ID   GL88_ORYSJ              Reviewed;         224 AA.
AC   Q6YZA1; A0A0P0XCG6; Q0J7I9;
DT   03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Germin-like protein 8-8;
DE   Flags: Precursor;
GN   OrderedLocusNames=Os08g0189700, LOC_Os08g09020;
GN   ORFNames=B1099H05.34, OsJ_025247, P0610E02.10;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RG   The rice full-length cDNA consortium;
RT   "Oryza sativa full length cDNA.";
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   FUNCTION.
RX   PubMed=19011003; DOI=10.1104/pp.108.128348;
RA   Manosalva P.M., Davidson R.M., Liu B., Zhu X., Hulbert S.H., Leung H.,
RA   Leach J.E.;
RT   "A germin-like protein gene family functions as a complex quantitative
RT   trait locus conferring broad-spectrum disease resistance in rice.";
RL   Plant Physiol. 149:286-296(2009).
CC   -!- FUNCTION: Plays role in broad-spectrum disease resistance. Probably has
CC       no oxalate oxidase activity even if the active site is conserved.
CC       {ECO:0000269|PubMed:19011003}.
CC   -!- SUBUNIT: Oligomer (believed to be a pentamer but probably hexamer).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000250}.
CC   -!- MISCELLANEOUS: Member of the 12 germin-like protein gene cluster
CC       located on chromosome 8 in the major-effect quantitative trait loci
CC       (QTL) for fungal blast resistance. Partial suppression of the 12
CC       germin-like protein genes increases susceptibility to the fungal
CC       pathogens causing rice blast and sheath blight diseases.
CC   -!- SIMILARITY: Belongs to the germin family. {ECO:0000305}.
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DR   EMBL; AP005505; BAD05737.1; -; Genomic_DNA.
DR   EMBL; AP005531; BAD05776.1; -; Genomic_DNA.
DR   EMBL; AP008214; BAF23076.2; -; Genomic_DNA.
DR   EMBL; AP014964; BAT04155.1; -; Genomic_DNA.
DR   EMBL; CM000145; EAZ41764.1; -; Genomic_DNA.
DR   EMBL; AK241043; BAH00937.1; -; mRNA.
DR   RefSeq; XP_015648995.1; XM_015793509.1.
DR   AlphaFoldDB; Q6YZA1; -.
DR   SMR; Q6YZA1; -.
DR   PaxDb; Q6YZA1; -.
DR   PRIDE; Q6YZA1; -.
DR   EnsemblPlants; Os08t0189700-01; Os08t0189700-01; Os08g0189700.
DR   GeneID; 4344844; -.
DR   Gramene; Os08t0189700-01; Os08t0189700-01; Os08g0189700.
DR   KEGG; osa:4344844; -.
DR   eggNOG; ENOG502QQ4A; Eukaryota.
DR   HOGENOM; CLU_015790_0_0_1; -.
DR   InParanoid; Q6YZA1; -.
DR   OMA; HFQLSIV; -.
DR   OrthoDB; 1164277at2759; -.
DR   Proteomes; UP000000763; Chromosome 8.
DR   Proteomes; UP000007752; Chromosome 8.
DR   Proteomes; UP000059680; Chromosome 8.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR001929; Germin.
DR   InterPro; IPR019780; Germin_Mn-BS.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 1.
DR   PRINTS; PR00325; GERMIN.
DR   SMART; SM00835; Cupin_1; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   PROSITE; PS00725; GERMIN; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Disulfide bond; Glycoprotein; Manganese; Metal-binding;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..224
FT                   /note="Germin-like protein 8-8"
FT                   /id="PRO_0000365520"
FT   DOMAIN          62..212
FT                   /note="Cupin type-1"
FT                   /evidence="ECO:0000255"
FT   BINDING         109
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         111
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         116
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         157
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        76
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        135
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        32..47
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   224 AA;  24250 MW;  95480D9E6C32E127 CRC64;
     MASPSFCLLA ALLALVSWQA IASDPSPLQD FCVADKHSPV LVNGFACLDP KYVTADHFFK
     AAMLDTPRKT NKVGSNVTLI NVMQIPGLNT LGISIARIDY APLGENPPHT HPRATEILTV
     LEGTLYVGFV TSNPNNTLFS KVLNKGDVFV FPEGLIHFQF NPNPHQPAVA IAALSSQNPG
     AITIANAVFG SKPPISDKVL AKAFQVEKGT IDWLQAQFWE NNHY