GL1_ARATH
ID GL1_ARATH Reviewed; 228 AA.
AC P27900; Q39109; Q53YQ0; Q93V34; Q93VW0; Q93WP0; Q947R6; Q9LF81; Q9LF82;
AC Q9ST42;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 18-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Trichome differentiation protein GL1 {ECO:0000303|PubMed:1934056};
DE AltName: Full=Myb-related protein 0;
DE Short=AtMYB0;
DE AltName: Full=Protein GLABRA 1 {ECO:0000303|PubMed:11063707};
DE AltName: Full=Protein GLABROUS 1 {ECO:0000303|PubMed:10520748};
DE AltName: Full=R2R3-MYB transcription factor GL1 {ECO:0000303|PubMed:11597504};
GN Name=GL1 {ECO:0000303|PubMed:1934056}; Synonyms=MYB0;
GN OrderedLocusNames=At3g27920 {ECO:0000312|Araport:AT3G27920};
GN ORFNames=K16N12.17 {ECO:0000312|EMBL:BAB02538.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1934056; DOI=10.1016/0092-8674(91)90523-2;
RA Oppenheimer D.G., Herman P.L., Sivakumaran S., Esch J.J., Marks M.D.;
RT "A myb gene required for leaf trichome differentiation in Arabidopsis is
RT expressed in stipules.";
RL Cell 67:483-493(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTAGENESIS OF 182-GLN--LEU-228 AND
RP 202-LEU--LEU-228.
RX PubMed=8111017; DOI=10.1007/bf00040586;
RA Esch J.J., Oppenheimer D.G., Marks M.D.;
RT "Characterization of a weak allele of the GL1 gene of Arabidopsis
RT thaliana.";
RL Plant Mol. Biol. 24:203-207(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10520748; DOI=10.3109/10425179809072193;
RA Shikazono N., Tanaka A., Yokota Y., Watanabe H., Tano S.;
RT "Nucleotide sequence of the GLABROUS1 gene of Arabidopsis thaliana ecotype
RT Columbia.";
RL DNA Seq. 9:177-181(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-100, AND VARIANTS
RP 130-VAL--GLU-132; SER-148 DEL; ARG-149; ARG-176 AND 222-SER--ILE-224.
RC STRAIN=cv. Aa-0, cv. An-1, cv. Ba-1, cv. Bla-1, cv. Bur-0, cv. Can-0,
RC cv. Columbia, cv. Di-0, cv. Es-0, cv. Gr-1, cv. Hi-0, cv. Ita-0, cv. Kas-1,
RC cv. Kon, cv. Landsberg erecta, cv. Ms-0, cv. Mt-0, cv. Nd-0, cv. No-0,
RC cv. Oy-0, cv. RLD, cv. Sha, cv. Te-0, cv. Tsu-0, cv. Wassilewskija, and
RC cv. Yo-0;
RX PubMed=11504855; DOI=10.1093/oxfordjournals.molbev.a003963;
RA Hauser M.-T., Harr B., Schloetterer C.;
RT "Trichome distribution in Arabidopsis thaliana and its close relative
RT Arabidopsis lyrata: molecular analysis of the candidate gene GLABROUS1.";
RL Mol. Biol. Evol. 18:1754-1763(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L.-J., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [8]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [9]
RP FUNCTION, INDUCTION BY GIBBERELLINS, AND TISSUE SPECIFICITY.
RX PubMed=9625690; DOI=10.1104/pp.117.2.375;
RA Perazza D., Vachon G., Herzog M.;
RT "Gibberellins promote trichome formation by Up-regulating GLABROUS1 in
RT arabidopsis.";
RL Plant Physiol. 117:375-383(1998).
RN [10]
RP FUNCTION, AND INTERACTION WITH GL3.
RX PubMed=11063707; DOI=10.1093/genetics/156.3.1349;
RA Payne C.T., Zhang F., Lloyd A.M.;
RT "GL3 encodes a bHLH protein that regulates trichome development in
RT arabidopsis through interaction with GL1 and TTG1.";
RL Genetics 156:1349-1362(2000).
RN [11]
RP TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta; TISSUE=Silique;
RX PubMed=11437443; DOI=10.1006/dbio.2001.0287;
RA Kirik V., Schnittger A., Radchuk V., Adler K., Huelskamp M., Baeumlein H.;
RT "Ectopic expression of the Arabidopsis AtMYB23 gene induces differentiation
RT of trichome cells.";
RL Dev. Biol. 235:366-377(2001).
