOG502S4AB; Eukaryota.
DR GeneTree; ENSGT00930000150969; -.
DR HOGENOM; CLU_098684_1_0_1; -.
DR InParanoid; Q9D0T7; -.
DR OMA; FSKMACV; -.
DR OrthoDB; 1205514at2759; -.
DR PhylomeDB; Q9D0T7; -.
DR TreeFam; TF335950; -.
DR BioGRID-ORCS; 68888; 5 hits in 73 CRISPR screens.
DR ChiTaRS; Gkn3; mouse.
DR PRO; PR:Q9D0T7; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9D0T7; protein.
DR Bgee; ENSMUSG00000030048; Expressed in epithelium of stomach and 123 other tissues.
DR Genevisible; Q9D0T7; MM.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0050680; P:negative regulation of epithelial cell proliferation; IDA:MGI.
DR GO; GO:0042127; P:regulation of cell population proliferation; IBA:GO_Central.
DR InterPro; IPR007084; BRICHOS_dom.
DR Pfam; PF04089; BRICHOS; 1.
DR SMART; SM01039; BRICHOS; 1.
DR PROSITE; PS50869; BRICHOS; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..191
FT /note="Gastrokine-3"
FT /id="PRO_0000394474"
FT DOMAIN 63..155
FT /note="BRICHOS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00255"
FT CARBOHYD 33
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 90..147
FT /evidence="ECO:0000250"
FT MUTAGEN 147
FT /note="C->G: Impaired secretion."
FT /evidence="ECO:0000269|PubMed:20138039"
FT MUTAGEN 171
FT /note="C->G: Impaired secretion."
FT /evidence="ECO:0000269|PubMed:20138039"
SQ SEQUENCE 191 AA; 20772 MW; 76D7DB4796AE8B4D CRC64;
MPLHSLERDN MRRLIAPSIL VTVFLVPALA LTNTSDSYPL DGSVGTQTIH VDALRGVVSI
RDNSVQSEWD GVMDYKNDLL AAKLFSKMAC VLAKMDPAAF PSLDDITQAL GKQASGHYPP
TRGLTYTVLP SRIKNLAQYG VPIKDLCRAV PTYFARQQKE GTALTMDPDS CSELQLLSFM
GLSICGEIPG L
