GLGB1_XANCP
ID GLGB1_XANCP Reviewed; 724 AA.
AC Q8PE48;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=1,4-alpha-glucan branching enzyme GlgB 1;
DE EC=2.4.1.18;
DE AltName: Full=1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 1;
DE AltName: Full=Alpha-(1->4)-glucan branching enzyme 1;
DE AltName: Full=Glycogen branching enzyme 1;
DE Short=BE 1;
GN Name=glgB1; OrderedLocusNames=XCC0135;
OS Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB
OS 528 / LMG 568 / P 25).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xanthomonas.
OX NCBI_TaxID=190485;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25;
RX PubMed=12024217; DOI=10.1038/417459a;
RA da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R.,
RA Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr.,
RA Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G.,
RA Cannavan F., Cardozo J., Chambergo F., Ciapina L.P., Cicarelli R.M.B.,
RA Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B.,
RA Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F.,
RA Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T.,
RA Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A.,
RA Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J.,
RA Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M.,
RA Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A.,
RA Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A.,
RA Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M.,
RA Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P.;
RT "Comparison of the genomes of two Xanthomonas pathogens with differing host
RT specificities.";
RL Nature 417:459-463(2002).
CC -!- FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages
CC in glycogen by scission of a 1,4-alpha-linked oligosaccharide from
CC growing alpha-1,4-glucan chains and the subsequent attachment of the
CC oligosaccharide to the alpha-1,6 position. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC subfamily. {ECO:0000305}.
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DR EMBL; AE008922; AAM39454.1; -; Genomic_DNA.
DR RefSeq; NP_635530.2; NC_003902.1.
DR AlphaFoldDB; Q8PE48; -.
DR SMR; Q8PE48; -.
DR STRING; 340.xcc-b100_0150; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR EnsemblBacteria; AAM39454; AAM39454; XCC0135.
DR KEGG; xcc:XCC0135; -.
DR PATRIC; fig|190485.4.peg.150; -.
DR eggNOG; COG0296; Bacteria.
DR HOGENOM; CLU_004245_3_2_6; -.
DR OMA; FGMKWMM; -.
DR UniPathway; UPA00164; -.
DR Proteomes; UP000001010; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IBA:GO_Central.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IBA:GO_Central.
DR GO; GO:0005978; P:glycogen biosynthetic process; IBA:GO_Central.
DR CDD; cd02855; E_set_GBE_prok_N; 1.
DR Gene3D; 2.60.40.10; -; 2.
DR Gene3D; 2.60.40.1180; -; 1.
DR HAMAP; MF_00685; GlgB; 1.
DR InterPro; IPR006048; A-amylase/branching_C.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006407; GlgB.
DR InterPro; IPR044143; GlgB_N_E_set_prok.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR43651; PTHR43651; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02806; Alpha-amylase_C; 1.
DR Pfam; PF02922; CBM_48; 1.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
DR TIGRFAMs; TIGR01515; branching_enzym; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Glycogen biosynthesis; Glycogen metabolism;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..724
FT /note="1,4-alpha-glucan branching enzyme GlgB 1"
FT /id="PRO_0000188766"
FT ACT_SITE 403
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 456
FT /note="Proton donor"
FT /evidence="ECO:0000250"
SQ SEQUENCE 724 AA; 80088 MW; 9927812712B93C4F CRC64;
MTNRWDPGVT RALAEARHGD AFAVLGAHPS TNGRLLRTYH PGAEHVTAVL ADGREVPLEA
GPEPGLFAGE LPAEGRYRLR IGWPGGTQDT ADPYAFGPLL SDFDLHLISE GHHLQLADAL
GANAVEVDGV RGTRFAVWAP NASRVAVVGD FNSWDARRHP MRLRHQAGVW ELFVPDVGPG
AHYKYQLRGP HGHELPAKAD PVARRAELAP GTASIVADPT PHQWSDDGWM ATRARRQAHD
APMSIYEIHA GSWLREEGLD LDWDGLADRL IPYVADMGFT HVELMPVTEH PFGGSWGYQP
LGLFAPTARF GSPDGFARFV DRCHREGIGV IVDWVPAHFP TDAHGLAHFD GTALYEHADP
REGFHRDWNT LIYNHGRREV SGFLIASALE FLQRYHVDGL RVDAVASMLY RDYSRNAGEW
VPNIHGGREN YETIAFLRRL NEVVREHAPG AVTIAEESTA WPGVTADVSH GGLGFHYKWN
MGWMHDTLHY IGLDPIYRRY HHGELTFSMV YAYSERFVLP ISHDEVVHGK GSLLGRMPGD
DWQRFANLRA YLGFMFTHPG RKLLFMGCEF GQPTEWNHDA GLPWHLLDDA RHRGVQTLVR
DLNHLYAQYP ALHAHDDDPS GFAWLVGDDA ANSVVAFLRK GKRGDAPVLV VINYTPVVQQ
GYRLGVPQGG LWREVFNSDA GIYGGANLGN GGAVTAEPQS MHGHAQSLPL LLPPLGVIVL
APQG
