GLGB_KITAU
ID GLGB_KITAU Reviewed; 764 AA.
AC P52980;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=1,4-alpha-glucan branching enzyme GlgB;
DE EC=2.4.1.18;
DE AltName: Full=1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase;
DE AltName: Full=Alpha-(1->4)-glucan branching enzyme;
DE AltName: Full=Glycogen branching enzyme;
DE Short=BE;
GN Name=glgB;
OS Kitasatospora aureofaciens (Streptomyces aureofaciens).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Kitasatospora.
OX NCBI_TaxID=1894;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 10762 / DSM 40127 / CCM 3239 / JCM 4008 / LMG 5968 / NBRC 12843
RC / NCIMB 8234 / A-377;
RX PubMed=8068720; DOI=10.1016/0304-4165(94)90176-7;
RA Homerova D., Kormanec J.;
RT "Cloning of the putative glycogen branching enzyme gene, glgB, from
RT Streptomyces aureofaciens.";
RL Biochim. Biophys. Acta 1200:334-336(1994).
CC -!- FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages
CC in glycogen by scission of a 1,4-alpha-linked oligosaccharide from
CC growing alpha-1,4-glucan chains and the subsequent attachment of the
CC oligosaccharide to the alpha-1,6 position. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC subfamily. {ECO:0000305}.
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DR EMBL; L11647; AAA67437.1; -; Genomic_DNA.
DR PIR; S47569; S47569.
DR AlphaFoldDB; P52980; -.
DR SMR; P52980; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR UniPathway; UPA00164; -.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-UniRule.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd02855; E_set_GBE_prok_N; 1.
DR Gene3D; 2.60.40.10; -; 2.
DR Gene3D; 2.60.40.1180; -; 1.
DR HAMAP; MF_00685; GlgB; 1.
DR InterPro; IPR006048; A-amylase/branching_C.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006407; GlgB.
DR InterPro; IPR044143; GlgB_N_E_set_prok.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR43651; PTHR43651; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02806; Alpha-amylase_C; 1.
DR Pfam; PF02922; CBM_48; 1.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
DR TIGRFAMs; TIGR01515; branching_enzym; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Glycogen biosynthesis; Glycogen metabolism;
KW Glycosyltransferase; Transferase.
FT CHAIN 1..764
FT /note="1,4-alpha-glucan branching enzyme GlgB"
FT /id="PRO_0000188746"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 440
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 493
FT /note="Proton donor"
FT /evidence="ECO:0000250"
SQ SEQUENCE 764 AA; 85325 MW; 6B45482E4A268ACF CRC64;
MSAARQPSPT VRDKAAPEPA APAAPKGARA PRARRAAPPH GVRPAPALAA EERARLLEGR
HHDPHAVLGA RTQRGGVAFR VLRPYAKAVT VVAKGLRTEL VDEGDGLFSG LLPLTGVPDY
RLLVTYDSDE IEVHDPYRFL PALGELDLHL IGEGRHEELW TALGSQPMEH QGVAGTRFTV
WAPNALGVRV TGDFSYWDAV AYPMRSLGAS GVWELFLPGV AEGALYKYEI TRPDGGRTLR
ADPMARYAEV PPANASIVTA SRYEWQDAEW MARRGALAPH QAPMSVYELH LASWRPGLSY
RQLAEQLPAY VKELGFTHVE LMPVAEHPFG GSWGYQVTGF YAPTSRMGTP DDFRFLVDAL
HRAGIGVIVD WVPAHFPRDD WALAEFDGRP LYEHQDPRRA AHPDWGTLEF DYGRKEVRNF
LVANAVYWCQ EFHVDGLRAD AVASMLYLDY SRDEGDWSPN AHGGREDLDA VALLQEMNAT
VYRRFPGVVT IAEESTAWDG VTRPTDSGGL GFGLKWNMGW MHDTLRYVSK EPVHRKYHHH
DMTFGMVYAF SENFVLPISH DEVVHGKRSL VSKMPGDWWQ QRATHRAYLG FMWAHPGKQL
LFMGQEFAQG SEWSETYGPD WWVLDSSYPA AGDHLGVRSL VRDLNRTYTA SPALWERDSV
PEGFAWVEAD AADDNVFAFL RFARDGSPLL CVSNFSPVVR HGYRIGVPQE VGQWREVLNT
DLEPYGGSGV HHARALRPEP VPAQGRAVSL RMTLPPMATV WLRP