GLGA_ECOLI
ID GLGA_ECOLI Reviewed; 477 AA.
AC P0A6U8; P08323; Q2M793;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Glycogen synthase;
DE EC=2.4.1.21;
DE AltName: Full=Starch [bacterial glycogen] synthase;
GN Name=glgA; OrderedLocusNames=b3429, JW3392;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3097003; DOI=10.1016/s0021-9258(18)66708-0;
RA Kumar A., Larsen C.E., Preiss J.;
RT "Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli
RT ADP-glucose:alpha-1,4-glucan, 4-glucosyltransferase as deduced from the
RT nucleotide sequence of the glgA gene.";
RL J. Biol. Chem. 261:16256-16259(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
RX PubMed=6300111; DOI=10.1016/s0021-9258(18)32541-9;
RA Baecker P.A., Furlong C.E., Preiss J.;
RT "Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli
RT ADP-glucose synthetase as deduced from the nucleotide sequence of the glg C
RT gene.";
RL J. Biol. Chem. 258:5084-5088(1983).
RN [5]
RP PROTEIN SEQUENCE OF 1-10.
RX PubMed=1644773; DOI=10.1128/jb.174.16.5454-5456.1992;
RA Kaidow A., Kataoka T., Wachi M., Takada A., Yamasaki M., Nagai K.;
RT "The 55-kilodalton protein in an oriC complex fraction is glycogen
RT synthase.";
RL J. Bacteriol. 174:5454-5456(1992).
CC -!- FUNCTION: Synthesizes alpha-1,4-glucan chains using ADP-glucose.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1->4)-
CC alpha-D-glucosyl](n+1) + ADP + H(+); Xref=Rhea:RHEA:18189, Rhea:RHEA-
CC COMP:9584, Rhea:RHEA-COMP:9587, ChEBI:CHEBI:15378, ChEBI:CHEBI:15444,
CC ChEBI:CHEBI:57498, ChEBI:CHEBI:456216; EC=2.4.1.21;
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 1 family.
CC Bacterial/plant glycogen synthase subfamily. {ECO:0000305}.
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DR EMBL; V00281; CAA23545.1; -; Genomic_DNA.
DR EMBL; J02616; AAA23870.1; -; Genomic_DNA.
DR EMBL; U18997; AAA58227.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76454.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77863.1; -; Genomic_DNA.
DR EMBL; J01616; AAA98737.1; -; Genomic_DNA.
DR PIR; H65138; SYECGL.
DR RefSeq; NP_417887.1; NC_000913.3.
DR RefSeq; WP_001197646.1; NZ_STEB01000004.1.
DR PDB; 2QZS; X-ray; 2.20 A; A=1-477.
DR PDB; 2R4T; X-ray; 2.26 A; A=1-477.
DR PDB; 2R4U; X-ray; 2.37 A; A=1-477.
DR PDB; 3COP; X-ray; 2.30 A; A=1-477.
DR PDB; 3CX4; X-ray; 2.29 A; A=1-477.
DR PDB; 3D1J; X-ray; 3.00 A; A=1-477.
DR PDB; 3GUH; X-ray; 2.79 A; A=1-477.
DR PDBsum; 2QZS; -.
DR PDBsum; 2R4T; -.
DR PDBsum; 2R4U; -.
DR PDBsum; 3COP; -.
DR PDBsum; 3CX4; -.
DR PDBsum; 3D1J; -.
DR PDBsum; 3GUH; -.
DR AlphaFoldDB; P0A6U8; -.
DR SMR; P0A6U8; -.
DR BioGRID; 4262501; 326.
DR IntAct; P0A6U8; 1.
DR STRING; 511145.b3429; -.
DR jPOST; P0A6U8; -.
DR PaxDb; P0A6U8; -.
DR PRIDE; P0A6U8; -.
DR EnsemblBacteria; AAC76454; AAC76454; b3429.
DR EnsemblBacteria; BAE77863; BAE77863; BAE77863.
DR GeneID; 66672687; -.
DR GeneID; 947932; -.
DR KEGG; ecj:JW3392; -.
DR KEGG; eco:b3429; -.
DR PATRIC; fig|1411691.4.peg.3299; -.
DR EchoBASE; EB0372; -.
DR eggNOG; COG0297; Bacteria.
DR HOGENOM; CLU_009583_18_2_6; -.
DR InParanoid; P0A6U8; -.
DR OMA; TWCPWYM; -.
DR PhylomeDB; P0A6U8; -.
DR BioCyc; EcoCyc:GLYCOGENSYN-MON; -.
DR BioCyc; MetaCyc:GLYCOGENSYN-MON; -.
DR BRENDA; 2.4.1.21; 2026.
DR UniPathway; UPA00164; -.
DR EvolutionaryTrace; P0A6U8; -.
DR PRO; PR:P0A6U8; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0033201; F:alpha-1,4-glucan synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0004373; F:glycogen (starch) synthase activity; IEA:InterPro.
DR GO; GO:0009011; F:starch synthase activity; IDA:EcoCyc.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:0005978; P:glycogen biosynthetic process; IMP:EcoCyc.
DR HAMAP; MF_00484; Glycogen_synth; 1.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR011835; GS/SS.
DR InterPro; IPR013534; Starch_synth_cat_dom.