RN [12]
RP FUNCTION.
RC STRAIN=cv. Columbia; TISSUE=Root;
RX PubMed=12356720; DOI=10.1093/emboj/cdf524;
RA Schellmann S., Schnittger A., Kirik V., Wada T., Okada K., Beermann A.,
RA Thumfahrt J., Juergens G., Huelskamp M.;
RT "TRIPTYCHON and CAPRICE mediate lateral inhibition during trichome and root
RT hair patterning in Arabidopsis.";
RL EMBO J. 21:5036-5046(2002).
RN [13]
RP INTERACTION WITH BHLH2.
RX PubMed=12917293; DOI=10.1242/dev.00681;
RA Zhang F., Gonzalez A., Zhao M., Payne C.T., Lloyd A.M.;
RT "A network of redundant bHLH proteins functions in all TTG1-dependent
RT pathways of Arabidopsis.";
RL Development 130:4859-4869(2003).
RN [14]
RP INTERACTION WITH GL3, AND SUBCELLULAR LOCATION.
RX PubMed=14561633; DOI=10.1242/dev.00812;
RA Esch J.J., Chen M., Sanders M., Hillestad M., Ndkium S., Idelkope B.,
RA Neizer J., Marks M.D.;
RT "A contradictory GLABRA3 allele helps define gene interactions controlling
RT trichome development in Arabidopsis.";
RL Development 130:5885-5894(2003).
RN [15]
RP INDUCTION BY GEBP.
RX PubMed=12535344; DOI=10.1046/j.1365-313x.2003.01622.x;
RA Curaba J., Herzog M., Vachon G.;
RT "GeBP, the first member of a new gene family in Arabidopsis, encodes a
RT nuclear protein with DNA-binding activity and is regulated by KNAT1.";
RL Plant J. 33:305-317(2003).
RN [16]
RP FUNCTION, AND INTERACTION WITH BHLH2 AND BHLH12.
RX PubMed=15361138; DOI=10.1111/j.1365-313x.2004.02183.x;
RA Zimmermann I.M., Heim M.A., Weisshaar B., Uhrig J.F.;
RT "Comprehensive identification of Arabidopsis thaliana MYB transcription
RT factors interacting with R/B-like BHLH proteins.";
RL Plant J. 40:22-34(2004).
RN [17]
RP FUNCTION, INTERACTION WITH GL3, AND DEVELOPMENTAL STAGE.
RX PubMed=15728674; DOI=10.1242/dev.01708;
RA Kirik V., Lee M.M., Wester K., Herrmann U., Zheng Z., Oppenheimer D.,
RA Schiefelbein J., Hulskamp M.;
RT "Functional diversification of MYB23 and GL1 genes in trichome
RT morphogenesis and initiation.";
RL Development 132:1477-1485(2005).
RN [18]
RP GENE FAMILY.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
RN [19]
RP INTERACTION WITH MYB82, AND SUBUNIT.
RC STRAIN=cv. Columbia;
RX PubMed=24803498; DOI=10.1093/jxb/eru179;
RA Liang G., He H., Li Y., Ai Q., Yu D.;
RT "MYB82 functions in regulation of trichome development in Arabidopsis.";
RL J. Exp. Bot. 65:3215-3223(2014).
CC -!- FUNCTION: Transcription activator, when associated with
CC BHLH2/EGL3/MYC146 or BHLH12/MYC1. Involved in epidermal cell fate
CC specification in leaves. Together with TTG1 and GL3, promotes trichome
CC formation and endoreplication. Regulates the production of a signal
CC that induces hair (trichome) precursor cells on leaf primordia to
CC differentiate. Binds to the WER-binding sites (WBS) promoter regions
CC and activates the transcription of target genes (By similarity).
CC {ECO:0000250, ECO:0000269|PubMed:11063707, ECO:0000269|PubMed:12356720,
CC ECO:0000269|PubMed:15361138, ECO:0000269|PubMed:15728674,
CC ECO:0000269|PubMed:9625690}.
CC -!- SUBUNIT: Homodimer and heterodimer with MYB82 (PubMed:24803498).
CC Interacts directly with GL3 and BHLH2. Part of a complex made of GL1,
CC GL3 or BHLH2, and TTG1. Interacts also with BHLH2/EGL3/MYC146 and
CC BHLH12/MYC1. Interacts with MYB82 (PubMed:24803498).
