GLGB_CRYNB
ID GLGB_CRYNB Reviewed; 682 AA.
AC P0CN83; Q55ZX8; Q5KP87;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 45.
DE RecName: Full=1,4-alpha-glucan-branching enzyme;
DE EC=2.4.1.18;
DE AltName: Full=Glycogen-branching enzyme;
GN Name=GLC3; OrderedLocusNames=CNBA3620;
OS Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=283643;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B-3501A;
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT neoformans.";
RL Science 307:1321-1324(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC subfamily. {ECO:0000305}.
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DR EMBL; AAEY01000002; EAL23246.1; -; Genomic_DNA.
DR RefSeq; XP_777893.1; XM_772800.1.
DR AlphaFoldDB; P0CN83; -.
DR SMR; P0CN83; -.
DR EnsemblFungi; AAW40900; AAW40900; CNA03810.
DR EnsemblFungi; EAL23246; EAL23246; CNBA3620.
DR GeneID; 4933620; -.
DR KEGG; cnb:CNBA3620; -.
DR VEuPathDB; FungiDB:CNBA3620; -.
DR HOGENOM; CLU_011131_2_2_1; -.
DR UniPathway; UPA00164; -.
DR Proteomes; UP000001435; Chromosome 1.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-EC.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 2.60.40.1180; -; 1.
DR InterPro; IPR006048; A-amylase/branching_C.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR43651; PTHR43651; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02806; Alpha-amylase_C; 1.
DR Pfam; PF02922; CBM_48; 1.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
PE 3: Inferred from homology;
KW Glycogen biosynthesis; Glycosyltransferase; Transferase.
FT CHAIN 1..682
FT /note="1,4-alpha-glucan-branching enzyme"
FT /id="PRO_0000410096"
FT ACT_SITE 342
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 397
FT /note="Proton donor"
FT /evidence="ECO:0000250"
SQ SEQUENCE 682 AA; 78344 MW; AD1D30BE01EB496F CRC64;
MTAVSLSDGT AVLKTDPWLE PFSGALRERY AAYQKQRTII EEHEGGLAEF SKGYKSMGFQ
IDKNGGVRYR EWASNATEAR LIGEFNNWSH TANPMTKSPF GVWECYVPPV SPGVCAIPHD
SMVKISMTLP GGESIDRIPT WITRVTQDLN ISPIYDGRFW NPPKEQQYQF KHGHSTRPVE
GLKIYEAHVG ISSPNMRVTT YKEFEVDVLP KIKQLGYNCI QMMAIMEHAY YASFGYQVTN
FFAASSRFGT PEELKSLVDK AHELGLTVLL DVVHSHASKN ILDGINMYDG SDHLYFHEGG
RGRHDQWDSR LFNYGQHEVL RFLLSNLRFW MDIYMFDGFR FDGVTSMMYK HHGIGSGFSG
GYHEYFGDSV DLEAMVYLML ANAMLHETYP HVVTIAEDVS GMPTLCRPVA EGGVGFDYRL
SMAIPDMWIK LLKEYTDDQW EMGQIVHNLT NRRHLEKSVA YAESHDQALV GDKTLAFWLM
DKEMYDFMSD LSPLTPIIDR GLALHKMIRF IVHTLGGEAY LNFEGNEFGH PEWMDFPREG
NGNSFAHARR QFNLVDDKLL RYKYLYEFDV AMNWLEDKYK WLNSPQAYVS LKHEGDKMIV
FERAGLLFIF NFHPTQSFTD YRVGVDTAGE YKVILTSDET RFGGHNRIDM GGRYFTTPME
WNGRKNWLQV YSPSRTVLVL GL