GL8D1_MOUSE
ID GL8D1_MOUSE Reviewed; 371 AA.
AC Q6NSU3; Q3TV18; Q9CWT8;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Glycosyltransferase 8 domain-containing protein 1;
DE EC=2.4.1.-;
GN Name=Glt8d1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6NSU3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6NSU3-2; Sequence=VSP_025707, VSP_025708;
CC -!- SIMILARITY: Belongs to the glycosyltransferase 8 family. {ECO:0000305}.
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DR EMBL; AK010388; BAB26903.1; -; mRNA.
DR EMBL; AK160461; BAE35802.1; -; mRNA.
DR EMBL; BC069873; AAH69873.1; -; mRNA.
DR CCDS; CCDS26904.1; -. [Q6NSU3-1]
DR RefSeq; NP_001159402.1; NM_001165930.1. [Q6NSU3-1]
DR RefSeq; NP_083902.2; NM_029626.2. [Q6NSU3-1]
DR RefSeq; XP_006519732.2; XM_006519669.2.
DR RefSeq; XP_006519735.1; XM_006519672.3. [Q6NSU3-1]
DR RefSeq; XP_006519736.1; XM_006519673.3. [Q6NSU3-1]
DR RefSeq; XP_006519737.1; XM_006519674.2. [Q6NSU3-1]
DR AlphaFoldDB; Q6NSU3; -.
DR SMR; Q6NSU3; -.
DR BioGRID; 218149; 1.
DR STRING; 10090.ENSMUSP00000022476; -.
DR CAZy; GT8; Glycosyltransferase Family 8.
DR GlyGen; Q6NSU3; 1 site.
DR PhosphoSitePlus; Q6NSU3; -.
DR EPD; Q6NSU3; -.
DR MaxQB; Q6NSU3; -.
DR PaxDb; Q6NSU3; -.
DR PeptideAtlas; Q6NSU3; -.
DR PRIDE; Q6NSU3; -.
DR ProteomicsDB; 268882; -. [Q6NSU3-1]
DR ProteomicsDB; 268883; -. [Q6NSU3-2]
DR Antibodypedia; 2243; 163 antibodies from 19 providers.
DR DNASU; 76485; -.
DR Ensembl; ENSMUST00000022476; ENSMUSP00000022476; ENSMUSG00000021916. [Q6NSU3-1]
DR Ensembl; ENSMUST00000168584; ENSMUSP00000129323; ENSMUSG00000021916. [Q6NSU3-1]
DR GeneID; 76485; -.
DR KEGG; mmu:76485; -.
DR UCSC; uc007swf.2; mouse. [Q6NSU3-1]
DR UCSC; uc007swg.2; mouse. [Q6NSU3-2]
DR CTD; 55830; -.
DR MGI; MGI:1923735; Glt8d1.
DR VEuPathDB; HostDB:ENSMUSG00000021916; -.
DR eggNOG; ENOG502QTN8; Eukaryota.
DR GeneTree; ENSGT00940000159273; -.
DR HOGENOM; CLU_010770_0_0_1; -.
DR InParanoid; Q6NSU3; -.
DR OMA; ARFYLPM; -.
DR OrthoDB; 1520602at2759; -.
DR PhylomeDB; Q6NSU3; -.
DR TreeFam; TF332433; -.
DR BioGRID-ORCS; 76485; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Glt8d1; mouse.
DR PRO; PR:Q6NSU3; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q6NSU3; protein.
DR Bgee; ENSMUSG00000021916; Expressed in otolith organ and 227 other tissues.
DR ExpressionAtlas; Q6NSU3; baseline and differential.
DR Genevisible; Q6NSU3; MM.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR002495; Glyco_trans_8.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF01501; Glyco_transf_8; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Glycoprotein; Glycosyltransferase; Membrane;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..371
FT /note="Glycosyltransferase 8 domain-containing protein 1"
FT /id="PRO_0000288529"
FT TOPO_DOM 1..7
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 8..28
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29..371
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 257
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 216..227
FT /note="YNYIGYLDYKKE -> VMTYCRSHSGKA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_025707"
FT VAR_SEQ 228..371
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_025708"
FT CONFLICT 92
FT /note="R -> G (in Ref. 1; BAB26903)"
FT /evidence="ECO:0000305"
FT CONFLICT 185
FT /note="N -> K (in Ref. 1; BAB26903)"
FT /evidence="ECO:0000305"
FT CONFLICT 363
FT /note="R -> S (in Ref. 1; BAB26903)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 371 AA; 41990 MW; 61E507E46C18185B CRC64;
MSFRKVNIII WVLAVVLFLL VLHHNFLSLS SLLKNDISDS GIVGLQPIDF VASAHQHPVS
ERQEEIPVVI AASEDRLGGT IAAINSVHQN TRSNVMFYIV TFNSTADHLR SWLNSGSLKS
IRYKIVNFDT KLLEGKVKQD PDQGESMKPL TFARFYLPIL VPSAKKAIYM DDDVIVQGDI
LALYNTPLKP GHAAAFSEDC DSASTKVIIR GAGNQYNYIG YLDYKKERIR KLSMKASTCS
FNPGVFVANL TEWKRQNVTN QLEKWMKLNV EEGLYSRTLA GSITTPPLLI VFYQQHSTID
PMWNVRHLGS SAGKRYSPQF VKAAKLLHWN GHFKPWGRTA SYADVWEKWY IPDPTGKFSL
IRRHMDTSNI K
