GLFT1_MYCS2
ID GLFT1_MYCS2 Reviewed; 302 AA.
AC A0R5Z2; I7FMT0;
DT 13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Galactofuranosyltransferase GlfT1 {ECO:0000305};
DE Short=GalTr 1 {ECO:0000305};
DE EC=2.4.1.287 {ECO:0000269|PubMed:18055597};
DE AltName: Full=Arabinogalactan galactosyltransferase 1 {ECO:0000305};
DE AltName: Full=Rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,4/1,5-galactofuranosyltransferase;
DE AltName: Full=UDP-Galf:alpha-3-L-rhamnosyl-alpha-D-GlcNAc-pyrophosphate polyprenol, galactofuranosyl transferase;
GN Name=glfT1 {ECO:0000303|PubMed:18055597};
GN OrderedLocusNames=MSMEG_6367, MSMEI_6199;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION, CATALYTIC ACTIVITY, PATHWAY, AND SUBSTRATE SPECIFICITY.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18055597; DOI=10.1128/jb.01326-07;
RA Belanova M., Dianiskova P., Brennan P.J., Completo G.C., Rose N.L.,
RA Lowary T.L., Mikusova K.;
RT "Galactosyl transferases in mycobacterial cell wall synthesis.";
RL J. Bacteriol. 190:1141-1145(2008).
CC -!- FUNCTION: Involved in the biosynthesis of the arabinogalactan (AG)
CC region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an
CC essential component of the mycobacterial cell wall. Catalyzes the
CC transfer of the first two galactofuranosyl (Galf) units from UDP-
CC galactofuranose (UDP-Galf) onto the rhamnosyl-GlcNAc-diphospho-
CC decaprenol (Rha-GlcNAc-PP-C50) acceptor, yielding galactofuranosyl-
CC galactofuranosyl-rhamnosyl-GlcNAc-diphospho-decaprenol (Galf-Galf-Rha-
CC GlcNAc-PP-C50). Thus, GlfT1 is the initiator of galactan synthesis,
CC while GlfT2 continues with the subsequent polymerization events.
CC {ECO:0000269|PubMed:18055597}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-L-rhamnosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-
CC diphospho-trans,octa-cis-decaprenol + 2 UDP-alpha-D-galactofuranose =
CC beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-
CC L-rhamnosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-
CC trans,octa-cis-decaprenol + 2 H(+) + 2 UDP; Xref=Rhea:RHEA:34379,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:66915,
CC ChEBI:CHEBI:67209, ChEBI:CHEBI:67210; EC=2.4.1.287;
CC Evidence={ECO:0000269|PubMed:18055597};
CC -!- PATHWAY: Cell wall biogenesis; cell wall polysaccharide biosynthesis.
CC {ECO:0000305|PubMed:18055597}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P9WMX3}.
CC Secreted, cell wall {ECO:0000250|UniProtKB:P9WMX3}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AFP42625.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CP000480; ABK69689.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP42625.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_011731236.1; NZ_SIJM01000013.1.
DR RefSeq; YP_890580.1; NC_008596.1.
DR AlphaFoldDB; A0R5Z2; -.
DR SMR; A0R5Z2; -.
DR STRING; 246196.MSMEI_6199; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR PRIDE; A0R5Z2; -.
DR EnsemblBacteria; ABK69689; ABK69689; MSMEG_6367.
DR EnsemblBacteria; AFP42625; AFP42625; MSMEI_6199.
DR GeneID; 66737645; -.
DR KEGG; msg:MSMEI_6199; -.
DR KEGG; msm:MSMEG_6367; -.
DR PATRIC; fig|246196.19.peg.6195; -.
DR eggNOG; COG1216; Bacteria.
DR OMA; RFYTYRN; -.
DR OrthoDB; 865276at2; -.
DR UniPathway; UPA00963; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016757; F:glycosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0016740; F:transferase activity; IDA:UniProtKB.
DR GO; GO:0045227; P:capsule polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0044038; P:cell wall macromolecule biosynthetic process; IDA:UniProtKB.
DR GO; GO:0071555; P:cell wall organization; IDA:UniProtKB.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cell wall; Cell wall biogenesis/degradation;
KW Glycosyltransferase; Membrane; Reference proteome; Secreted; Transferase.
FT CHAIN 1..302
FT /note="Galactofuranosyltransferase GlfT1"
FT /id="PRO_0000395357"
SQ SEQUENCE 302 AA; 34041 MW; 407E1D4CA50DA4BD CRC64;
MTHTEVVCAV VVTHRRRELL ATSLDAVVSQ DRKPDHLIVV DNDNDPQVRE LVTGQPVPST
YLGSRRNLGG AGGFALGMLH ALALGADWIW LADDDGRPAD TTVLSTLLSC AHTHSLAEVS
PMVCNLDDPQ RLAFPLRRGL VWRRLTSELR TDSSSSSGDL LPGIASLFNG ALFRADTVDA
VGVPDLRLFV RGDEVELHRR LVRSGLPFGT CLTASYLHPC GTDEFKPILG GRMHTQYPDD
ETKRFFTYRN RGYLLSQPGL RKLLPQEWLR FGWYFLVSRR DLAGLREWIR LRRLGRRERF
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