GL83_ORYSJ
ID GL83_ORYSJ Reviewed; 225 AA.
AC Q6YZZ7; A0A0P0XCI3; O48998;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Germin-like protein 8-3;
DE AltName: Full=Germin-like protein 2;
DE Short=OsGER2;
DE Flags: Precursor;
GN Name=GER2; OrderedLocusNames=Os08g0189200, LOC_Os08g08970;
GN ORFNames=B1099H05.26, OsJ_025237, P0610E02.2;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare;
RA Membre N., Bernier F.;
RT "The rice genome expresses at least six different genes for oxalate
RT oxidase/germin-like proteins.";
RL (er) Plant Gene Register PGR98-021(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP FUNCTION.
RX PubMed=19011003; DOI=10.1104/pp.108.128348;
RA Manosalva P.M., Davidson R.M., Liu B., Zhu X., Hulbert S.H., Leung H.,
RA Leach J.E.;
RT "A germin-like protein gene family functions as a complex quantitative
RT trait locus conferring broad-spectrum disease resistance in rice.";
RL Plant Physiol. 149:286-296(2009).
CC -!- FUNCTION: Plays role in broad-spectrum disease resistance. Probably has
CC no oxalate oxidase activity even if the active site is conserved.
CC {ECO:0000269|PubMed:19011003}.
CC -!- SUBUNIT: Oligomer (believed to be a pentamer but probably hexamer).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: Member of the 12 germin-like protein gene cluster
CC located on chromosome 8 in the major-effect quantitative trait loci
CC (QTL) for fungal blast resistance. Partial suppression of the 12
CC germin-like protein genes increases susceptibility to the fungal
CC pathogens causing rice blast and sheath blight diseases.
CC -!- SIMILARITY: Belongs to the germin family. {ECO:0000305}.
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DR EMBL; AF032972; AAC04833.1; -; mRNA.
DR EMBL; AP005505; BAD05730.1; -; Genomic_DNA.
DR EMBL; AP005531; BAD05769.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF23071.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT04150.1; -; Genomic_DNA.
DR EMBL; CM000145; EAZ41754.1; -; Genomic_DNA.
DR EMBL; AK059781; BAG87123.1; -; mRNA.
DR PIR; T02241; T02241.
DR RefSeq; XP_015650201.1; XM_015794715.1.
DR AlphaFoldDB; Q6YZZ7; -.
DR SMR; Q6YZZ7; -.
DR STRING; 4530.OS08T0189200-01; -.
DR PaxDb; Q6YZZ7; -.
DR PRIDE; Q6YZZ7; -.
DR EnsemblPlants; Os08t0189200-01; Os08t0189200-01; Os08g0189200.
DR GeneID; 4344839; -.
DR Gramene; Os08t0189200-01; Os08t0189200-01; Os08g0189200.
DR KEGG; osa:4344839; -.
DR eggNOG; ENOG502QQ4A; Eukaryota.
DR HOGENOM; CLU_015790_0_0_1; -.
DR InParanoid; Q6YZZ7; -.
DR OMA; NPAMIIG; -.
DR OrthoDB; 1164277at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000007752; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR Genevisible; Q6YZZ7; OS.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR006045; Cupin_1.
DR InterPro; IPR001929; Germin.
DR InterPro; IPR019780; Germin_Mn-BS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR Pfam; PF00190; Cupin_1; 1.
DR PRINTS; PR00325; GERMIN.
DR SMART; SM00835; Cupin_1; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
DR PROSITE; PS00725; GERMIN; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Disulfide bond; Glycoprotein; Manganese; Metal-binding;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..225
FT /note="Germin-like protein 8-3"
FT /id="PRO_0000365515"
FT DOMAIN 60..213
FT /note="Cupin type-1"
FT /evidence="ECO:0000255"
FT BINDING 111
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 113
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 118
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 158
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT CARBOHYD 53
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 78
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 33..48
FT /evidence="ECO:0000250"
SQ SEQUENCE 225 AA; 24594 MW; 0145C3B87B9C5E54 CRC64;
MASSSLFLLA SLLVLASWQQ AIAFDPSPLQ DFCVADMASP VRVNGFPCKN PMNVTSDDFF
NAAKFDMPRN TMNKVGSNVT NLNVINFPGL NTLGISLARI DYAPMGVNPP HVHPRATELL
TVLEGTLYVG FVTSNPNRLF SKVVHKGDVF VFPKAMIHFQ MNLDHNKPAV AQSALSSQNP
GVITIASAIF GSTPPISDDV LVKAFQVEKK VIDWLKSQFS ENNHY
