ID   GL6D1_MOUSE             Reviewed;         311 AA.
AC   Q2NKH9;
DT   04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Glycosyltransferase 6 domain-containing protein 1;
DE            EC=2.4.1.-;
GN   Name=Glt6d1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000250|UniProtKB:P14769};
CC       Note=Binds 1 Mn(2+) ion per subunit. {ECO:0000250|UniProtKB:P14769};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 6 family. {ECO:0000305}.
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DR   EMBL; AL731682; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BX649340; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC111823; AAI11824.1; -; mRNA.
DR   CCDS; CCDS15791.1; -.
DR   RefSeq; NP_001034184.1; NM_001039095.1.
DR   AlphaFoldDB; Q2NKH9; -.
DR   SMR; Q2NKH9; -.
DR   STRING; 10090.ENSMUSP00000048642; -.
DR   CAZy; GT6; Glycosyltransferase Family 6.
DR   GlyGen; Q2NKH9; 1 site.
DR   iPTMnet; Q2NKH9; -.
DR   PhosphoSitePlus; Q2NKH9; -.
DR   MaxQB; Q2NKH9; -.
DR   PaxDb; Q2NKH9; -.
DR   PRIDE; Q2NKH9; -.
DR   ProteomicsDB; 263361; -.
DR   Antibodypedia; 8276; 54 antibodies from 16 providers.
DR   Ensembl; ENSMUST00000038010; ENSMUSP00000048642; ENSMUSG00000036401.
DR   GeneID; 71103; -.
DR   KEGG; mmu:71103; -.
DR   UCSC; uc008itl.1; mouse.
DR   CTD; 360203; -.
DR   MGI; MGI:1918353; Glt6d1.
DR   VEuPathDB; HostDB:ENSMUSG00000036401; -.
DR   eggNOG; ENOG502RU0J; Eukaryota.
DR   GeneTree; ENSGT00950000182858; -.
DR   HOGENOM; CLU_062445_1_0_1; -.
DR   InParanoid; Q2NKH9; -.
DR   OMA; WLAPILW; -.
DR   OrthoDB; 1204439at2759; -.
DR   PhylomeDB; Q2NKH9; -.
DR   TreeFam; TF330991; -.
DR   BioGRID-ORCS; 71103; 4 hits in 73 CRISPR screens.
DR   PRO; PR:Q2NKH9; -.
DR   Proteomes; UP000000589; Chromosome 2.
DR   RNAct; Q2NKH9; protein.
DR   Bgee; ENSMUSG00000036401; Expressed in seminiferous tubule of testis and 3 other tissues.
DR   Genevisible; Q2NKH9; MM.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031982; C:vesicle; IBA:GO_Central.
DR   GO; GO:0016758; F:hexosyltransferase activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0030259; P:lipid glycosylation; IBA:GO_Central.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR005076; Glyco_trans_6.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   PANTHER; PTHR10462; PTHR10462; 1.
DR   Pfam; PF03414; Glyco_transf_6; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Glycosyltransferase; Membrane; Reference proteome;
KW   Signal-anchor; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..311
FT                   /note="Glycosyltransferase 6 domain-containing protein 1"
FT                   /id="PRO_0000311973"
FT   TOPO_DOM        1..5
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        6..26
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        27..311
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        266
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:P14769"
FT   BINDING         85..90
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P14769"
FT   BINDING         176..178
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P14769"
FT   BINDING         198..201
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P14769"
FT   CARBOHYD        77
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   311 AA;  37081 MW;  D13B59C51C2552E3 CRC64;
     MKAKRRILLQ LLTFCLFLLL LAKIHFRNHQ EEELLLSDWF NPRRRLDVIT TTDWLAPIIW
     EGTFDRKVLE TYFRKQNITV GLAVFAVGSL TDKYLDPFLQ SASKFFMPGY RVIFYIMVDK
     YLQLQEMEHN PLQSFQVLKI GKERWWSNFD LMRMKILSEH IRDHIRYEVD FLFSMNINMV
     FQSEFGVETL STSVAQLHPW WYFRKRTNLP YERKPTSVAH IPFGLGDFYY AGAIIGGVPF
     QVLDFAQQYL KGVFLDTENG VNSTYEKYLN KYFFLNKPTK LLSPEYSWDP TFNLPRHVWF
     VKIAHHPIDT L