GLCC_ECOLI
ID GLCC_ECOLI Reviewed; 254 AA.
AC P0ACL5; P52072; Q2M9L5;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Glc operon transcriptional activator {ECO:0000305|PubMed:8606183};
DE AltName: Full=Glc regulatory protein {ECO:0000303|PubMed:8606183};
DE AltName: Full=HTH-type transcriptional regulator GlcC;
GN Name=glcC {ECO:0000303|PubMed:8606183}; Synonyms=yghN;
GN OrderedLocusNames=b2980, JW2947;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP INDUCTION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8606183; DOI=10.1128/jb.178.7.2051-2059.1996;
RA Pellicer M.T., Badia J., Aguilar J.T., Baldoma L.;
RT "glc locus of Escherichia coli: characterization of genes encoding the
RT subunits of glycolate oxidase and the glc regulator protein.";
RL J. Bacteriol. 178:2051-2059(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, AND INDUCTION.
RC STRAIN=K12 / MC4100;
RX PubMed=9880556; DOI=10.1074/jbc.274.3.1745;
RA Pellicer M.T., Fernandez C., Badia J., Aguilar J., Lin E.C., Baldom L.;
RT "Cross-induction of glc and ace operons of Escherichia coli attributable to
RT pathway intersection. Characterization of the glc promoter.";
RL J. Biol. Chem. 274:1745-1752(1999).
CC -!- FUNCTION: Transcriptional activator of the glcDEFGB operon which is
CC associated with glycolate utilization, and encodes malate synthase G
CC and the genes needed for glycolate oxidase activity (PubMed:8606183,
CC PubMed:9880556). Also negatively regulates the transcription of its own
CC gene (PubMed:9880556). Glycolate acts as an effector, but GlcC can also
CC use acetate as an alternative effector (PubMed:9880556).
CC {ECO:0000269|PubMed:8606183, ECO:0000269|PubMed:9880556}.
CC -!- INTERACTION:
CC P0ACL5; P23893: hemL; NbExp=4; IntAct=EBI-1115389, EBI-909193;
CC -!- INDUCTION: Is constitutively expressed (PubMed:8606183). Is repressed
CC by the presence of glycolate (PubMed:9880556). GlcC exerts an
CC autogenous repression on its own gene (PubMed:9880556).
CC {ECO:0000269|PubMed:8606183, ECO:0000269|PubMed:9880556}.
CC -!- DISRUPTION PHENOTYPE: Abolishes both glycolate oxidase and malate
CC synthase G activities. Is unable to grow on glycolate as the sole
CC source of carbon, in contrast to wild type.
CC {ECO:0000269|PubMed:8606183}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA69147.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; L43490; AAB02529.1; -; Genomic_DNA.
DR EMBL; U28377; AAA69147.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76016.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77041.1; -; Genomic_DNA.
DR PIR; B65084; B65084.
DR RefSeq; NP_417454.1; NC_000913.3.
DR RefSeq; WP_001297764.1; NZ_STEB01000001.1.
DR AlphaFoldDB; P0ACL5; -.
DR SMR; P0ACL5; -.
DR BioGRID; 4262369; 17.
DR BioGRID; 851784; 56.
DR IntAct; P0ACL5; 65.
DR STRING; 511145.b2980; -.
DR PaxDb; P0ACL5; -.
DR PRIDE; P0ACL5; -.
DR EnsemblBacteria; AAC76016; AAC76016; b2980.
DR EnsemblBacteria; BAE77041; BAE77041; BAE77041.
DR GeneID; 66673130; -.
DR GeneID; 947466; -.
DR KEGG; ecj:JW2947; -.
DR KEGG; eco:b2980; -.
DR PATRIC; fig|1411691.4.peg.3750; -.
DR EchoBASE; EB2821; -.
DR eggNOG; COG2186; Bacteria.
DR HOGENOM; CLU_017584_9_1_6; -.
DR InParanoid; P0ACL5; -.
DR OMA; EHINGIR; -.
DR PhylomeDB; P0ACL5; -.
DR BioCyc; EcoCyc:G7546-MON; -.
DR PRO; PR:P0ACL5; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:EcoCyc.
DR CDD; cd07377; WHTH_GntR; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.20.120.530; -; 1.
DR InterPro; IPR011711; GntR_C.
DR InterPro; IPR008920; TF_FadR/GntR_C.
DR InterPro; IPR000524; Tscrpt_reg_HTH_GntR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF07729; FCD; 1.
DR Pfam; PF00392; GntR; 1.
DR PRINTS; PR00035; HTHGNTR.
DR SMART; SM00895; FCD; 1.
DR SMART; SM00345; HTH_GNTR; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF48008; SSF48008; 1.
DR PROSITE; PS50949; HTH_GNTR; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..254
FT /note="Glc operon transcriptional activator"
FT /id="PRO_0000050645"
FT DOMAIN 6..74
FT /note="HTH gntR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
FT DNA_BIND 34..53
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
SQ SEQUENCE 254 AA; 28826 MW; 984C172F18886BF5 CRC64;
MKDERRPICE VVAESIERLI IDGVLKVGQP LPSERRLCEK LGFSRSALRE GLTVLRGRGI
IETAQGRDSR VARLNRVQDT SPLIHLFSTQ PRTLYDLLDV RALLEGESAR LAATLGTQAD
FVVITRCYEK MLAASENNKE ISLIEHAQLD HAFHLAICQA SHNQVLVFTL QSLTDLMFNS
VFASVNNLYH RPQQKKQIDR QHARIYNAVL QRLPHVAQRA ARDHVRTVKK NLHDIELEGH
HLIRSAVPLE MNLS
