GLCI1_MOUSE
ID GLCI1_MOUSE Reviewed; 537 AA.
AC Q8K3I9; Q80YT1; Q8CEA5; Q925C1;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Glucocorticoid-induced transcript 1 protein;
DE AltName: Full=Glucocorticoid-induced gene 18 protein;
DE AltName: Full=Testhymin;
DE AltName: Full=Thymocyte/spermatocyte selection protein 1;
GN Name=Glcci1; Synonyms=Gig18, Tssn1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Leptich T.D., Flomerfelt F.A., Chapman M.S., Miesfeld R.L.;
RT "Characterization of a glucocorticoid-regulated gene in immature
RT thymocytes.";
RL Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=12557054; DOI=10.1007/s00251-002-0513-1;
RA Miazek A., Malissen B.;
RT "Two genes, three messengers: hybrid transcript between a gene expressed at
RT specific stages of T-cell and sperm maturation and an unrelated adjacent
RT gene.";
RL Immunogenetics 54:681-692(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-423 (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Skin;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic brain;
RX PubMed=15345747; DOI=10.1074/mcp.m400085-mcp200;
RA Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.;
RT "Phosphoproteomic analysis of the developing mouse brain.";
RL Mol. Cell. Proteomics 3:1093-1101(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96; SER-98; SER-99; THR-101;
RP SER-293; SER-335; THR-340; SER-384 AND SER-402, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8K3I9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8K3I9-2; Sequence=VSP_021322, VSP_021323;
CC Name=3;
CC IsoId=Q8K3I9-3; Sequence=VSP_021321;
CC -!- TISSUE SPECIFICITY: Predominantly expressed in thymus and testis,
CC especially in CD4+CD8+ cells and at specific stages of spermatogenesis.
CC {ECO:0000269|PubMed:12557054}.
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DR EMBL; AF374476; AAK54615.1; -; mRNA.
DR EMBL; AY098636; AAM33072.1; -; mRNA.
DR EMBL; BC050802; AAH50802.1; -; mRNA.
DR EMBL; AK028709; BAC26078.1; -; mRNA.
DR CCDS; CCDS71725.1; -. [Q8K3I9-3]
DR RefSeq; NP_001273657.1; NM_001286728.1.
DR RefSeq; NP_001273658.1; NM_001286729.1. [Q8K3I9-3]
DR RefSeq; NP_573499.2; NM_133236.3.
DR AlphaFoldDB; Q8K3I9; -.
DR STRING; 10090.ENSMUSP00000069444; -.
DR iPTMnet; Q8K3I9; -.
DR PhosphoSitePlus; Q8K3I9; -.
DR jPOST; Q8K3I9; -.
DR MaxQB; Q8K3I9; -.
DR PaxDb; Q8K3I9; -.
DR PeptideAtlas; Q8K3I9; -.
DR PRIDE; Q8K3I9; -.
DR ProteomicsDB; 267630; -. [Q8K3I9-1]
DR ProteomicsDB; 267631; -. [Q8K3I9-2]
DR ProteomicsDB; 267632; -. [Q8K3I9-3]
DR Antibodypedia; 945; 183 antibodies from 20 providers.
DR DNASU; 170772; -.
DR Ensembl; ENSMUST00000161217; ENSMUSP00000124167; ENSMUSG00000029638. [Q8K3I9-3]
DR GeneID; 170772; -.
DR KEGG; mmu:170772; -.
DR UCSC; uc009axn.3; mouse. [Q8K3I9-2]
DR UCSC; uc009axu.2; mouse. [Q8K3I9-1]
DR UCSC; uc009axw.1; mouse. [Q8K3I9-3]
DR CTD; 113263; -.
DR MGI; MGI:2179717; Glcci1.
DR VEuPathDB; HostDB:ENSMUSG00000029638; -.
DR eggNOG; ENOG502QRQF; Eukaryota.
DR GeneTree; ENSGT00950000183046; -.
DR InParanoid; Q8K3I9; -.
DR OrthoDB; 1132072at2759; -.
DR PhylomeDB; Q8K3I9; -.
DR BioGRID-ORCS; 170772; 0 hits in 72 CRISPR screens.
DR ChiTaRS; Glcci1; mouse.
DR PRO; PR:Q8K3I9; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q8K3I9; protein.
DR Bgee; ENSMUSG00000029638; Expressed in animal zygote and 85 other tissues.
DR ExpressionAtlas; Q8K3I9; baseline and differential.
DR Genevisible; Q8K3I9; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR InterPro; IPR026639; Glcci1.
DR InterPro; IPR026642; Glcci1/FAM117.
DR PANTHER; PTHR14972; PTHR14972; 1.
DR PANTHER; PTHR14972:SF3; PTHR14972:SF3; 1.
DR Pfam; PF15388; FAM117; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Phosphoprotein; Reference proteome.
FT CHAIN 1..537
FT /note="Glucocorticoid-induced transcript 1 protein"
FT /id="PRO_0000256129"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 62..254
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 309..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 495..520
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 217..244
FT /evidence="ECO:0000255"
FT COMPBIAS 1..21
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 95..109
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 114..133
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 154..170
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 236..250
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 328..372
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 388..402
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 69
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86VQ1"
FT MOD_RES 96
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 98
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 99
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 101
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 162
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86VQ1"
FT MOD_RES 163
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86VQ1"
FT MOD_RES 166
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q86VQ1"
FT MOD_RES 168
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q86VQ1"
FT MOD_RES 214
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86VQ1"
FT MOD_RES 248
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86VQ1"
FT MOD_RES 256
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q86VQ1"
FT MOD_RES 293
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 333
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q86VQ1"
FT MOD_RES 335
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 340
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 384
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 388
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86VQ1"
FT MOD_RES 396
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86VQ1"
FT MOD_RES 402
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 470
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86VQ1"
FT VAR_SEQ 1..187
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_021321"
FT VAR_SEQ 1..107
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_021322"
FT VAR_SEQ 108..143
FT /note="PAEQAPRAKGRPRRSPESRRRSSSPERRSPGSPVCR -> MCPSPWPHMCWR
FT HRAPSATFLTTYSHTMSATTCPDS (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_021323"
FT CONFLICT 223
FT /note="E -> EQ (in Ref. 3; AAH50802 and 4; BAC26078)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 537 AA; 57479 MW; A0B1D63CAC7534EE CRC64;
MSTASSSSSQ TPHSAPQRMR RSTAGSPPAA AGSGTGPAGS CAPAAGAGRL LQPIRATVPY
QLLRGSQHSP TRPAAAATAA AALGSLSGPG GARGPSPSSP TPPPAAAPAE QAPRAKGRPR
RSPESRRRSS SPERRSPGSP VCRVDRPKSQ HIRTSSTIRR TSSLDTITGP YLTGQWPRDP
HVHYPSCMRD KATQTPSCWA EEGAEKRSHQ RSASWGSADQ LKEIAKLRQQ LQRSKQSSRH
SKEKDRQSPL HGNHITISHT QAIGSRSVPM PLSNISVPKS SVSRVPCNVE GISPELEKVF
IKENNGKEEV SKPLDIPDGR RAPLPAHYRS SSTRSIDTQT PSVQERSSSC SSHSPCVSPF
CPPESQDGSP CSTEDLLYDR DKDSGSSSPL PKYASSPKPN NSYMFKREPP EGCERVKVFE
EMASRQPISA PLFSCPDKNK VNFIPTGSAF CPVKLLGPLL PASDLMLKNS PNSGQSSALA
TLTVEQLSSR VSFTSLSDDT STADSLEPSA QQPSQQQQLL QDLQVEEHVS TQNYVMI
