GL21_ARATH
ID GL21_ARATH Reviewed; 219 AA.
AC P94014; F4I108; O80538; P94097;
DT 29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Germin-like protein subfamily 2 member 1;
DE Flags: Precursor;
GN Name=GLP4; Synonyms=GLP5; OrderedLocusNames=At1g09560; ORFNames=F14J9.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=9869400; DOI=10.1023/a:1006038117130;
RA Carter C., Graham R.A., Thornburg R.W.;
RT "Arabidopsis thaliana contains a large family of germin-like proteins:
RT characterization of cDNA and genomic sequences encoding 12 unique family
RT members.";
RL Plant Mol. Biol. 38:929-943(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: May play a role in plant defense. Probably has no oxalate
CC oxidase activity even if the active site is conserved.
CC -!- SUBUNIT: Oligomer (believed to be a pentamer but probably hexamer).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the germin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB51569.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC Sequence=AAB51576.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC Sequence=AAB51577.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC Sequence=AAB51578.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; U75191; AAB51569.1; ALT_SEQ; mRNA.
DR EMBL; U75198; AAB51576.1; ALT_SEQ; mRNA.
DR EMBL; U75199; AAB51577.1; ALT_SEQ; mRNA.
DR EMBL; U75200; AAB51578.1; ALT_SEQ; mRNA.
DR EMBL; AC003970; AAC33216.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28461.1; -; Genomic_DNA.
DR EMBL; AF326875; AAG41457.1; -; mRNA.
DR EMBL; AF324678; AAG40029.1; -; mRNA.
DR EMBL; AF339696; AAK00378.1; -; mRNA.
DR PIR; C86229; C86229.
DR RefSeq; NP_172427.1; NM_100827.4.
DR AlphaFoldDB; P94014; -.
DR SMR; P94014; -.
DR STRING; 3702.AT1G09560.1; -.
DR PaxDb; P94014; -.
DR PRIDE; P94014; -.
DR ProteomicsDB; 247392; -.
DR EnsemblPlants; AT1G09560.1; AT1G09560.1; AT1G09560.
DR GeneID; 837482; -.
DR Gramene; AT1G09560.1; AT1G09560.1; AT1G09560.
DR KEGG; ath:AT1G09560; -.
DR Araport; AT1G09560; -.
DR TAIR; locus:2012260; AT1G09560.
DR eggNOG; ENOG502QQ4A; Eukaryota.
DR HOGENOM; CLU_015790_0_3_1; -.
DR InParanoid; P94014; -.
DR OMA; ARIHYQP; -.
DR OrthoDB; 1164277at2759; -.
DR PRO; PR:P94014; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P94014; baseline and differential.
DR Genevisible; P94014; AT.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; IDA:TAIR.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR GO; GO:0010497; P:plasmodesmata-mediated intercellular transport; IMP:TAIR.
DR GO; GO:2000280; P:regulation of root development; IMP:TAIR.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR006045; Cupin_1.
DR InterPro; IPR001929; Germin.
DR InterPro; IPR019780; Germin_Mn-BS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR Pfam; PF00190; Cupin_1; 1.
DR PRINTS; PR00325; GERMIN.
DR SMART; SM00835; Cupin_1; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
DR PROSITE; PS00725; GERMIN; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Disulfide bond; Glycoprotein; Manganese; Metal-binding;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..219
FT /note="Germin-like protein subfamily 2 member 1"
FT /id="PRO_0000010821"
FT DOMAIN 61..209
FT /note="Cupin type-1"
FT /evidence="ECO:0000255"
FT BINDING 109
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 111
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 116
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 155
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT CARBOHYD 70
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 32..47
FT /evidence="ECO:0000250"
SQ SEQUENCE 219 AA; 22868 MW; A4B659CBB606C968 CRC64;
MASPTLTLLL LLTTVSFFIS SSADPDMLQD LCVADLPSGI KINGFPCKDA ATVTSADFFS
QGLAKPGLTN NTFGALVTGA NVMTIPGLNT LGVSLSRIDY APGGLNPPHT HPRATEVVFV
LEGTLDVGFL TTANKLISQS LKKGDVFAFP KGLVHFQKNN GDVPASVIAA FNSQLPGTQS
LGATLFGSTP PVPDNILAQA FQTSPGTVKH IKSKFQPKK
