GLFT2_MYCS2
ID GLFT2_MYCS2 Reviewed; 646 AA.
AC A0R628; I7GFR0;
DT 13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Galactofuranosyltransferase GlfT2 {ECO:0000250|UniProtKB:O53585};
DE Short=GalTr 2 {ECO:0000250|UniProtKB:O53585};
DE EC=2.4.1.288 {ECO:0000250|UniProtKB:O53585};
DE AltName: Full=Arabinogalactan galactosyltransferase 2 {ECO:0000250|UniProtKB:O53585};
DE AltName: Full=Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase {ECO:0000250|UniProtKB:O53585};
DE AltName: Full=Polymerizing galactofuranosyltransferase GlfT2 {ECO:0000250|UniProtKB:O53585};
GN Name=glfT2 {ECO:0000250|UniProtKB:O53585};
GN OrderedLocusNames=MSMEG_6403, MSMEI_6235;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
CC -!- FUNCTION: Involved in the galactan polymerization of the
CC arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan
CC (mAGP) complex, an essential component of the mycobacteria cell wall.
CC Thus, successively transfers approximately 28 galactofuranosyl (Galf)
CC residues from UDP-galactofuranose (UDP-Galf) onto the galactofuranosyl-
CC galactofuranosyl-rhamnosyl-GlcNAc-diphospho-decaprenol (Galf-Galf-Rha-
CC GlcNAc-PP-C50) acceptor produced by GlfT1, with alternating 1->5 and
CC 1->6 links, forming a galactan domain with approximately 30
CC galactofuranosyl residues. {ECO:0000250|UniProtKB:O53585}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-
CC alpha-L-rhamnosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-
CC trans,octa-cis-decaprenol + 28 UDP-alpha-D-galactofuranose = [beta-D-
CC galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-
CC galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-
CC rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-
CC trans,octa-cis-decaprenol + 28 H(+) + 28 UDP; Xref=Rhea:RHEA:34391,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:66915,
CC ChEBI:CHEBI:67210, ChEBI:CHEBI:67212; EC=2.4.1.288;
CC Evidence={ECO:0000250|UniProtKB:O53585};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000250|UniProtKB:O53585};
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:O53585};
CC -!- PATHWAY: Cell wall biogenesis; cell wall polysaccharide biosynthesis.
CC {ECO:0000250|UniProtKB:O53585}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:O53585}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:O53585}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR EMBL; CP000480; ABK72427.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP42661.1; -; Genomic_DNA.
DR RefSeq; WP_011731265.1; NZ_SIJM01000013.1.
DR RefSeq; YP_890616.1; NC_008596.1.
DR AlphaFoldDB; A0R628; -.
DR SMR; A0R628; -.
DR STRING; 246196.MSMEI_6235; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR PRIDE; A0R628; -.
DR EnsemblBacteria; ABK72427; ABK72427; MSMEG_6403.
DR EnsemblBacteria; AFP42661; AFP42661; MSMEI_6235.
DR GeneID; 66737680; -.
DR KEGG; msg:MSMEI_6235; -.
DR KEGG; msm:MSMEG_6403; -.
DR PATRIC; fig|246196.19.peg.6229; -.
DR eggNOG; COG1216; Bacteria.
DR OMA; NGWWMCL; -.
DR OrthoDB; 865276at2; -.
DR UniPathway; UPA00963; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016757; F:glycosyltransferase activity; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016740; F:transferase activity; ISS:UniProtKB.
DR GO; GO:0045227; P:capsule polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0044038; P:cell wall macromolecule biosynthetic process; ISS:UniProtKB.
DR GO; GO:0071555; P:cell wall organization; ISS:UniProtKB.
DR InterPro; IPR045699; GlfT2_C.
DR InterPro; IPR040492; GlfT2_N.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF19320; GlfT2_domain3; 1.
DR Pfam; PF17994; Glft2_N; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 3: Inferred from homology;
KW Cell membrane; Cell wall biogenesis/degradation; Glycosyltransferase;
KW Magnesium; Manganese; Membrane; Metal-binding; Reference proteome;
KW Transferase.
FT CHAIN 1..646
FT /note="Galactofuranosyltransferase GlfT2"
FT /id="PRO_0000395358"
FT ACT_SITE 384
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:O53585"
FT BINDING 182
FT /ligand="UDP-alpha-D-galactofuranose"
FT /ligand_id="ChEBI:CHEBI:66915"
FT /evidence="ECO:0000250|UniProtKB:O53585"
FT BINDING 211
FT /ligand="UDP-alpha-D-galactofuranose"
FT /ligand_id="ChEBI:CHEBI:66915"
FT /evidence="ECO:0000250|UniProtKB:O53585"
FT BINDING 240
FT /ligand="UDP-alpha-D-galactofuranose"
FT /ligand_id="ChEBI:CHEBI:66915"
FT /evidence="ECO:0000250|UniProtKB:O53585"
FT BINDING 267
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:O53585"
FT BINDING 267
FT /ligand="UDP-alpha-D-galactofuranose"
FT /ligand_id="ChEBI:CHEBI:66915"
FT /evidence="ECO:0000250|UniProtKB:O53585"
FT BINDING 269
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:O53585"
FT BINDING 408
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:O53585"
SQ SEQUENCE 646 AA; 71573 MW; 58E7858070950B59 CRC64;
MSDIPSGALE ADESRAVSLL SRVILPRPGE PLDVRKLYIE ESTTNSRRAH APTRTSLEIG
PESEVSFATY FNAFPASYWR RWSTLDTVVL RVELTGTARV DVYRSKATGA RITVGGAPVA
GEGDGPAAVE FEIDLTPFED GGWIWFDITT NTAVRVHSAG WYAPVPAPGR ANVAVGIPTF
NRPADCVNAL AALTSDPLVD EVISAVIVSD QGTSKAKDHP GFADAAARLG DRLSIHNQPN
LGGSGGYSRV MYEALKNTDC EQILFMDDDI RIEPDSILRA LAMNRFAKSP ILVGGQMLNL
QEPSHLHVMG EVVDRANFMW TAAPNAEYDH DFAKFKLSDA EEPRTKLLHR RVDVDFNGWW
MCMIPRQIAE ELGQPLPLFI KWDDAEYGLR AGEHGYGTVT LPGAAIWHMA WSDKDDAIDW
QAYFHLRNRL VVAALHWDGP VSGLIASHLK ATIKHLLCLE YSTVAIQNKA MEDFLAGPEN
LFSILESAMP DVRKLRSQYP DAVVLPGATS LPPASDMRRK KIAIPVSKPA IAVNLARGVV
HQLRSHDPET HVRPQINVAT QDARWFSLCR VDGVTVTTAD GRGVVYRQRD RAKMFALLRA
SLRQQARLAR KFDRMRKVYR DALPMLTSTQ KWESVLLTET PEKVGR
