ID   GLGB_KLULA              Reviewed;         719 AA.
AC   Q6CX53;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=1,4-alpha-glucan-branching enzyme;
DE            EC=2.4.1.18;
DE   AltName: Full=Glycogen-branching enzyme;
GN   Name=GLC3; OrderedLocusNames=KLLA0A11176g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC         primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC   -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC       subfamily. {ECO:0000305}.
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DR   EMBL; CR382121; CAH03074.1; -; Genomic_DNA.
DR   RefSeq; XP_451486.1; XM_451486.1.
DR   AlphaFoldDB; Q6CX53; -.
DR   SMR; Q6CX53; -.
DR   STRING; 28985.XP_451486.1; -.
DR   CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   EnsemblFungi; CAH03074; CAH03074; KLLA0_A11176g.
DR   GeneID; 2896528; -.
DR   KEGG; kla:KLLA0_A11176g; -.
DR   eggNOG; KOG0470; Eukaryota.
DR   HOGENOM; CLU_011131_2_2_1; -.
DR   InParanoid; Q6CX53; -.
DR   OMA; FGMKWMM; -.
DR   UniPathway; UPA00164; -.
DR   Proteomes; UP000000598; Chromosome A.
DR   GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-EC.
DR   GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR   GO; GO:0043169; F:cation binding; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.60.40.10; -; 1.
DR   Gene3D; 2.60.40.1180; -; 1.
DR   InterPro; IPR006048; A-amylase/branching_C.
DR   InterPro; IPR037439; Branching_enzy.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   PANTHER; PTHR43651; PTHR43651; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02806; Alpha-amylase_C; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   PIRSF; PIRSF000463; GlgB; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
PE   3: Inferred from homology;
KW   Glycogen biosynthesis; Glycosyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..719
FT                   /note="1,4-alpha-glucan-branching enzyme"
FT                   /id="PRO_0000188784"
FT   ACT_SITE        364
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        425
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   719 AA;  82857 MW;  CE6B5780568A37D9 CRC64;
     MTGDNNDIPE NVRGVVEIDS WLTPFAKVLS ERRYLADEWR YKIEHSTNSE GRDTTLAQFA
     RDAYKSFGLH ADPGSLEIRY KEWAPNAVRA FLIGEFNSWN ENANEMQRDE YGIFTTVVPP
     VVDDSGNKQY GIPHDSKVKV CFQLADGSRI YRLPAWITRA TQPDKETAKQ WGPSYEARFW
     NPPVHYSFKN KRPGFMRDTD SLRIYEAHVG ISTPEPKVGS YKEFTQNVLP RIKDLGYDAI
     QLMAIMEHAY YASFGYQVTN FFAASSRFGT PDELKELVDT AHGMGILVLL DVVHSHASKN
     VTDGLNQFDG SDHQYFHSLA SSRGEHPLWD SRLFNYGHFE VQRFLLANLA FYIDVYQFDG
     FRFDGVTSML YLHHGVGEGG AFSGDYNEYL SSERSFVDHE ALAYLMLAND LVRELLPDNG
     ITIAEDVSGY PTLCMPRHLG GAGFDYRLAM ALPDMWIKLL KESRDEDWEM GHIVHTLVNR
     RHREKVVAYT ESHDQALVGD KTLAFWLMDA AMYTDMTILK ESTPVVDRGI ALHKLIRLIT
     HSLGGEAYLN FEGNEFGHPE WLDFPNVNNG DSYHYARRQF NLVDDPLLRY QYLYQFDKAM
     QTTEKNYKWL NTPQAYVSLK HEVDKVIAFE RNGLLFIFNF HPTESFTDYR IGVNEPGCYR
     IVLNSDKHEY GGWDRIDESQ SKYFTTDLKW NERNNFIQVY IPNRTALVLA LDSRVRNGN