CC {ECO:0000269|PubMed:11063707, ECO:0000269|PubMed:12917293,
CC ECO:0000269|PubMed:14561633, ECO:0000269|PubMed:15361138,
CC ECO:0000269|PubMed:15728674, ECO:0000269|PubMed:24803498}.
CC -!- INTERACTION:
CC P27900; Q9FN69: GL3; NbExp=5; IntAct=EBI-1543742, EBI-533348;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:14561633}. Note=Detected in trichome nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, stems and flowers
CC (PubMed:11437443). Expressed in trichome cells and in leaf primordia
CC (PubMed:9625690). {ECO:0000269|PubMed:11437443,
CC ECO:0000269|PubMed:9625690}.
CC -!- DEVELOPMENTAL STAGE: Ubiquitous in young leaves primordia. Becomes more
CC prominent in developing trichome cells but disappears progressively
CC when trichomes begin to initiate branches.
CC {ECO:0000269|PubMed:15728674}.
CC -!- INDUCTION: Up-regulated by gibberellins (PubMed:9625690). May be
CC regulated by GEBP and GEBP-like proteins (PubMed:12535344).
CC {ECO:0000269|PubMed:12535344, ECO:0000269|PubMed:9625690}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=More to it than meets the
CC finger - Issue 120 of August 2010;
CC URL="https://web.expasy.org/spotlight/back_issues/120";
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DR EMBL; M79448; AAC97387.1; -; Genomic_DNA.
DR EMBL; L22786; AAC97388.1; -; Genomic_DNA.
DR EMBL; AB006078; BAA86879.1; -; Genomic_DNA.
DR EMBL; AJ243899; CAB97484.1; -; Genomic_DNA.
DR EMBL; AJ243900; CAB97485.1; -; Genomic_DNA.
DR EMBL; AF263690; AAL01215.1; -; Genomic_DNA.
DR EMBL; AF263691; AAL01216.1; -; Genomic_DNA.
DR EMBL; AF263692; AAL01217.1; -; Genomic_DNA.
DR EMBL; AF263693; AAL01218.1; -; Genomic_DNA.
DR EMBL; AF263694; AAL01219.1; -; Genomic_DNA.
DR EMBL; AF263695; AAL01220.1; -; Genomic_DNA.
DR EMBL; AF263696; AAL01221.1; -; Genomic_DNA.
DR EMBL; AF263697; AAL01222.1; -; Genomic_DNA.
DR EMBL; AF263698; AAL01223.1; -; Genomic_DNA.
DR EMBL; AF263699; AAL01224.1; -; Genomic_DNA.
DR EMBL; AF263700; AAL01225.1; -; Genomic_DNA.
DR EMBL; AF263701; AAL01226.1; -; Genomic_DNA.
DR EMBL; AF263702; AAL01227.1; -; Genomic_DNA.
DR EMBL; AF263703; AAL01228.1; -; Genomic_DNA.
DR EMBL; AF263704; AAL01229.1; -; Genomic_DNA.
DR EMBL; AF263705; AAL01230.1; -; Genomic_DNA.
DR EMBL; AF263706; AAL01231.1; -; Genomic_DNA.
DR EMBL; AF263707; AAL01232.1; -; Genomic_DNA.
DR EMBL; AF263708; AAL01233.1; -; Genomic_DNA.
DR EMBL; AF263709; AAL01234.1; -; Genomic_DNA.
DR EMBL; AF263710; AAL01235.1; -; Genomic_DNA.
DR EMBL; AF263711; AAL01236.1; -; Genomic_DNA.
DR EMBL; AF263712; AAL01237.1; -; Genomic_DNA.
DR EMBL; AF263713; AAL01238.1; -; Genomic_DNA.
DR EMBL; AF263714; AAL01239.1; -; Genomic_DNA.
DR EMBL; AF263715; AAL01240.1; -; Genomic_DNA.
DR EMBL; AF263718; AAL01243.1; -; Genomic_DNA.
DR EMBL; AF263719; AAL01244.1; -; Genomic_DNA.
DR EMBL; AF495524; AAM14620.1; -; mRNA.
DR EMBL; AY519590; AAS10060.1; -; mRNA.
DR EMBL; AP000371; BAB02538.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77380.1; -; Genomic_DNA.
DR PIR; A39289; TVMUG1.
