GL82_ORYSJ
ID GL82_ORYSJ Reviewed; 221 AA.
AC Q6YZA9; A0A0P0XCM3; B1NEV1; O48999; O49181;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Germin-like protein 8-2;
DE AltName: Full=Germin-like protein 16;
DE AltName: Full=Germin-like protein 3;
DE Short=OsGER3;
DE Flags: Precursor;
GN Name=GER3; Synonyms=GLP16; OrderedLocusNames=Os08g0189100, LOC_Os08g08960;
GN ORFNames=B1099H05.23, OsJ_025236;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare;
RA Yun C.-H., Park J.-H., Lee J.-H., Eun M.-Y.;
RT "Nucleotide sequence of rice germin-like protein.";
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Ilpoombyeo;
RA Yoon U.H., Kim Y.H.;
RT "Molecular cloning of germin like protein genes in rice seeds.";
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=19027836; DOI=10.1016/j.gene.2008.10.017;
RA Cheng X., Liu G., Ye G., Wang H., Shen X., Wu K., Xie J., Altosaar I.;
RT "Screening and cloning of antimicrobial DNA sequences using a vital
RT staining method.";
RL Gene 430:132-139(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [9]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 41-221.
RC STRAIN=cv. Nipponbare;
RA Membre N., Bernier F.;
RT "The rice genome expresses at least six different genes for oxalate
RT oxidase/germin-like proteins.";
RL (er) Plant Gene Register PGR98-021(1998).
RN [10]
RP FUNCTION.
RX PubMed=19011003; DOI=10.1104/pp.108.128348;
RA Manosalva P.M., Davidson R.M., Liu B., Zhu X., Hulbert S.H., Leung H.,
RA Leach J.E.;
RT "A germin-like protein gene family functions as a complex quantitative
RT trait locus conferring broad-spectrum disease resistance in rice.";
RL Plant Physiol. 149:286-296(2009).
CC -!- FUNCTION: Plays role in broad-spectrum disease resistance. Probably has
CC no oxalate oxidase activity even if the active site is conserved.
CC {ECO:0000269|PubMed:19011003}.
CC -!- SUBUNIT: Oligomer (believed to be a pentamer but probably hexamer).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: Member of the 12 germin-like protein gene cluster
CC located on chromosome 8 in the major-effect quantitative trait loci
CC (QTL) for fungal blast resistance. Partial suppression of the 12
CC germin-like protein genes increases susceptibility to the fungal
CC pathogens causing rice blast and sheath blight diseases.
CC -!- SIMILARITY: Belongs to the germin family. {ECO:0000305}.
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DR EMBL; AF042489; AAB97470.1; -; mRNA.
DR EMBL; EF122484; ABL74571.1; -; mRNA.
DR EMBL; EF444528; ABS44860.1; -; mRNA.
DR EMBL; AP005531; BAD05768.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF23070.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT04149.1; -; Genomic_DNA.
DR EMBL; CM000145; EAZ41753.1; -; Genomic_DNA.
DR EMBL; AK062862; BAG88472.1; -; mRNA.
DR EMBL; AF032973; AAC04834.1; -; mRNA.
DR PIR; T02591; T02591.
DR PIR; T02666; T02666.
DR RefSeq; XP_015648581.1; XM_015793095.1.
DR AlphaFoldDB; Q6YZA9; -.
DR SMR; Q6YZA9; -.
DR STRING; 4530.OS08T0189100-01; -.
DR PaxDb; Q6YZA9; -.
DR PRIDE; Q6YZA9; -.
DR EnsemblPlants; Os08t0189100-01; Os08t0189100-01; Os08g0189100.
DR GeneID; 4344838; -.
DR Gramene; Os08t0189100-01; Os08t0189100-01; Os08g0189100.
DR KEGG; osa:4344838; -.
DR eggNOG; ENOG502QQ4A; Eukaryota.
DR HOGENOM; CLU_015790_0_0_1; -.
DR InParanoid; Q6YZA9; -.
DR OMA; CVAINKP; -.
DR OrthoDB; 1164277at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000007752; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR Genevisible; Q6YZA9; OS.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR006045; Cupin_1.
DR InterPro; IPR001929; Germin.
DR InterPro; IPR019780; Germin_Mn-BS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR Pfam; PF00190; Cupin_1; 1.
DR PRINTS; PR00325; GERMIN.
DR SMART; SM00835; Cupin_1; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
DR PROSITE; PS00725; GERMIN; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Disulfide bond; Glycoprotein; Manganese; Metal-binding;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..221
FT /note="Germin-like protein 8-2"
FT /id="PRO_0000365514"
FT DOMAIN 64..215
FT /note="Cupin type-1"
FT /evidence="ECO:0000255"
FT BINDING 112
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 114
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 119
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 160
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT CARBOHYD 54
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 79
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 34..49
FT /evidence="ECO:0000250"
FT CONFLICT 59
FT /note="D -> N (in Ref. 9; AAC04834)"
FT /evidence="ECO:0000305"
FT CONFLICT 72
FT /note="T -> A (in Ref. 1; AAB97470)"
FT /evidence="ECO:0000305"
FT CONFLICT 131
FT /note="G -> S (in Ref. 3; ABS44860)"
FT /evidence="ECO:0000305"
FT CONFLICT 134
FT /note="T -> M (in Ref. 1; AAB97470)"
FT /evidence="ECO:0000305"
FT CONFLICT 158
FT /note="L -> F (in Ref. 1; AAB97470)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 221 AA; 23712 MW; D8D43E7D167C0FCD CRC64;
MASSSFSFLL VAALLGLASW KAIASDPSPL QDFCVADLNS PVRVNGFVCK NPMNASADDF
FKAAMLDKPR DTNNKVGSNV TLVNVLQLPG LNTLGISIAR LDFAPLGLNP PHTHPRATEI
FTVLEGTLYV GFVTSNPDNR LLSKVLNKGD VFVFPEGLIH FQFNPNPHKP AVAIAALSSQ
NPGVITIANA VFGSNPPISD DILMKAFQVD KKIIDLLQAQ F
