ID   GLGA_METSB              Reviewed;         483 AA.
AC   B8EN59;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Glycogen synthase {ECO:0000255|HAMAP-Rule:MF_00484};
DE            EC=2.4.1.21 {ECO:0000255|HAMAP-Rule:MF_00484};
DE   AltName: Full=Starch [bacterial glycogen] synthase {ECO:0000255|HAMAP-Rule:MF_00484};
GN   Name=glgA {ECO:0000255|HAMAP-Rule:MF_00484}; OrderedLocusNames=Msil_0213;
OS   Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB
OS   13906 / BL2).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Beijerinckiaceae; Methylocella.
OX   NCBI_TaxID=395965;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2;
RX   PubMed=20472789; DOI=10.1128/jb.00506-10;
RA   Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B.,
RA   Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.;
RT   "Complete genome sequence of the aerobic facultative methanotroph
RT   Methylocella silvestris BL2.";
RL   J. Bacteriol. 192:3840-3841(2010).
CC   -!- FUNCTION: Synthesizes alpha-1,4-glucan chains using ADP-glucose.
CC       {ECO:0000255|HAMAP-Rule:MF_00484}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[(1->4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1->4)-
CC         alpha-D-glucosyl](n+1) + ADP + H(+); Xref=Rhea:RHEA:18189, Rhea:RHEA-
CC         COMP:9584, Rhea:RHEA-COMP:9587, ChEBI:CHEBI:15378, ChEBI:CHEBI:15444,
CC         ChEBI:CHEBI:57498, ChEBI:CHEBI:456216; EC=2.4.1.21;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00484};
CC   -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00484}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 1 family.
CC       Bacterial/plant glycogen synthase subfamily. {ECO:0000255|HAMAP-
CC       Rule:MF_00484}.
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DR   EMBL; CP001280; ACK49194.1; -; Genomic_DNA.
DR   RefSeq; WP_012589264.1; NC_011666.1.
DR   AlphaFoldDB; B8EN59; -.
DR   SMR; B8EN59; -.
DR   STRING; 395965.Msil_0213; -.
DR   CAZy; GT5; Glycosyltransferase Family 5.
DR   EnsemblBacteria; ACK49194; ACK49194; Msil_0213.
DR   KEGG; msl:Msil_0213; -.
DR   eggNOG; COG0297; Bacteria.
DR   HOGENOM; CLU_009583_18_4_5; -.
DR   OMA; TWCPWYM; -.
DR   OrthoDB; 83240at2; -.
DR   UniPathway; UPA00164; -.
DR   Proteomes; UP000002257; Chromosome.
DR   GO; GO:0033201; F:alpha-1,4-glucan synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004373; F:glycogen (starch) synthase activity; IEA:InterPro.
DR   GO; GO:0009011; F:starch synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00484; Glycogen_synth; 1.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR011835; GS/SS.
DR   InterPro; IPR013534; Starch_synth_cat_dom.
DR   Pfam; PF08323; Glyco_transf_5; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
DR   TIGRFAMs; TIGR02095; glgA; 1.
PE   3: Inferred from homology;
KW   Glycogen biosynthesis; Glycosyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..483
FT                   /note="Glycogen synthase"
FT                   /id="PRO_1000135653"
FT   BINDING         18
FT                   /ligand="ADP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:57498"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00484"
SQ   SEQUENCE   483 AA;  51456 MW;  B317FA4AA79A2033 CRC64;
     MSEISVLAIV SEIFPLIKTG GLADVAGALP AALAGEGIEV ATLVPGYPAV MAQIEYAETV
     IDARPLYGAP ARVLRGSAAG LDLFVLDAPH LYDRPGNPYL LPDGRDFPDN PFRFAALCQT
     GSALGFGAAP GFLPDIVHAH DWQAGLAAAY LHYDGRERPG TVATIHNLAF QGKYPAELLG
     ALGLPPQAFS LEGVEYYGTI GFLKAALALS DRITTVSPTY AAEIRTPEHG MGLDGLLRTR
     AERLIGILNG IDDEVWDPSS DPLITEPFDV ETLARRPANK AALQDKFGLD PDPGALLLGV
     VSRLSWQKGL DLLLANLDLL ERLGVQLAVL GSGEPELVRQ LLEAAEARPG RVGAVIGYDE
     AIAHRIQAGA DAIVIPSRFE PCGLTQLCAL RYGALPIVAR VGGLADTIID ANEMALASGI
     GTGLQFSPPT ADMLGATLER AARLWAEPEI WDELIENGML TDVSWRRPAA LYAKLFRDLV
     RER