DR RefSeq; NP_189430.1; NM_113708.2.
DR AlphaFoldDB; P27900; -.
DR SMR; P27900; -.
DR BioGRID; 7745; 10.
DR DIP; DIP-38476N; -.
DR IntAct; P27900; 5.
DR STRING; 3702.AT3G27920.1; -.
DR PaxDb; P27900; -.
DR PRIDE; P27900; -.
DR EnsemblPlants; AT3G27920.1; AT3G27920.1; AT3G27920.
DR GeneID; 822415; -.
DR Gramene; AT3G27920.1; AT3G27920.1; AT3G27920.
DR KEGG; ath:AT3G27920; -.
DR Araport; AT3G27920; -.
DR TAIR; locus:2086475; AT3G27920.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_25_8_1; -.
DR InParanoid; P27900; -.
DR OMA; MMNFASG; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; P27900; -.
DR PRO; PR:P27900; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; P27900; baseline and differential.
DR Genevisible; P27900; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0001708; P:cell fate specification; IMP:TAIR.
DR GO; GO:0010154; P:fruit development; IMP:TAIR.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; TAS:TAIR.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IMP:TAIR.
DR GO; GO:0032880; P:regulation of protein localization; IMP:TAIR.
DR GO; GO:2000039; P:regulation of trichome morphogenesis; IMP:TAIR.
DR GO; GO:0010026; P:trichome differentiation; IMP:TAIR.
DR GO; GO:0048629; P:trichome patterning; IMP:TAIR.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Repeat; Transcription; Transcription regulation.
FT CHAIN 1..228
FT /note="Trichome differentiation protein GL1"
FT /id="PRO_0000197071"
FT DOMAIN 11..63
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 64..118
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 39..63
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 91..114
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT VARIANT 130..132
FT /note="GED -> VEE (in strain: cv. Can-0)"
FT VARIANT 148
FT /note="Missing (in strain: cv. Kas-1, cv. Ms-0)"
FT /evidence="ECO:0000269|PubMed:11504855"
FT VARIANT 149
FT /note="C -> R (in strain: cv. Aa-0, cv. An-1, cv. Bur-0,
FT cv. Can-0, cv. Es-0, cv. Gr-1, cv. Hi-0, cv. Ita-0, cv.
FT Kas-1, cv. Ms-0, cv. Mt-0, cv. Nd-0, cv. No-0, cv. Oy-0,
FT cv. RLD, cv. Tsu-0, cv. Wassilewskija and cv. Yo-0)"
FT /evidence="ECO:0000269|PubMed:11504855"
FT VARIANT 176
FT /note="P -> R (in strain: cv. Di-0 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:11504855"
FT VARIANT 222..224
FT /note="GDV -> SDI (in strain: cv. Aa-0, cv. An-1, cv. Bur-
FT 0, cv. Can-0, cv. Es-0, cv. Gr-1, cv. Hi-0, cv. Ita-0, cv.
FT Kas-1, cv. Ms-0, cv. Mt-0, cv. Nd-0, cv. No-0, cv. Oy-0,
FT cv. RLD, cv. Tsu-0, cv. Wassilewskija and cv. Yo-0)"
FT MUTAGEN 100
FT /note="G->R: In gl1-65; few trichomes on leaf margins,
FT cauline leaves, and stems."
FT /evidence="ECO:0000269|PubMed:11504855"
FT MUTAGEN 182..228
FT /note="Missing: In gl1-323; few trichomes on leaf margins,
FT cauline leaves."
FT /evidence="ECO:0000269|PubMed:8111017"
FT MUTAGEN 202..228
FT /note="Missing: In gl1-2; reduced trichome density,
FT truncated trichomes."
FT /evidence="ECO:0000269|PubMed:8111017"
SQ SEQUENCE 228 AA; 26339 MW; 822A8646F446F6B9 CRC64;
MRIRRRDEKE NQEYKKGLWT VEEDNILMDY VLNHGTGQWN RIVRKTGLKR CGKSCRLRWM
NYLSPNVNKG NFTEQEEDLI IRLHKLLGNR WSLIAKRVPG RTDNQVKNYW NTHLSKKLVG
DYSSAVKTTG EDDDSPPSLF ITAATPSSCH HQQENIYENI AKSFNGVVSA SYEDKPKQEL
AQKDVLMATT NDPSHYYGNN ALWVHDDDFE LSSLVMMNFA SGDVEYCL
