GLGB_DICDI
ID GLGB_DICDI Reviewed; 678 AA.
AC Q555Q9; Q8T189;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=1,4-alpha-glucan-branching enzyme;
DE EC=2.4.1.18;
DE AltName: Full=Glycogen-branching enzyme;
GN Name=glgB; ORFNames=DDB_G0274105;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC subfamily. {ECO:0000305}.
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DR EMBL; AAFI02000012; EAL69945.1; -; Genomic_DNA.
DR RefSeq; XP_644004.1; XM_638912.1.
DR AlphaFoldDB; Q555Q9; -.
DR SMR; Q555Q9; -.
DR STRING; 44689.DDB0214943; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR PaxDb; Q555Q9; -.
DR EnsemblProtists; EAL69945; EAL69945; DDB_G0274105.
DR GeneID; 8619432; -.
DR KEGG; ddi:DDB_G0274105; -.
DR dictyBase; DDB_G0274105; glgB.
DR eggNOG; KOG0470; Eukaryota.
DR HOGENOM; CLU_011131_2_2_1; -.
DR InParanoid; Q555Q9; -.
DR OMA; FGMKWMM; -.
DR PhylomeDB; Q555Q9; -.
DR Reactome; R-DDI-3322077; Glycogen synthesis.
DR UniPathway; UPA00164; -.
DR PRO; PR:Q555Q9; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; ISS:UniProtKB.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IBA:GO_Central.
DR GO; GO:0005978; P:glycogen biosynthetic process; ISS:UniProtKB.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 2.60.40.1180; -; 1.
DR InterPro; IPR006048; A-amylase/branching_C.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR43651; PTHR43651; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02806; Alpha-amylase_C; 1.
DR Pfam; PF02922; CBM_48; 1.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
PE 3: Inferred from homology;
KW Glycogen biosynthesis; Glycosyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..678
FT /note="1,4-alpha-glucan-branching enzyme"
FT /id="PRO_0000327602"
FT ACT_SITE 338
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 391
FT /note="Proton donor"
FT /evidence="ECO:0000250"
SQ SEQUENCE 678 AA; 78891 MW; 878594DD748805D7 CRC64;
MTDYSKATDG TKVIHDDPWL EPYKEVIKRR HNQVKNTIQK LEESEGSLLK FSQGYEYFGF
NVTKDGVNYR EWLPSAHEVY LVGDFNQWNK TSHPLERDNY GRWSIFIPNN SNGECAIPHG
SKIKIYLKLA NGNFDYRIPA WIKRVEQTKE NPVFDGVFWN PSKQYVFKNK SPMKPTELRI
YEAHVGMSSE LPEISTYSKF KDTVLPMVKE LGYNCIQLMA VMEHAYYASF GYQVTNFFAI
SSRFGTPEEL KEMIDKAHEM GLLVFLDVVH SHASKNVLDG LNQLDGTDHH YFHSGGRGNH
ELWDSRLFNY GNWEVMRFLL SNLRFYVDEY HFDGFRFDGV TSMIYTHHGL SPACSYDDYF
GGAVDEDALN YLTLANVMLH TLNPSIVTIA EEVTGLATLC RPFSEGGGDF DYRLAMGIPD
KWIELVKEKK DEDWNMGTIA HMLSNRRYKE KNIAYAESHD QSLVGDKTLA FWLMDKEMYT
NMSVTTEETP IIDRGMSLHK MIRLITSSLG GDGYLNFMGN EFGHPEWVDF PREGNNNSLH
HARRRWDLYR NPLLRYKQLR DFDIAMNKAE QEFRWLSSDF AYISLKHEDD KIIVFERASL
IFIFNFHPSK SFSDYRIGSG VPGKFINVLD SDRKEFGGHV RIGKDNYHYT EDKPWHDRKY
SLLIYIPSRT CLVLKKVD
