ID   GLAA_AKKM8              Reviewed;         596 AA.
AC   B2UL12;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=Alpha-1,3-galactosidase A;
DE            EC=3.2.1.n1;
DE   AltName: Full=Exo-alpha-galactosidase A;
DE            EC=3.2.1.22;
DE   Flags: Precursor;
GN   Name=glaA; OrderedLocusNames=Amuc_1463;
OS   Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC
OS   81048 / CCUG 64013 / CIP 107961 / Muc).
OC   Bacteria; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales;
OC   Akkermansiaceae; Akkermansia.
OX   NCBI_TaxID=349741;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP
RC   107961 / Muc;
RX   PubMed=21390229; DOI=10.1371/journal.pone.0016876;
RA   van Passel M.W., Kant R., Zoetendal E.G., Plugge C.M., Derrien M.,
RA   Malfatti S.A., Chain P.S., Woyke T., Palva A., de Vos W.M., Smidt H.;
RT   "The genome of Akkermansia muciniphila, a dedicated intestinal mucin
RT   degrader, and its use in exploring intestinal metagenomes.";
RL   PLoS ONE 6:E16876-E16876(2011).
CC   -!- FUNCTION: Alpha-galactosidase that specifically removes branched alpha-
CC       1,3-linked galactose residues present in blood group B antigens. Has no
CC       activity toward linear alpha-1,3-linked galactose residues (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-
CC         galactosidic residues, producing free D-galactose.; EC=3.2.1.n1;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC         residues in alpha-D-galactosides, including galactose
CC         oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 110 family. A subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CP001071; ACD05285.1; -; Genomic_DNA.
DR   AlphaFoldDB; B2UL12; -.
DR   SMR; B2UL12; -.
DR   STRING; 349741.Amuc_1463; -.
DR   CAZy; GH110; Glycoside Hydrolase Family 110.
DR   EnsemblBacteria; ACD05285; ACD05285; Amuc_1463.
DR   KEGG; amu:Amuc_1463; -.
DR   eggNOG; COG5434; Bacteria.
DR   HOGENOM; CLU_017693_0_0_0; -.
DR   OMA; HYMHGLG; -.
DR   Proteomes; UP000001031; Chromosome.
DR   GO; GO:0052692; F:raffinose alpha-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.160.20.10; -; 3.
DR   InterPro; IPR039448; Beta_helix.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   Pfam; PF13229; Beta_helix; 1.
DR   SUPFAM; SSF51126; SSF51126; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase; Reference proteome; Repeat; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..596
FT                   /note="Alpha-1,3-galactosidase A"
FT                   /id="PRO_5000370228"
FT   REPEAT          351..373
FT                   /note="PbH1 1"
FT   REPEAT          482..504
FT                   /note="PbH1 2"
FT   REPEAT          515..537
FT                   /note="PbH1 3"
FT   REPEAT          547..569
FT                   /note="PbH1 4"
FT   REGION          23..57
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   596 AA;  65952 MW;  FF5E844064C2FADD CRC64;
     MQNPVASLLF ILAMLTGPCP AADYPERTER TQSAGNHVWH IDPDKGNDGN PGTAPSTAWK
     SMAPANRLIM ARGDTLVIHP GEHAVSLALM GEGSKQAPVT IRFMPGRHIF KHGALMTGKP
     QISNTNDAPN EPKAMAIRLM EAKNIRLEGK PGATDILLEG KAIFVCMEHA ENVSLNGLGF
     DYLHPTMGEF LVTEVEGDTM KATIPDGTLY TVKDGNLTWH GPGWEFRMGG YSKVFDSASG
     TFQGRFDPGK TVIRELSPGK ISITFKEGSP TMKPGQSYQN RNTRRDCCGF FQYRSKNILW
     NNCHIYYMHG MGVVSQFCEN IMFSHLKIAP RPRSLRTNSS WADNLHFSGC RGKIIVKDCV
     LGASHDDAVN VHGTHLRIID RPAPNKITVR FMHPQTFGFD AFAAGDRIDY VSCNTLVPYA
     SNTVSGVKQL NEKEIELTLQ HPNPGNIQPD DVVENVTWTP SVHISNTVCR HIPTRGFLLT
     TRKPVLVERC RFEKTGMPAI LVEDDASGWY ESGVVRNMTI SRNTFIQCGE AVIQIVPHAP
     RPEGDVHRNI TITGNTFDLK NGTAIRIRHT GDVKAEKNTF TKDGKKIPEE KAVDIR