ID   GLA_LACLC               Reviewed;         289 AA.
AC   P22094;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=Glycerol facilitator-aquaporin gla;
DE   AltName: Full=Aquaglyceroporin;
DE   AltName: Full=Glyceroaquaporin;
GN   Name=gla;
OS   Lactococcus lactis subsp. cremoris (Streptococcus cremoris).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=P8-2-47;
RX   PubMed=1674655; DOI=10.1128/aem.57.1.38-44.1991;
RA   Mayo B., Kok J., Venema K., Bockelmann W., Teuber M., Reinke H., Venema G.;
RT   "Molecular cloning and sequence analysis of the X-prolyl dipeptidyl
RT   aminopeptidase gene from Lactococcus lactis subsp. cremoris.";
RL   Appl. Environ. Microbiol. 57:38-44(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NCDO 763 / ML3;
RX   PubMed=1674656; DOI=10.1128/aem.57.1.45-50.1991;
RA   Nardi M., Chopin M.-C., Chopin A., Cals M.M., Gripon J.-C.;
RT   "Cloning and DNA sequence analysis of an X-prolyl dipeptidyl aminopeptidase
RT   gene from Lactococcus lactis subsp. lactis NCDO 763.";
RL   Appl. Environ. Microbiol. 57:45-50(1991).
RN   [3]
RP   CHARACTERIZATION.
RC   STRAIN=NCDO 763 / ML3;
RX   PubMed=11320116; DOI=10.1099/00221287-147-5-1129;
RA   Froger A., Rolland J.-P., Bron P., Lagree V., Le Caherec F., Deschamps S.,
RA   Hubert J.-F., Pellerin I., Thomas D., Delamarche C.;
RT   "Functional characterization of a microbial aquaglyceroporin.";
RL   Microbiology 147:1129-1135(2001).
CC   -!- FUNCTION: Mixed channel protein that transports both water and
CC       glycerol.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA).
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
CC       {ECO:0000305}.
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DR   EMBL; M58315; AAA25231.1; -; Genomic_DNA.
DR   EMBL; M35865; AAA25206.1; -; Genomic_DNA.
DR   PIR; B43747; B43747.
DR   PIR; B43748; B43748.
DR   RefSeq; WP_011677122.1; NZ_WJUX01000052.1.
DR   AlphaFoldDB; P22094; -.
DR   SMR; P22094; -.
DR   TCDB; 1.A.8.2.2; the major intrinsic protein (mip) family.
DR   GeneID; 61110371; -.
DR   OMA; ACFPGRK; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015267; F:channel activity; IEA:InterPro.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd00333; MIP; 1.
DR   Gene3D; 1.20.1080.10; -; 1.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR000425; MIP.
DR   InterPro; IPR022357; MIP_CS.
DR   Pfam; PF00230; MIP; 2.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF81338; SSF81338; 1.
DR   PROSITE; PS00221; MIP; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Glycerol metabolism; Membrane; Repeat; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..289
FT                   /note="Glycerol facilitator-aquaporin gla"
FT                   /id="PRO_0000064094"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        151..171
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        209..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        264..284
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           68..70
FT                   /note="NPA 1"
FT   MOTIF           235..237
FT                   /note="NPA 2"
SQ   SEQUENCE   289 AA;  30935 MW;  8850C2040365A268 CRC64;
     MDVTWTVKYI TEFVGTALLI IMGNGAVANV ELKGTKAHAQ SWMIIGWGYG LGVMLPAVAF
     GNITSQINPA FTLGLAASGL FPWAHVAQYI IAQVLGAMFG QLLIVMVYRP YYLKTQNPNA
     ILGTFSTIDN VDDNSEKTRL GATINGFLNE FLGSFVLFFG AVAATNIFFG SQSITWMTNY
     LKGQGADVSS SDVMNQIWVQ ASGASASKMI AHLFLGFLVM GLVVALGGPT GPGLNPARDF
     GPRLVHSLLP KSVLGEAKGS SKWWYAWVPV LAPILASLAA VALFKMIYL