GLDH_FLAJ1
ID GLDH_FLAJ1 Reviewed; 159 AA.
AC Q8KRP0; A5FLJ9;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Gliding motility lipoprotein GldH;
DE Flags: Precursor;
GN Name=gldH; OrderedLocusNames=Fjoh_0890;
OS Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC
OS 14942 / NCIMB 11054 / UW101) (Cytophaga johnsonae).
OC Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=376686;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION,
RP DIACYLGLYCEROL AT CYS-19, PALMITOYLATION AT CYS-19, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=14594839; DOI=10.1128/jb.185.22.6648-6657.2003;
RA McBride M.J., Braun T.F., Brust J.L.;
RT "Flavobacterium johnsoniae GldH is a lipoprotein that is required for
RT gliding motility and chitin utilization.";
RL J. Bacteriol. 185:6648-6657(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101;
RX PubMed=19717629; DOI=10.1128/aem.01495-09;
RA McBride M.J., Xie G., Martens E.C., Lapidus A., Henrissat B., Rhodes R.G.,
RA Goltsman E., Wang W., Xu J., Hunnicutt D.W., Staroscik A.M., Hoover T.R.,
RA Cheng Y.Q., Stein J.L.;
RT "Novel features of the polysaccharide-digesting gliding bacterium
RT Flavobacterium johnsoniae as revealed by genome sequence analysis.";
RL Appl. Environ. Microbiol. 75:6864-6875(2009).
CC -!- FUNCTION: Required for gliding motility and chitin utilization.
CC {ECO:0000269|PubMed:14594839}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00303, ECO:0000269|PubMed:14594839}; Lipid-anchor
CC {ECO:0000255|PROSITE-ProRule:PRU00303, ECO:0000269|PubMed:14594839}.
CC -!- DISRUPTION PHENOTYPE: Cells form nonspreading colonies, cannot digest
CC chitin and are resistant to bacteriophages. Whereas wild-type cells can
CC bind latex spheres and propel them along their surface, cells of the
CC gldH mutant fail to do it. {ECO:0000269|PubMed:14594839}.
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DR EMBL; AF527791; AAM92019.1; -; Genomic_DNA.
DR EMBL; CP000685; ABQ03924.1; -; Genomic_DNA.
DR RefSeq; WP_012022977.1; NZ_MUGZ01000025.1.
DR AlphaFoldDB; Q8KRP0; -.
DR STRING; 376686.Fjoh_0890; -.
DR EnsemblBacteria; ABQ03924; ABQ03924; Fjoh_0890.
DR KEGG; fjo:Fjoh_0890; -.
DR eggNOG; ENOG50313I2; Bacteria.
DR HOGENOM; CLU_109250_2_0_10; -.
DR OMA; KLWYKEN; -.
DR OrthoDB; 1850223at2; -.
DR Proteomes; UP000006694; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0071976; P:cell gliding; IMP:CACAO.
DR GO; GO:0006032; P:chitin catabolic process; IMP:CACAO.
DR InterPro; IPR020018; Motility-assoc_lipoprot_GldH.
DR Pfam; PF14109; GldH_lipo; 1.
DR TIGRFAMs; TIGR03511; GldH_lipo; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Lipoprotein; Membrane; Palmitate; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 19..159
FT /note="Gliding motility lipoprotein GldH"
FT /id="PRO_0000277812"
FT LIPID 19
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303,
FT ECO:0000269|PubMed:14594839"
FT LIPID 19
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303,
FT ECO:0000269|PubMed:14594839"
SQ SEQUENCE 159 AA; 18067 MW; 8A76310599E1F514 CRC64;
MRIKNSGILL LAAILLFSCD KKRVFDEYKS VGSAWHKDSV VTFDLPVLDS TKKYNLFVNL
RDNNNYPFNN LFLIVAIETP SGFTKVDTLE YQMANPDGTL MGNGFTDIKE SKLYYKEDVK
FKGKYKVHIK QAVRESGKIP GVEALEGITD VGFRIEQKD
