GLGB_CERS4
ID GLGB_CERS4 Reviewed; 728 AA.
AC Q3J3M6;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=1,4-alpha-glucan branching enzyme GlgB {ECO:0000255|HAMAP-Rule:MF_00685};
DE EC=2.4.1.18 {ECO:0000255|HAMAP-Rule:MF_00685};
DE AltName: Full=1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase {ECO:0000255|HAMAP-Rule:MF_00685};
DE AltName: Full=Alpha-(1->4)-glucan branching enzyme {ECO:0000255|HAMAP-Rule:MF_00685};
DE AltName: Full=Glycogen branching enzyme {ECO:0000255|HAMAP-Rule:MF_00685};
DE Short=BE {ECO:0000255|HAMAP-Rule:MF_00685};
GN Name=glgB {ECO:0000255|HAMAP-Rule:MF_00685}; OrderedLocusNames=RHOS4_10400;
GN ORFNames=RSP_2448;
OS Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG
OS 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (Rhodobacter
OS sphaeroides).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Cereibacter.
OX NCBI_TaxID=272943;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203
RC / NCIMB 8253 / ATH 2.4.1.;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C.,
RA Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.;
RT "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages
CC in glycogen by scission of a 1,4-alpha-linked oligosaccharide from
CC growing alpha-1,4-glucan chains and the subsequent attachment of the
CC oligosaccharide to the alpha-1,6 position. {ECO:0000255|HAMAP-
CC Rule:MF_00685}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00685};
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00685}.
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00685}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00685}.
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DR EMBL; CP000143; ABA78608.1; -; Genomic_DNA.
DR RefSeq; WP_011337498.1; NZ_CP030271.1.
DR RefSeq; YP_352509.1; NC_007493.2.
DR AlphaFoldDB; Q3J3M6; -.
DR SMR; Q3J3M6; -.
DR STRING; 272943.RSP_2448; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR DNASU; 3720044; -.
DR EnsemblBacteria; ABA78608; ABA78608; RSP_2448.
DR KEGG; rsp:RSP_2448; -.
DR PATRIC; fig|272943.9.peg.1366; -.
DR eggNOG; COG0296; Bacteria.
DR OMA; FGMKWMM; -.
DR PhylomeDB; Q3J3M6; -.
DR UniPathway; UPA00164; -.
DR Proteomes; UP000002703; Chromosome 1.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-UniRule.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd02855; E_set_GBE_prok_N; 1.
DR Gene3D; 2.60.40.10; -; 2.
DR Gene3D; 2.60.40.1180; -; 1.
DR HAMAP; MF_00685; GlgB; 1.
DR InterPro; IPR006048; A-amylase/branching_C.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006407; GlgB.
DR InterPro; IPR044143; GlgB_N_E_set_prok.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR43651; PTHR43651; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02806; Alpha-amylase_C; 1.
DR Pfam; PF02922; CBM_48; 1.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF81296; SSF81296; 2.
DR TIGRFAMs; TIGR01515; branching_enzym; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Glycogen biosynthesis; Glycogen metabolism;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..728
FT /note="1,4-alpha-glucan branching enzyme GlgB"
FT /id="PRO_0000260686"
FT ACT_SITE 409
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00685"
FT ACT_SITE 462
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00685"
SQ SEQUENCE 728 AA; 81458 MW; C4B3DDDC265B7161 CRC64;
MNVTSKPGEL VPESDVAAIM RGTHGDPFRV LGMHGGGGAP LSVRVFAPQA AEVAVLGQGG
EVLAPLERIG AEGFFAGTVP GEEKFPYRLR FVSGPHEWEA DDPYRFPEVL GELDEYLLGE
GRHYQLYTRL GAHPAEIEGV QGVSFAVWAP NARRVSVVGA FNAWDGRRHP MRKRIGVGVW
ELFVPGLHTG DLYKYELLGP SGERLPLKSD PLSFAQEAPP ATASVVHGLP EAEWHDAGWM
QERESRQRRD APISIYEVHA GSWRQGLDYD ALAEELSAYV REMGFTHVEF LPISEHPFTG
SWGYQPIGLF APTARFGPPE GFARLVDRLH RDGIGVILDW VPAHFPSDAH GLANFDGTHL
YDHADPRQGF HRDWNTQIYN FGRQEVANFL QASALFWLDR YHVDALRVDA VASMLYLDYS
RNAGEWVPNR HGGRENLEAI DFLRGVNERV RLDHPGCITI AEESTAFPQV SRPVEDGGLG
FGFKWNMGWM HDTLGYFRRD PIHRKHHQND LTFGMVYAYS EDFVLPLSHD EVVHGKGSLI
GQMAGDRWQK FANLRAYFGF MWAHPGKKLL FMGGEFAQER EWNHDASLDW HLLDDPSHAG
MKRLVADLNR EYRKRPALHR MDCDPEGFEW IDAGDSENSV LSFLRKAPGE KPVLAVCNLT
PVVRSDYRIG VPEGGEWREI LNSDAAIYGG SDVGNPGGLQ AEEFSWHGRP ASLRLTLPPL
ATIFVTPA
