GLCM3_CAEEL
ID GLCM3_CAEEL Reviewed; 522 AA.
AC G5ECR8; G5EEA6; G5EFK0; Q8MQ76;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Putative glucosylceramidase 3;
DE EC=3.2.1.45;
DE Flags: Precursor;
GN Name=gba-3 {ECO:0000312|WormBase:F11E6.1a};
GN ORFNames=F11E6.1 {ECO:0000312|WormBase:F11E6.1a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC -!- PATHWAY: Lipid metabolism; sphingolipid metabolism.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=a {ECO:0000312|WormBase:F11E6.1a};
CC IsoId=G5ECR8-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:F11E6.1b};
CC IsoId=G5ECR8-2; Sequence=VSP_045726, VSP_045727, VSP_045728,
CC VSP_045729, VSP_045730, VSP_045731,
CC VSP_045732, VSP_045733, VSP_045734,
CC VSP_045735, VSP_045736;
CC Name=d {ECO:0000312|WormBase:F11E6.1d};
CC IsoId=G5ECR8-4; Sequence=VSP_045725;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 30 family. {ECO:0000305}.
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DR EMBL; BX284604; CCD31056.1; -; Genomic_DNA.
DR EMBL; AL031254; CCD31056.1; JOINED; Genomic_DNA.
DR EMBL; BX284604; CAB02924.1; -; Genomic_DNA.
DR EMBL; AL031254; CAB02924.1; JOINED; Genomic_DNA.
DR EMBL; BX284604; CAC35813.1; -; Genomic_DNA.
DR EMBL; AL031254; CAC35813.1; JOINED; Genomic_DNA.
DR PIR; T18583; T18583.
DR RefSeq; NP_001255965.1; NM_001269036.1. [G5ECR8-4]
DR RefSeq; NP_503118.1; NM_070717.3. [G5ECR8-2]
DR RefSeq; NP_503119.1; NM_070718.3. [G5ECR8-1]
DR AlphaFoldDB; G5ECR8; -.
DR SMR; G5ECR8; -.
DR BioGRID; 43610; 7.
DR IntAct; G5ECR8; 1.
DR MINT; G5ECR8; -.
DR STRING; 6239.F11E6.1d; -.
DR CAZy; GH30; Glycoside Hydrolase Family 30.
DR EPD; G5ECR8; -.
DR PaxDb; G5ECR8; -.
DR PeptideAtlas; G5ECR8; -.
DR EnsemblMetazoa; F11E6.1a.1; F11E6.1a.1; WBGene00008706. [G5ECR8-1]
DR EnsemblMetazoa; F11E6.1b.1; F11E6.1b.1; WBGene00008706. [G5ECR8-2]
DR EnsemblMetazoa; F11E6.1d.1; F11E6.1d.1; WBGene00008706. [G5ECR8-4]
DR GeneID; 178535; -.
DR KEGG; cel:CELE_F11E6.1; -.
DR UCSC; F11E6.1a; c. elegans.
DR CTD; 178535; -.
DR WormBase; F11E6.1a; CE20669; WBGene00008706; gba-3. [G5ECR8-1]
DR WormBase; F11E6.1b; CE26698; WBGene00008706; gba-3. [G5ECR8-2]
DR WormBase; F11E6.1d; CE46394; WBGene00008706; gba-3. [G5ECR8-4]
DR eggNOG; KOG2566; Eukaryota.
DR GeneTree; ENSGT00390000009464; -.
DR InParanoid; G5ECR8; -.
DR OMA; PIMGDWF; -.
DR OrthoDB; 644299at2759; -.
DR PhylomeDB; G5ECR8; -.
DR Reactome; R-CEL-1660662; Glycosphingolipid metabolism.
DR UniPathway; UPA00222; -.
DR PRO; PR:G5ECR8; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00008706; Expressed in larva and 4 other tissues.
DR GO; GO:0004348; F:glucosylceramidase activity; IBA:GO_Central.
DR GO; GO:0006680; P:glucosylceramide catabolic process; IBA:GO_Central.
DR InterPro; IPR033452; GH30_C.
DR InterPro; IPR001139; Glyco_hydro_30.
DR InterPro; IPR033453; Glyco_hydro_30_TIM-barrel.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR11069; PTHR11069; 2.
DR Pfam; PF02055; Glyco_hydro_30; 1.
DR Pfam; PF17189; Glyco_hydro_30C; 1.
DR PRINTS; PR00843; GLHYDRLASE30.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Hydrolase; Lipid metabolism; Reference proteome;
KW Signal; Sphingolipid metabolism.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..522
FT /note="Putative glucosylceramidase 3"
FT /id="PRO_0000421455"
FT ACT_SITE 259
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 364
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..18
FT /note="MSRWKVVILCLLSFMFEI -> MAFFSVRSSKIVFGLERSEEK (in
FT isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_045725"
FT VAR_SEQ 370..371
FT /note="FG -> AI (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_045726"
FT VAR_SEQ 376
FT /note="I -> L (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_045727"
FT VAR_SEQ 382
FT /note="R -> T (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_045728"
FT VAR_SEQ 385
FT /note="S -> N (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_045729"
FT VAR_SEQ 388
FT /note="D -> S (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_045730"
FT VAR_SEQ 392
FT /note="T -> S (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_045731"
FT VAR_SEQ 398
FT /note="V -> F (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_045732"
FT VAR_SEQ 410..411
FT /note="ET -> DM (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_045733"
FT VAR_SEQ 415..420
FT /note="NWAYNV -> TWVDNF (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_045734"
FT VAR_SEQ 431
FT /note="A -> G (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_045735"
FT VAR_SEQ 443
FT /note="L -> M (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_045736"
SQ SEQUENCE 522 AA; 59088 MW; 3CD73EE50CFBA7C7 CRC64;
MSRWKVVILC LLSFMFEIGH ASFQCNPKTY DGAFLNIVCV CNATFCDEIE PIGEIAEGKA
IVYRSSLDGD RLKRMSMKMK EKLRKNESVN VTITIDASER FQNIFGFGGA FTDSAGDQFV
SLSETLQNYI VDSYFGKNGL EYNIGRVPIA SCDFSTHEYS YDDVHDDFEL KHFALPDEDL
KLKIPFIKKA IEKTEGNIQL FASPWSAPGW MKVTGRMRGG GAMRNDKRVY QAYADYFFKF
FEAYSSHAIT FWGLTIQNEP STGADMAWRW QTMNYTAETM RDFLKKYLGP KLKENKLTET
LKVMVLDDGR GLLPGWADTI FNDPEATKYA DGVAVHWYGN LYSPAVLLDI TQRHHPTKFI
FGTEACAGYF GHHGPIMGDW FRAESYADDI ITDLNHHVTG WTDWNLCLDE TGGPNWAYNV
VDSPIIVNRT AQEFYKQPMF YALGHFSKFL PRGSTRVFTK IEGNLAVSAT SVVIEGGRRA
TVILSKASNS LLTRIVDSST GFSIVLNLPP RSIHTVIWKK RK
