GLD1_YEAST
ID GLD1_YEAST Reviewed; 294 AA.
AC Q06104; D6W4A8; Q6B2S3;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Golgi/endosome-localized DSC protein 1 {ECO:0000303|PubMed:29355480};
GN Name=GLD1 {ECO:0000303|PubMed:29355480}; OrderedLocusNames=YPR109W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP INDUCTION.
RX PubMed=12370439; DOI=10.1073/pnas.202495299;
RA Williams R.M., Primig M., Washburn B.K., Winzeler E.A., Bellis M.,
RA Sarrauste de Menthiere C., Davis R.W., Easton Esposito R.;
RT "The Ume6 regulon coordinates metabolic and meiotic gene expression in
RT yeast.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:13431-13436(2002).
RN [5]
RP FUNCTION.
RX PubMed=12829295; DOI=10.1016/s0378-1097(03)00384-7;
RA Zettel M.F., Garza L.R., Cass A.M., Myhre R.A., Haizlip L.A., Osadebe S.N.,
RA Sudimack D.W., Pathak R., Stone T.L., Polymenis M.;
RT "The budding index of Saccharomyces cerevisiae deletion strains identifies
RT genes important for cell cycle progression.";
RL FEMS Microbiol. Lett. 223:253-258(2003).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND IDENTIFICATION IN THE
RP GOLGI/ENDOSOME-LOCALIZED DSC COMPLEX.
RX PubMed=29355480; DOI=10.7554/elife.33116;
RA Yang X., Arines F.M., Zhang W., Li M.;
RT "Sorting of a multi-subunit ubiquitin ligase complex in the endolysosome
RT system.";
RL Elife 7:0-0(2018).
CC -!- FUNCTION: Component of the Golgi/endosome-localized DSC E3 ubiquitin
CC ligase complex involved in the targeting of the DSC complex to the
CC Golgi apparatus and endosome membranes via the AP3 pathway to
CC ubiquinate Golgi/endosome membrane proteins (PubMed:29355480). Competes
CC with VLD1 to determine the subcellular localizations of the DSC complex
CC (PubMed:29355480). May be required for cell cycle progression
CC (PubMed:12829295). {ECO:0000269|PubMed:12829295,
CC ECO:0000269|PubMed:29355480}.
CC -!- SUBUNIT: Component of the Golgi/endosome-localized DSC E3 ligase
CC complex composed of at least TUL1, DSC2, DSC3, UBX3, CDC48 and GLD1.
CC {ECO:0000269|PubMed:29355480}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:29355480}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:12829295}. Endosome membrane
CC {ECO:0000269|PubMed:29355480}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:12829295}.
CC -!- INDUCTION: Expression is regulated by the metabolic and meiotic
CC transcriptional regulator UME6. {ECO:0000269|PubMed:12370439}.
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DR EMBL; U32445; AAB68079.1; -; Genomic_DNA.
DR EMBL; AY692657; AAT92676.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11524.1; -; Genomic_DNA.
DR PIR; S59774; S59774.
DR RefSeq; NP_015434.1; NM_001184206.1.
DR AlphaFoldDB; Q06104; -.
DR BioGRID; 36276; 137.
DR STRING; 4932.YPR109W; -.
DR PaxDb; Q06104; -.
DR PRIDE; Q06104; -.
DR EnsemblFungi; YPR109W_mRNA; YPR109W; YPR109W.
DR GeneID; 856225; -.
DR KEGG; sce:YPR109W; -.
DR SGD; S000006313; YPR109W.
DR VEuPathDB; FungiDB:YPR109W; -.
DR eggNOG; ENOG502S4TY; Eukaryota.
DR HOGENOM; CLU_957155_0_0_1; -.
DR InParanoid; Q06104; -.
DR OMA; NSDGYDG; -.
DR BioCyc; YEAST:G3O-34249-MON; -.
DR PRO; PR:Q06104; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q06104; protein.
DR GO; GO:0044695; C:Dsc E3 ubiquitin ligase complex; IDA:SGD.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0031303; C:integral component of endosome membrane; ISM:SGD.
DR GO; GO:0030173; C:integral component of Golgi membrane; ISM:SGD.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0031503; P:protein-containing complex localization; IMP:SGD.
DR InterPro; IPR013715; DUF1746.
DR Pfam; PF08508; DUF1746; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Endosome; Golgi apparatus; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..294
FT /note="Golgi/endosome-localized DSC protein 1"
FT /id="PRO_0000242625"
FT TOPO_DOM 1..139
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..215
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 237..294
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 11
FT /note="N -> Y (in Ref. 3; AAT92676)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 294 AA; 32710 MW; BD87F4D30B167647 CRC64;
MDNFESTAEG NLSIGNKRVY ELRKRNFQRN LVNNLSFLGY VLISLEYIKY DRTVWTLITR
AIVQSLISSP FPSDAKLRRL ATLGADNNTT GVATLPGGRS IRFPGMFGTE MLYNSSSEAE
QQDHDDTAIV SMKKQIRKFL FHGCLSLNML FIILTILFPI DFFEPLSGSE PVDDGPKNTP
SPFSNSDGLL LGERRGGLFL QMIGERLPKS NFSGNLGLVM FEFSILIVQF TLFSLTCVVL
ADLDFEEPER LEPVNSDGYD GSVIVARIPL NKTMNAILND GNINDNNENA SNSV
