GLGB_HAEIN
ID GLGB_HAEIN Reviewed; 730 AA.
AC P45177;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=1,4-alpha-glucan branching enzyme GlgB;
DE EC=2.4.1.18;
DE AltName: Full=1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase;
DE AltName: Full=Alpha-(1->4)-glucan branching enzyme;
DE AltName: Full=Glycogen branching enzyme;
DE Short=BE;
GN Name=glgB; OrderedLocusNames=HI_1357;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages
CC in glycogen by scission of a 1,4-alpha-linked oligosaccharide from
CC growing alpha-1,4-glucan chains and the subsequent attachment of the
CC oligosaccharide to the alpha-1,6 position. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC subfamily. {ECO:0000305}.
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DR EMBL; L42023; AAC23004.1; -; Genomic_DNA.
DR PIR; I64118; I64118.
DR RefSeq; NP_439508.1; NC_000907.1.
DR RefSeq; WP_005694003.1; NC_000907.1.
DR AlphaFoldDB; P45177; -.
DR SMR; P45177; -.
DR STRING; 71421.HI_1357; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR EnsemblBacteria; AAC23004; AAC23004; HI_1357.
DR KEGG; hin:HI_1357; -.
DR PATRIC; fig|71421.8.peg.1410; -.
DR eggNOG; COG0296; Bacteria.
DR HOGENOM; CLU_004245_3_2_6; -.
DR OMA; FGMKWMM; -.
DR PhylomeDB; P45177; -.
DR BioCyc; HINF71421:G1GJ1-1382-MON; -.
DR UniPathway; UPA00164; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IBA:GO_Central.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IBA:GO_Central.
DR GO; GO:0005978; P:glycogen biosynthetic process; IBA:GO_Central.
DR CDD; cd02855; E_set_GBE_prok_N; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 2.60.40.1180; -; 1.
DR HAMAP; MF_00685; GlgB; 1.
DR InterPro; IPR006048; A-amylase/branching_C.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006407; GlgB.
DR InterPro; IPR044143; GlgB_N_E_set_prok.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR43651; PTHR43651; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02806; Alpha-amylase_C; 1.
DR Pfam; PF02922; CBM_48; 1.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF81296; SSF81296; 2.
DR TIGRFAMs; TIGR01515; branching_enzym; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Glycogen biosynthesis; Glycogen metabolism;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..730
FT /note="1,4-alpha-glucan branching enzyme GlgB"
FT /id="PRO_0000188710"
FT ACT_SITE 405
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 458
FT /note="Proton donor"
FT /evidence="ECO:0000250"
SQ SEQUENCE 730 AA; 83820 MW; 5B9575317F53769A CRC64;
MTTAVTQAII DGFFDASNGD PFATLGMHET EQGIEIRTLL PDANRMVVIE RESGKEITEL
DCVDERGFFV GVIPNCRQFF AYQLQVFWGN EAQIIEDPYR FHPMIDDLEQ WLLSEGSMLR
PYEVLGAHFM ECDGVSGVNF RLWAPNARRV SIVGDFNYWD GRRHPMRFHS KSGVWELFLP
KASLGQLYKF ELIDCHGNLR LKADPFAFSS QLRPDTASQV SALPNVVEMT EARKKANQGN
QPISIYEVHL GSWRRNLENN FWLDYDQIAD ELIPYVKEMG FTHIEFLPLS EFPFDGSWGY
QPLGLYSPTS RFGSPEAFRR LVKRAHEAGI NVILDWVPGH FPSDTHGLVA FDGTALYEHE
DPREGYHQDW NTLIYNYGRN EVKNFLSSNA LYWLERFGVD GIRVDAVASM IYRDYSRAEG
EWIPNQYGGR ENLEAIEFLK HTNWKIHSEM AGAISIAEES TSFAGVTHPS ENGGLGFNFK
WNMGWMNDTL AYMKLDPIYR QYHHNKMTFG MVYQYSENFV LPLSHDEVVH GKYSLLGKMP
GDTWQKFANL RAYYGYMWGY PGKKLLFMGN EFAQGREWNY EESLDWFLLD ENIGGGWHKG
VLKLVKDLNQ IYQKNRPLFE LDNSPEGFDW LVVDDAANSV LAFERRSSNG ERIIVVSNFT
PVPRHNYRIG VNVAGKYEEI LNTDSMYYEG SNVGNFGCVA SEQIESHGRE NSISVSIPPL
ATVYLRLKTK
