GL814_ORYSJ
ID GL814_ORYSJ Reviewed; 213 AA.
AC Q6ZBZ2; A0A0P0XGL0; O49001; O65044; Q9SAZ9;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Germin-like protein 8-14;
DE AltName: Full=Germin-like protein 1;
DE AltName: Full=Germin-like protein 5;
DE Short=OsGER5;
DE Flags: Precursor;
GN Name=GER5; Synonyms=GLP1, GLP110;
GN OrderedLocusNames=Os08g0460000, LOC_Os08g35760;
GN ORFNames=OsJ_026469, P0493A04.40, P0690E03.8;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare;
RA Membre N., Bernier F.;
RT "The rice genome expresses at least six different genes for oxalate
RT oxidase/germin-like proteins.";
RL (er) Plant Gene Register PGR98-021(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Ilpoombyeo;
RA Yoon U.H., Hahn J.H., Yun C.-H., Eun M.-Y.;
RT "Molecular cloning and characterization of a cDNA encoding germin-like
RT protein in rice.";
RL Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Akitakomachi;
RA Yoshizawa C., Ono M., Inoue M.;
RT "Molecular cloning and characterization of a gene encoding germin-like
RT protein in rice.";
RL Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [9]
RP PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 24-30, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC STRAIN=cv. Nipponbare;
RX PubMed=16758443; DOI=10.1002/pmic.200600043;
RA Nozu Y., Tsugita A., Kamijo K.;
RT "Proteomic analysis of rice leaf, stem and root tissues during growth
RT course.";
RL Proteomics 6:3665-3670(2006).
RN [10]
RP INDUCTION, PHOSPHORYLATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=19103168; DOI=10.1016/j.bbrc.2008.12.067;
RA Ke Y., Han G., He H., Li J.;
RT "Differential regulation of proteins and phosphoproteins in rice under
RT drought stress.";
RL Biochem. Biophys. Res. Commun. 379:133-138(2009).
CC -!- FUNCTION: May play a role in plant defense. Probably has no oxalate
CC oxidase activity even if the active site is conserved.
CC -!- SUBUNIT: Oligomer (believed to be a pentamer but probably hexamer).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250}.
CC -!- INDUCTION: Down-regulated by drought stress.
CC {ECO:0000269|PubMed:19103168}.
CC -!- PTM: Phosphorylated on threonine residue.
CC {ECO:0000269|PubMed:19103168}.
CC -!- SIMILARITY: Belongs to the germin family. {ECO:0000305}.
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DR EMBL; AF032975; AAC04836.1; -; mRNA.
DR EMBL; AF051156; AAC05682.1; -; mRNA.
DR EMBL; AB010876; BAA74702.1; -; mRNA.
DR EMBL; AB015593; BAB17848.1; -; Genomic_DNA.
DR EMBL; AP004586; BAD09603.1; -; Genomic_DNA.
DR EMBL; AP004707; BAD09958.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF23890.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT05757.1; -; Genomic_DNA.
DR EMBL; CM000145; EAZ42986.1; -; Genomic_DNA.
DR EMBL; AK065284; BAG89446.1; -; mRNA.
DR EMBL; AK104729; BAG96911.1; -; mRNA.
DR PIR; T02871; T02871.
DR RefSeq; XP_015648639.1; XM_015793153.1.
DR AlphaFoldDB; Q6ZBZ2; -.
DR SMR; Q6ZBZ2; -.
DR STRING; 4530.OS08T0460000-01; -.
DR PaxDb; Q6ZBZ2; -.
DR PRIDE; Q6ZBZ2; -.
DR EnsemblPlants; Os08t0460000-01; Os08t0460000-01; Os08g0460000.
DR GeneID; 4345763; -.
DR Gramene; Os08t0460000-01; Os08t0460000-01; Os08g0460000.
DR KEGG; osa:4345763; -.
DR eggNOG; ENOG502QT7C; Eukaryota.
DR HOGENOM; CLU_015790_0_2_1; -.
DR InParanoid; Q6ZBZ2; -.
DR OMA; KMIIQIF; -.
DR OrthoDB; 1164277at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000007752; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR Genevisible; Q6ZBZ2; OS.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR006045; Cupin_1.
DR InterPro; IPR001929; Germin.
DR InterPro; IPR019780; Germin_Mn-BS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR Pfam; PF00190; Cupin_1; 1.
DR PRINTS; PR00325; GERMIN.
DR SMART; SM00835; Cupin_1; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
DR PROSITE; PS00725; GERMIN; 1.
PE 1: Evidence at protein level;
KW Apoplast; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW Manganese; Metal-binding; Phosphoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:16758443"
FT CHAIN 24..213
FT /note="Germin-like protein 8-14"
FT /id="PRO_0000365526"
FT DOMAIN 56..203
FT /note="Cupin type-1"
FT /evidence="ECO:0000255"
FT BINDING 104
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 106
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 111
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 151
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT CARBOHYD 63
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 29..44
FT /evidence="ECO:0000250"
FT CONFLICT 92
FT /note="R -> K (in Ref. 2; AAC05682)"
FT /evidence="ECO:0000305"
FT CONFLICT 166
FT /note="A -> G (in Ref. 3; BAA74702)"
FT /evidence="ECO:0000305"
FT CONFLICT 177
FT /note="T -> N (in Ref. 3; BAA74702)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 213 AA; 21861 MW; F6B49974C3ADC2CC CRC64;
MAKAVMMLPV LLSFLLLPFS SMALTQDFCV ADLTCSDTPA GYPCKASVGA GDFAYHGLAA
AGNTSNLIKA AVTPAFVGQF PGVNGLGISA ARLDIAVGGV VPLHTHPAAS ELLFVTQGTV
AAGFITSSSN TVYTRTLYAG DIMVFPQGLL HYQYNAGQSA AVALVAFSGP NPGLQITDYA
LFANNLPSAI VEKVTFLDDA QVKKLKSVLG GSG
