GLCM2_CAEEL
ID GLCM2_CAEEL Reviewed; 516 AA.
AC O16581;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Putative glucosylceramidase 2;
DE EC=3.2.1.45;
DE Flags: Precursor;
GN Name=gba-2; ORFNames=C33C12.8;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC -!- PATHWAY: Lipid metabolism; sphingolipid metabolism.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 30 family. {ECO:0000305}.
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DR EMBL; FO080754; CCD66456.1; -; Genomic_DNA.
DR RefSeq; NP_494208.2; NM_061807.5.
DR AlphaFoldDB; O16581; -.
DR SMR; O16581; -.
DR BioGRID; 47987; 3.
DR DIP; DIP-25825N; -.
DR STRING; 6239.C33C12.8; -.
DR CAZy; GH30; Glycoside Hydrolase Family 30.
DR PaxDb; O16581; -.
DR PeptideAtlas; O16581; -.
DR EnsemblMetazoa; C33C12.8.1; C33C12.8.1; WBGene00016340.
DR GeneID; 183155; -.
DR KEGG; cel:CELE_C33C12.8; -.
DR UCSC; C33C12.8; c. elegans.
DR CTD; 183155; -.
DR WormBase; C33C12.8; CE41877; WBGene00016340; gba-2.
DR eggNOG; KOG2566; Eukaryota.
DR GeneTree; ENSGT00390000009464; -.
DR HOGENOM; CLU_014379_1_2_1; -.
DR InParanoid; O16581; -.
DR OMA; VWMTTEN; -.
DR OrthoDB; 644299at2759; -.
DR PhylomeDB; O16581; -.
DR UniPathway; UPA00222; -.
DR PRO; PR:O16581; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00016340; Expressed in adult organism and 1 other tissue.
DR GO; GO:0004348; F:glucosylceramidase activity; IBA:GO_Central.
DR GO; GO:0006680; P:glucosylceramide catabolic process; IBA:GO_Central.
DR InterPro; IPR033452; GH30_C.
DR InterPro; IPR001139; Glyco_hydro_30.
DR InterPro; IPR033453; Glyco_hydro_30_TIM-barrel.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR11069; PTHR11069; 1.
DR Pfam; PF02055; Glyco_hydro_30; 1.
DR Pfam; PF17189; Glyco_hydro_30C; 1.
DR PRINTS; PR00843; GLHYDRLASE30.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 3: Inferred from homology;
KW Hydrolase; Lipid metabolism; Reference proteome; Signal;
KW Sphingolipid metabolism.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..516
FT /note="Putative glucosylceramidase 2"
FT /id="PRO_0000421454"
FT ACT_SITE 254
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 358
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 516 AA; 57783 MW; D7A02EB80C218ED6 CRC64;
MSIAWSCFVL GLFALASLQV ALANDCAQKT FKTGIVCVCN ITYCDEIPDI NLLSGQAATF
TTSKSGARLH RDVVYATNSD PLTSMHFTID SSKTYQTIQG FGSTFSDASG ANLKSLPDQM
ADTILRQYFS DSGLNLQFGR VPIASNDFSS RVYTYDDNLE DYNMAHFSLQ REDYQWKIPY
MQMAQKYNHD LKFFAVPWSA PGWLKTTNST KGYGILLGTN QDTYHKSYVT YILHFLEEYQ
KNGILFWGLS TQNEPTSGSD KKTKMQSMGF TAEFQRDFIK LDIGPALKSS NAGKNVKILI
LDDNRGNLPK WADTVLNDKD AASYVSGIAV HSYQDDESDK HLTQTHNNHP DVFIFGTEAS
EGSKSKDVDY GSFDRAEDYV SDILDDFNNW VTGWTERNLV LDAQGGPSWV SGFADAPVIA
FPALAQFYKQ PMFYAIAHFS HFLKPGAVRI DHSLNMPNPE IERSAFLNPD GSKVVVLHNK
NPLAPYSLSI KDTMKSTDHY QVHLSPKTIV TLYIQN
