GL89_ORYSJ
ID GL89_ORYSJ Reviewed; 224 AA.
AC Q6YZ99; C7J647;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Germin-like protein 8-9;
DE Flags: Precursor;
GN OrderedLocusNames=Os08g0189850, LOC_Os08g09040;
GN ORFNames=B1099H05.37, OsJ_025249, P0610E02.13;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP FUNCTION.
RX PubMed=19011003; DOI=10.1104/pp.108.128348;
RA Manosalva P.M., Davidson R.M., Liu B., Zhu X., Hulbert S.H., Leung H.,
RA Leach J.E.;
RT "A germin-like protein gene family functions as a complex quantitative
RT trait locus conferring broad-spectrum disease resistance in rice.";
RL Plant Physiol. 149:286-296(2009).
CC -!- FUNCTION: Plays role in broad-spectrum disease resistance. Probably has
CC no oxalate oxidase activity even if the active site is conserved.
CC {ECO:0000269|PubMed:19011003}.
CC -!- SUBUNIT: Oligomer (believed to be a pentamer but probably hexamer).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: Member of the 12 germin-like protein gene cluster
CC located on chromosome 8 in the major-effect quantitative trait loci
CC (QTL) for fungal blast resistance. Partial suppression of the 12
CC germin-like protein genes increases susceptibility to the fungal
CC pathogens causing rice blast and sheath blight diseases.
CC -!- SIMILARITY: Belongs to the germin family. {ECO:0000305}.
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DR EMBL; AP005505; BAD05739.1; -; Genomic_DNA.
DR EMBL; AP005531; BAD05778.1; -; Genomic_DNA.
DR EMBL; AP008214; BAH94143.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT04158.1; -; Genomic_DNA.
DR EMBL; CM000145; EAZ41766.1; -; Genomic_DNA.
DR RefSeq; XP_015648901.1; XM_015793415.1.
DR AlphaFoldDB; Q6YZ99; -.
DR SMR; Q6YZ99; -.
DR STRING; 4530.OS08T0189850-00; -.
DR PaxDb; Q6YZ99; -.
DR PRIDE; Q6YZ99; -.
DR EnsemblPlants; Os08t0189850-01; Os08t0189850-01; Os08g0189850.
DR GeneID; 9272376; -.
DR Gramene; Os08t0189850-01; Os08t0189850-01; Os08g0189850.
DR KEGG; osa:9272376; -.
DR HOGENOM; CLU_015790_0_0_1; -.
DR InParanoid; Q6YZ99; -.
DR OMA; SLAHIDF; -.
DR OrthoDB; 1164277at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000007752; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR006045; Cupin_1.
DR InterPro; IPR001929; Germin.
DR InterPro; IPR019780; Germin_Mn-BS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR Pfam; PF00190; Cupin_1; 1.
DR PRINTS; PR00325; GERMIN.
DR SMART; SM00835; Cupin_1; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
DR PROSITE; PS00725; GERMIN; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Disulfide bond; Glycoprotein; Manganese; Metal-binding;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..224
FT /note="Germin-like protein 8-9"
FT /id="PRO_0000365521"
FT DOMAIN 62..212
FT /note="Cupin type-1"
FT /evidence="ECO:0000255"
FT BINDING 109
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 111
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 116
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 157
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT CARBOHYD 76
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 135
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 32..47
FT /evidence="ECO:0000250"
SQ SEQUENCE 224 AA; 24184 MW; 288B5344B0BC2B3D CRC64;
MASPSFCLFA ALLALVSWQA IASDPSPLQD FCVADKHSPV LVNGFACLDP KYVTADHFFK
AAMLDTPRKT NKVGSNVTLI NVMQIPGLNT LGISIARIDY APLGQNPPHT HPRATEILTV
LEGTLHVGFV TSNPNNTLFS KVLNKGDVFV FPVGLIHFQF NPNPHQPAVA IAALSSQNPG
VITIANAVFG SKPPISDEVL AKAFQVGKGT IDWLQAQFWE NNHY
