ID   GLCT_STACT              Reviewed;         287 AA.
AC   O33618; B9DP61;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=GlcA/glcB genes antiterminator;
DE   AltName: Full=PtsG operon antiterminator;
GN   Name=glcT; OrderedLocusNames=Sca_0998;
OS   Staphylococcus carnosus (strain TM300).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=396513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, FUNCTION AS AN
RP   ANTITERMINATOR, MASS SPECTROMETRY, SUBUNIT, AND PHOSPHORYLATION AT HIS-105.
RX   PubMed=10974121; DOI=10.1099/00221287-146-9-2333;
RA   Knezevic I., Bachem S., Sickmann A., Meyer H.E., Stuelke J.,
RA   Hengstenberg W.;
RT   "Regulation of the glucose-specific phosphotransferase system (PTS) of
RT   Staphylococcus carnosus by the antiterminator protein GlcT.";
RL   Microbiology 146:2333-2342(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TM300;
RX   PubMed=19060169; DOI=10.1128/aem.01982-08;
RA   Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C.,
RA   Goetz F.;
RT   "Genome analysis of the meat starter culture bacterium Staphylococcus
RT   carnosus TM300.";
RL   Appl. Environ. Microbiol. 75:811-822(2009).
CC   -!- FUNCTION: Mediates positive regulation of the ptsG/glcA and glcB genes
CC       by functioning as an antiterminator factor of transcription via its
CC       interaction with the RNA-antiterminator (RAT) sequence located upstream
CC       of the ptsG/glcA gene. {ECO:0000269|PubMed:10974121}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:10974121}.
CC   -!- PTM: Phosphorylated by HPr, which enhances dimer formation and leads to
CC       activation. {ECO:0000269|PubMed:10974121}.
CC   -!- MASS SPECTROMETRY: Mass=33504; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:10974121};
CC   -!- MASS SPECTROMETRY: Mass=33586; Method=Electrospray; Note=With
CC       phosphorylation at His-105.; Evidence={ECO:0000269|PubMed:10974121};
CC   -!- SIMILARITY: Belongs to the transcriptional antiterminator BglG family.
CC       GlcT subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Y14029; CAA74358.1; -; Genomic_DNA.
DR   EMBL; AM295250; CAL27906.1; -; Genomic_DNA.
DR   RefSeq; WP_015900247.1; NC_012121.1.
DR   AlphaFoldDB; O33618; -.
DR   SMR; O33618; -.
DR   STRING; 396513.SCA_0998; -.
DR   iPTMnet; O33618; -.
DR   GeneID; 60545311; -.
DR   KEGG; sca:SCA_0998; -.
DR   eggNOG; COG3711; Bacteria.
DR   HOGENOM; CLU_078802_0_0_9; -.
DR   OMA; VMQEILN; -.
DR   OrthoDB; 1193348at2; -.
DR   BioCyc; SCAR396513:SCA_RS05010-MON; -.
DR   Proteomes; UP000000444; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.30.24.10; -; 1.
DR   InterPro; IPR004341; CAT_RNA-bd_dom.
DR   InterPro; IPR036650; CAT_RNA-bd_dom_sf.
DR   InterPro; IPR011608; PRD.
DR   InterPro; IPR036634; PRD_sf.
DR   InterPro; IPR001550; Transcrpt_antitermin_CS.
DR   Pfam; PF03123; CAT_RBD; 1.
DR   Pfam; PF00874; PRD; 2.
DR   SMART; SM01061; CAT_RBD; 1.
DR   SUPFAM; SSF50151; SSF50151; 1.
DR   SUPFAM; SSF63520; SSF63520; 2.
DR   PROSITE; PS00654; PRD_1; 1.
DR   PROSITE; PS51372; PRD_2; 2.
PE   1: Evidence at protein level;
KW   Activator; Direct protein sequencing; Phosphoprotein; Repeat; RNA-binding;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..287
FT                   /note="GlcA/glcB genes antiterminator"
FT                   /id="PRO_0000351420"
FT   DOMAIN          67..175
FT                   /note="PRD 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
FT   DOMAIN          176..284
FT                   /note="PRD 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
FT   MOD_RES         105
FT                   /note="Phosphohistidine; by HPr"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00704,
FT                   ECO:0000269|PubMed:10974121"
SQ   SEQUENCE   287 AA;  33632 MW;  74D719BC1F538B34 CRC64;
     MSNYVIEKTL NNNVIICTDE NQHHEVVLIG KGIGFNKKKG MELSDSVMID KVYKLEQKKD
     QDHYKALVEI ADDNVLQTII EAMDIITHAD RTVVDKDLMV ALTDHILFAY KRIKQHQFIK
     NPFLIETKQL YSESYQIAVS VIEHLNKLLD IEFPEDEIGF IALHIASSKD DLSLHEVRLT
     NEIINKSILI MEHDLKYKID TNSIQYQRFI RHIQFLIRRL QKGEIIQVND EFGNMLKAHY
     PLCYNIAVKI IKMMQQHLDV EVYEAELIYL TLHINHFTQQ NEKNTNV