ID   GLGB_ASPOR              Reviewed;         689 AA.
AC   Q96VA4; Q2TWN9;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=1,4-alpha-glucan-branching enzyme;
DE            EC=2.4.1.18;
DE   AltName: Full=Glycogen-branching enzyme;
GN   Name=gbeA; ORFNames=AO090010000483;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=12501999; DOI=10.1078/0944-5013-00170;
RA   Sasangka P., Matsuno A., Tanaka A., Akasaka Y., Suyama S., Kano S.,
RA   Miyazaki M., Akao T., Kato M., Kobayashi T., Tsukagoshi N.;
RT   "Structural features of the glycogen branching enzyme encoding genes from
RT   aspergilli.";
RL   Microbiol. Res. 157:337-344(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA   Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA   Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA   Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA   Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA   Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA   Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA   Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA   Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA   Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA   Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA   Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC         primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC   -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AB072730; BAB69770.1; -; mRNA.
DR   EMBL; AP007175; BAE66334.1; -; Genomic_DNA.
DR   RefSeq; XP_001827467.1; XM_001827415.2.
DR   AlphaFoldDB; Q96VA4; -.
DR   SMR; Q96VA4; -.
DR   STRING; 510516.Q96VA4; -.
DR   CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   PRIDE; Q96VA4; -.
DR   EnsemblFungi; BAE66334; BAE66334; AO090010000483.
DR   GeneID; 5999601; -.
DR   KEGG; aor:AO090010000483; -.
DR   VEuPathDB; FungiDB:AO090010000483; -.
DR   HOGENOM; CLU_011131_2_2_1; -.
DR   OMA; FGMKWMM; -.
DR   UniPathway; UPA00164; -.
DR   Proteomes; UP000006564; Chromosome 8.
DR   GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-EC.
DR   GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR   GO; GO:0043169; F:cation binding; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.60.40.10; -; 1.
DR   Gene3D; 2.60.40.1180; -; 1.
DR   InterPro; IPR006048; A-amylase/branching_C.
DR   InterPro; IPR037439; Branching_enzy.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   PANTHER; PTHR43651; PTHR43651; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02806; Alpha-amylase_C; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   PIRSF; PIRSF000463; GlgB; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
PE   2: Evidence at transcript level;
KW   Glycogen biosynthesis; Glycosyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..689
FT                   /note="1,4-alpha-glucan-branching enzyme"
FT                   /id="PRO_0000188778"
FT   ACT_SITE        345
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        400
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   689 AA;  78751 MW;  2B478A5334064432 CRC64;
     MGTSQAVDSS PPDGTGVIQL DPWLEPFRDA LKQRFSFIEG WVKAINETEG GLETFSKGYE
     RFGLNVQSNG DIIYREWAPN AVQAQLVGEF NNWDVTAHPM TKNGFGVWEV TVPAVNGAPA
     IPHDSKIKIS MVIPSGERIY RIPAWIKRVV QDLSVSPTYE AVFWNPPTEK QYKFQYSRPK
     RPESLRIYEA HVGISSPETK VATYKEFTSN MLPRIKYLGY NAIQLMAIME HAYYASFGYQ
     VNNFFAASSR YGTPEDLKEL VDKAHSMGLV VLLDVVHSHA SKNVLDGLNM FDGTDHLYFH
     GGGKGRHELW DSRLFNYGHH EVLRFLLSNL RFWMEEYGFD GFRFDGVTSM LYTHHGIGTG
     FSGGYHEYFG SSVDEEGVMY LTLANEMLHN LYPNCITVAE DVSGMPALCL PHSLGGVGFD
     YRLAMAVPDM YIKLLKEKKD DEWDIGNLSF TLTNRRHGEK TIAYAESHDQ ALVGDKTLMM
     WLCDKEMYTH MSVLTEFTPI IERGMALHKL IRLVTHGLGG EGYLNFEGNE FGHPEWLDFP
     RDGNNNSFWY ARRQLNLTED HLLRYKFLND FDRAMQLTEE KYGWLHSPQA YVSLKNETDK
     VLVFERAGLL WIFNFHPTNS FTDYRVGVEQ SGTYRIVLDT DDPAFGGLNR NLKETRFFTT
     DLSWNGRSNF LQVYIPTRTA LVLALEETL