ID   GLFT1_MYCTO             Reviewed;         304 AA.
AC   P9WMX2; L0TGJ5; Q79F98; Q7D4V6;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Galactofuranosyltransferase GlfT1 {ECO:0000250|UniProtKB:P9WMX3};
DE            Short=GalTr 1 {ECO:0000250|UniProtKB:P9WMX3};
DE            EC=2.4.1.287 {ECO:0000250|UniProtKB:P9WMX3};
DE   AltName: Full=Arabinogalactan galactosyltransferase 1 {ECO:0000250|UniProtKB:P9WMX3};
DE   AltName: Full=Rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,4/1,5-galactofuranosyltransferase {ECO:0000250|UniProtKB:P9WMX3};
DE   AltName: Full=UDP-Galf:alpha-3-L-rhamnosyl-alpha-D-GlcNAc-pyrophosphate polyprenol, galactofuranosyl transferase {ECO:0000250|UniProtKB:P9WMX3};
GN   Name=glfT1 {ECO:0000250|UniProtKB:P9WMX3}; OrderedLocusNames=MT3891;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Involved in the biosynthesis of the arabinogalactan (AG)
CC       region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an
CC       essential component of the mycobacterial cell wall. Catalyzes the
CC       transfer of the first two galactofuranosyl (Galf) units from UDP-
CC       galactofuranose (UDP-Galf) onto the rhamnosyl-GlcNAc-diphospho-
CC       decaprenol (Rha-GlcNAc-PP-C50) acceptor, yielding galactofuranosyl-
CC       galactofuranosyl-rhamnosyl-GlcNAc-diphospho-decaprenol (Galf-Galf-Rha-
CC       GlcNAc-PP-C50). Thus, GlfT1 is the initiator of galactan synthesis,
CC       while GlfT2 continues with the subsequent polymerization events.
CC       {ECO:0000250|UniProtKB:P9WMX3}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-L-rhamnosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-
CC         diphospho-trans,octa-cis-decaprenol + 2 UDP-alpha-D-galactofuranose =
CC         beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-
CC         L-rhamnosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-
CC         trans,octa-cis-decaprenol + 2 H(+) + 2 UDP; Xref=Rhea:RHEA:34379,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:66915,
CC         ChEBI:CHEBI:67209, ChEBI:CHEBI:67210; EC=2.4.1.287;
CC         Evidence={ECO:0000250|UniProtKB:P9WMX3};
CC   -!- PATHWAY: Cell wall biogenesis; cell wall polysaccharide biosynthesis.
CC       {ECO:0000250|UniProtKB:P9WMX3}.
CC   -!- SUBUNIT: Is probably part of an AG biosynthetic complex.
CC       {ECO:0000250|UniProtKB:P9WMX3}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P9WMX3}.
CC       Secreted, cell wall {ECO:0000250|UniProtKB:P9WMX3}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK48256.1; -; Genomic_DNA.
DR   PIR; B70696; B70696.
DR   RefSeq; WP_003420606.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WMX2; -.
DR   SMR; P9WMX2; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; AAK48256; AAK48256; MT3891.
DR   KEGG; mtc:MT3891; -.
DR   PATRIC; fig|83331.31.peg.4186; -.
DR   HOGENOM; CLU_023845_2_2_11; -.
DR   UniPathway; UPA00963; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0045227; P:capsule polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cell wall; Cell wall biogenesis/degradation;
KW   Glycosyltransferase; Membrane; Secreted; Transferase.
FT   CHAIN           1..304
FT                   /note="Galactofuranosyltransferase GlfT1"
FT                   /id="PRO_0000427227"
SQ   SEQUENCE   304 AA;  33864 MW;  66AC155D0370FFCA CRC64;
     MTESVFAVVV THRRPDELAK SLDVLTAQTR LPDHLIVVDN DGCGDSPVRE LVAGQPIATT
     YLGSRRNLGG AGGFALGMLH ALAQGADWVW LADDDGHAQD ARVLATLLAC AEKYSLAEVS
     PMVCNIDDPT RLAFPLRRGL VWRRRASELR TEAGQELLPG IASLFNGALF RASTLAAIGV
     PDLRLFIRGD EVEMHRRLIR SGLPFGTCLD AAYLHPCGSD EFKPILCGRM HAQYPDDPGK
     RFFTYRNRGY VLSQPGLRKL LAQEWLRFGW FFLVTRRDPK GLWEWIRLRR LGRREKFGKP
     GGSA