GLCM4_CAEEL
ID GLCM4_CAEEL Reviewed; 519 AA.
AC Q9UB00;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Putative glucosylceramidase 4;
DE EC=3.2.1.45;
DE Flags: Precursor;
GN Name=gba-4; ORFNames=Y4C6B.6;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC -!- PATHWAY: Lipid metabolism; sphingolipid metabolism.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 30 family. {ECO:0000305}.
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DR EMBL; FO081369; CCD71128.1; -; Genomic_DNA.
DR PIR; T34017; T34017.
DR RefSeq; NP_500785.1; NM_068384.4.
DR AlphaFoldDB; Q9UB00; -.
DR SMR; Q9UB00; -.
DR BioGRID; 42437; 9.
DR STRING; 6239.Y4C6B.6; -.
DR CAZy; GH30; Glycoside Hydrolase Family 30.
DR EPD; Q9UB00; -.
DR PaxDb; Q9UB00; -.
DR PeptideAtlas; Q9UB00; -.
DR EnsemblMetazoa; Y4C6B.6.1; Y4C6B.6.1; WBGene00021160.
DR GeneID; 177314; -.
DR KEGG; cel:CELE_Y4C6B.6; -.
DR UCSC; Y4C6B.6; c. elegans.
DR CTD; 177314; -.
DR WormBase; Y4C6B.6; CE28522; WBGene00021160; gba-4.
DR eggNOG; KOG2566; Eukaryota.
DR GeneTree; ENSGT00390000009464; -.
DR HOGENOM; CLU_014379_1_2_1; -.
DR InParanoid; Q9UB00; -.
DR OMA; IMINDDQ; -.
DR OrthoDB; 644299at2759; -.
DR PhylomeDB; Q9UB00; -.
DR UniPathway; UPA00222; -.
DR PRO; PR:Q9UB00; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00021160; Expressed in adult organism and 2 other tissues.
DR GO; GO:0004348; F:glucosylceramidase activity; IBA:GO_Central.
DR GO; GO:0006680; P:glucosylceramide catabolic process; IBA:GO_Central.
DR InterPro; IPR033452; GH30_C.
DR InterPro; IPR001139; Glyco_hydro_30.
DR InterPro; IPR033453; Glyco_hydro_30_TIM-barrel.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR11069; PTHR11069; 1.
DR Pfam; PF02055; Glyco_hydro_30; 1.
DR Pfam; PF17189; Glyco_hydro_30C; 1.
DR PRINTS; PR00843; GLHYDRLASE30.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 3: Inferred from homology;
KW Hydrolase; Lipid metabolism; Reference proteome; Signal;
KW Sphingolipid metabolism.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..519
FT /note="Putative glucosylceramidase 4"
FT /id="PRO_0000421456"
FT ACT_SITE 256
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 361
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 519 AA; 58247 MW; FD822081D820C80A CRC64;
MILNISVSLL IFLAFYGFSS DAKSLPCSEV KKEYGIVCRC NATYCDTIEP LGTVTSGKAV
VYTTSRNGKR MNRSELKHTT SSTAKTKVYV NTTQSFQPVM GFGAAFTDAA GINMKMLPQT
MQDQIIQQYF SDDGLGYVFG RVPMASTDFS THEYSYDDVK FDFDLKNFNL TVEDLQYKIP
FIKKAMTASG GKLKLFATPW SSPGWMKTSG RMVGAGELLG DQNGKYYQTW AQYFVKFFEA
YHAQGIDFWS LTPQNEPTTG IDPLWKWQTL FFDASMERNF IKKLLGPALA SSPVTKNLKI
MINDDQRINL PHWPNVILTD PTAAQYVHGI AIHWYEDFID PATVLTETHE KFPDYFLLAT
EACAGYFPAD GPKLGSWSRA EQYANDLIKD MGNWVGGWVD WNYILDLQGG PNLAKNFVDS
TIIVNATAQE YYKQPIWHVM AQFSKFVKPG AIRVGINIIE KSVDVEGLSF LNQDGTKTVV
LLNKNEVLSF DVAISDVSAP NVIYDLTIQP NSLITIVYK