DR Pfam; PF08323; Glyco_transf_5; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR TIGRFAMs; TIGR02095; glgA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Glycogen biosynthesis;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..477
FT /note="Glycogen synthase"
FT /id="PRO_0000188611"
FT BINDING 15
FT /ligand="ADP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:57498"
FT CONFLICT 63
FT /note="F -> S (in Ref. 1; AAA23870)"
FT /evidence="ECO:0000305"
FT CONFLICT 102..103
FT /note="AY -> VH (in Ref. 1; AAA23870)"
FT /evidence="ECO:0000305"
FT CONFLICT 245
FT /note="L -> P (in Ref. 1; AAA23870)"
FT /evidence="ECO:0000305"
FT CONFLICT 280
FT /note="L -> S (in Ref. 1; AAA23870)"
FT /evidence="ECO:0000305"
FT CONFLICT 286
FT /note="L -> S (in Ref. 1; AAA23870)"
FT /evidence="ECO:0000305"
FT CONFLICT 309
FT /note="L -> S (in Ref. 1; AAA23870)"
FT /evidence="ECO:0000305"
FT CONFLICT 314
FT /note="L -> S (in Ref. 1; AAA23870)"
FT /evidence="ECO:0000305"
FT CONFLICT 317..318
FT /note="LL -> SS (in Ref. 1; AAA23870)"
FT /evidence="ECO:0000305"
FT CONFLICT 387
FT /note="L -> S (in Ref. 1; AAA23870)"
FT /evidence="ECO:0000305"
FT CONFLICT 419
FT /note="S -> N (in Ref. 1; AAA23870)"
FT /evidence="ECO:0000305"
FT CONFLICT 422
FT /note="V -> I (in Ref. 1; AAA23870)"
FT /evidence="ECO:0000305"
FT CONFLICT 434
FT /note="A -> T (in Ref. 1; AAA23870)"
FT /evidence="ECO:0000305"
FT CONFLICT 444
FT /note="R -> C (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 446
FT /note="S -> P (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 461
FT /note="S -> G (in Ref. 1; AAA23870)"
FT /evidence="ECO:0000305"
FT CONFLICT 476
FT /note="L -> S (in Ref. 1; AAA23870)"
FT /evidence="ECO:0000305"
FT STRAND 2..6
FT /evidence="ECO:0007829|PDB:2QZS"
FT TURN 11..13
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 18..32
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 36..42
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 45..50
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 55..60
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 67..74
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 77..83
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 85..88
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 91..93
FT /evidence="ECO:0007829|PDB:3D1J"
FT STRAND 94..96
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 106..120
FT /evidence="ECO:0007829|PDB:2QZS"
FT TURN 121..123
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 131..136
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 138..140
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 143..149
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 153..161
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 168..170
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 171..175
FT /evidence="ECO:0007829|PDB:2QZS"
FT TURN 176..178
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 181..183
FT /evidence="ECO:0007829|PDB:2QZS"
FT TURN 186..189
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 194..196
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 197..204
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 206..212
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 213..218
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 222..225
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 229..237
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 241..243
FT /evidence="ECO:0007829|PDB:2QZS"
FT TURN 250..252
FT /evidence="ECO:0007829|PDB:2QZS"
FT TURN 255..257
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 261..263
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 270..273
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 274..284
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 290..292
FT /evidence="ECO:0007829|PDB:3CX4"
FT STRAND 294..302
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 303..305
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 307..319
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 323..330
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 332..344
FT /evidence="ECO:0007829|PDB:2QZS"
FT TURN 346..348
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 349..354
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 357..366
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 368..372
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 382..389
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 392..398
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 399..404
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 410..414
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 420..423
FT /evidence="ECO:0007829|PDB:2QZS"
FT STRAND 425..427
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 428..442
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 445..457
FT /evidence="ECO:0007829|PDB:2QZS"
FT HELIX 462..476
FT /evidence="ECO:0007829|PDB:2QZS"
SQ SEQUENCE 477 AA; 52822 MW; 177CF37F8C97CF42 CRC64;
MQVLHVCSEM FPLLKTGGLA DVIGALPAAQ IADGVDARVL LPAFPDIRRG VTDAQVVSRR
DTFAGHITLL FGHYNGVGIY LIDAPHLYDR PGSPYHDTNL FAYTDNVLRF ALLGWVGAEM
ASGLDPFWRP DVVHAHDWHA GLAPAYLAAR GRPAKSVFTV HNLAYQGMFY AHHMNDIQLP
WSFFNIHGLE FNGQISFLKA GLYYADHITA VSPTYAREIT EPQFAYGMEG LLQQRHREGR
LSGVLNGVDE KIWSPETDLL LASRYTRDTL EDKAENKRQL QIAMGLKVDD KVPLFAVVSR
LTSQKGLDLV LEALPGLLEQ GGQLALLGAG DPVLQEGFLA AAAEYPGQVG VQIGYHEAFS
HRIMGGADVI LVPSRFEPCG LTQLYGLKYG TLPLVRRTGG LADTVSDCSL ENLADGVASG
FVFEDSNAWS LLRAIRRAFV LWSRPSLWRF VQRQAMAMDF SWQVAAKSYR ELYYRLK
