ID   GLA_LACLA               Reviewed;         289 AA.
AC   Q9CE02;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Glycerol facilitator-aquaporin gla;
DE   AltName: Full=Aquaglyceroporin;
DE   AltName: Full=Glyceroaquaporin;
GN   Name=gla; OrderedLocusNames=LL2048; ORFNames=L117074;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- FUNCTION: Mixed channel protein that transports both water and
CC       glycerol. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA).
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
CC       {ECO:0000305}.
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DR   EMBL; AE005176; AAK06146.1; -; Genomic_DNA.
DR   PIR; H86880; H86880.
DR   RefSeq; NP_268205.1; NC_002662.1.
DR   RefSeq; WP_010906286.1; NC_002662.1.
DR   AlphaFoldDB; Q9CE02; -.
DR   SMR; Q9CE02; -.
DR   STRING; 272623.L117074; -.
DR   PaxDb; Q9CE02; -.
DR   EnsemblBacteria; AAK06146; AAK06146; L117074.
DR   KEGG; lla:L117074; -.
DR   PATRIC; fig|272623.7.peg.2205; -.
DR   eggNOG; COG0580; Bacteria.
DR   HOGENOM; CLU_020019_9_2_9; -.
DR   OMA; ACFPGRK; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015267; F:channel activity; IEA:InterPro.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd00333; MIP; 1.
DR   Gene3D; 1.20.1080.10; -; 1.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR000425; MIP.
DR   InterPro; IPR022357; MIP_CS.
DR   Pfam; PF00230; MIP; 2.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF81338; SSF81338; 1.
DR   PROSITE; PS00221; MIP; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Glycerol metabolism; Membrane; Reference proteome; Repeat;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..289
FT                   /note="Glycerol facilitator-aquaporin gla"
FT                   /id="PRO_0000064093"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        151..171
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        209..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        264..284
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           68..70
FT                   /note="NPA 1"
FT   MOTIF           235..237
FT                   /note="NPA 2"
SQ   SEQUENCE   289 AA;  30948 MW;  67472B5129B96EF2 CRC64;
     MDVTWTVKYI TEFVGTALLI IMGNGAVANV ELKGTKAHAQ SWMIIGWGYG LGVMLPAVAF
     GNITSQINPA FTLGLAASGL FPWAHVAQYI IAQVLGAMFG QLLIVMVYRP YYLKTQNPNA
     ILGTFSTIDN VDDNDKKTRL GATINGFLNE FVGSFVLFFG AVAATNIFFG SQSITWMTNY
     LKGQGADVSS SDIMNQIWVQ ASGASASKMV AHLFLGFLVM GLVVALGGPT GPGLNPARDF
     GPRLVHSLLP KSVLGEAKGS SKWWYAWVPV LAPILASLAA VALFKMIYL