ID   GLAA_SHEWM              Reviewed;         608 AA.
AC   B1KD83;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Alpha-1,3-galactosidase A;
DE            EC=3.2.1.n1;
DE   AltName: Full=Exo-alpha-galactosidase A;
DE            EC=3.2.1.22;
DE   Flags: Precursor;
GN   Name=glaA; OrderedLocusNames=Swoo_3656;
OS   Shewanella woodyi (strain ATCC 51908 / MS32).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=392500;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51908 / MS32;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C.,
RA   Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Lykidis A., Zhao J.-S., Richardson P.;
RT   "Complete sequence of Shewanella woodyi ATCC 51908.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Alpha-galactosidase that specifically removes branched alpha-
CC       1,3-linked galactose residues present in blood group B antigens. Has no
CC       activity toward linear alpha-1,3-linked galactose residues (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-
CC         galactosidic residues, producing free D-galactose.; EC=3.2.1.n1;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC         residues in alpha-D-galactosides, including galactose
CC         oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 110 family. A subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CP000961; ACA87918.1; -; Genomic_DNA.
DR   RefSeq; WP_012326251.1; NC_010506.1.
DR   AlphaFoldDB; B1KD83; -.
DR   SMR; B1KD83; -.
DR   STRING; 392500.Swoo_3656; -.
DR   CAZy; GH110; Glycoside Hydrolase Family 110.
DR   EnsemblBacteria; ACA87918; ACA87918; Swoo_3656.
DR   KEGG; swd:Swoo_3656; -.
DR   eggNOG; COG5434; Bacteria.
DR   HOGENOM; CLU_017693_0_0_6; -.
DR   OrthoDB; 1217730at2; -.
DR   Proteomes; UP000002168; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0052692; F:raffinose alpha-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.160.20.10; -; 2.
DR   InterPro; IPR039448; Beta_helix.
DR   InterPro; IPR006626; PbH1.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   Pfam; PF13229; Beta_helix; 1.
DR   SMART; SM00710; PbH1; 5.
DR   SUPFAM; SSF51126; SSF51126; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Glycosidase; Hydrolase; Lipoprotein; Membrane; Palmitate;
KW   Reference proteome; Repeat; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           22..608
FT                   /note="Alpha-1,3-galactosidase A"
FT                   /id="PRO_5000316297"
FT   REPEAT          262..292
FT                   /note="PbH1 1"
FT   REPEAT          318..340
FT                   /note="PbH1 2"
FT   REPEAT          426..448
FT                   /note="PbH1 3"
FT   REPEAT          449..470
FT                   /note="PbH1 4"
FT   REPEAT          481..507
FT                   /note="PbH1 5"
FT   LIPID           22
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           22
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   608 AA;  68216 MW;  6691BAA70326DB2A CRC64;
     MQRRTFIKSI SAMMATSTTL GCVSNSLATE KHVSITDFGL RPNSGDDAIP ALQKALAYCK
     KHPGTSLIFP LGRYDFWSTK ADRDFYYISN NDDGIKPVAF PLTEFKNLII DGQGSRFVFH
     GGMVPVIVRE SSGITLKNFS VDWDVPFHCE GIVEAVNKKE SYVDLKIKDG FSYKVENGRF
     IFVGEGFENP VIKNLLEFDT KRQKQAYMAR DNFQREIQPV TTEIRPGVLR LQGKYPTWPK
     VGNTLLLMQE RRDWPAFSVQ DTKNTWLENI DIHHSGAMGV IAQLADGIKL DNVNIHPKDG
     SGRTVSTTVD ATHFINCKGH VVLKDCLFQG QIDDGTNVHG MYARVAEIHD SNTITFELVH
     YQQLGIDIFK PGSKVNFSDS VLNVFHDNVV DKTVRQDAKY WTVSFKEPLD TALKVDDVLD
     NMTWQPDHVM ISGCRFTGNR ARGALLTVSG KIIVENNYFS TPMMAIKIGS GGLKWYESGP
     VESVEIRNNI FDNCNYAKDS SAIDIVPGRK SKATTPYHQN VKIYQNEFRV YNERVLTTSR
     TQGISFVSNK IVKTSEYPER KSAFAPIHVE RSSNFVYQGN DFIGFGHSEL LFIDGQLVSG
     SGKLEGEG