GLGB_PICTO
ID GLGB_PICTO Reviewed; 705 AA.
AC Q6L2Z9;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=1,4-alpha-glucan branching enzyme GlgB {ECO:0000255|HAMAP-Rule:MF_00685};
DE EC=2.4.1.18 {ECO:0000255|HAMAP-Rule:MF_00685};
DE AltName: Full=1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase {ECO:0000255|HAMAP-Rule:MF_00685};
DE AltName: Full=Alpha-(1->4)-glucan branching enzyme {ECO:0000255|HAMAP-Rule:MF_00685};
DE AltName: Full=Glycogen branching enzyme {ECO:0000255|HAMAP-Rule:MF_00685};
DE Short=BE {ECO:0000255|HAMAP-Rule:MF_00685};
GN Name=glgB {ECO:0000255|HAMAP-Rule:MF_00685}; OrderedLocusNames=PTO0067;
OS Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC
OS 100828).
OC Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC Picrophilaceae; Picrophilus.
OX NCBI_TaxID=263820;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828;
RX PubMed=15184674; DOI=10.1073/pnas.0401356101;
RA Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C.,
RA Schepers B., Dock C., Antranikian G., Liebl W.;
RT "Genome sequence of Picrophilus torridus and its implications for life
RT around pH 0.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004).
CC -!- FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages
CC in glycogen by scission of a 1,4-alpha-linked oligosaccharide from
CC growing alpha-1,4-glucan chains and the subsequent attachment of the
CC oligosaccharide to the alpha-1,6 position. {ECO:0000255|HAMAP-
CC Rule:MF_00685}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00685};
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00685}.
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00685}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00685}.
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DR EMBL; AE017261; AAT42652.1; -; Genomic_DNA.
DR RefSeq; WP_011176868.1; NC_005877.1.
DR AlphaFoldDB; Q6L2Z9; -.
DR SMR; Q6L2Z9; -.
DR STRING; 263820.PTO0067; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR EnsemblBacteria; AAT42652; AAT42652; PTO0067.
DR GeneID; 2844042; -.
DR KEGG; pto:PTO0067; -.
DR PATRIC; fig|263820.9.peg.80; -.
DR eggNOG; arCOG02951; Archaea.
DR HOGENOM; CLU_004245_4_0_2; -.
DR OMA; FGMKWMM; -.
DR OrthoDB; 2860at2157; -.
DR UniPathway; UPA00164; -.
DR Proteomes; UP000000438; Chromosome.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-UniRule.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd02855; E_set_GBE_prok_N; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 2.60.40.1180; -; 1.
DR HAMAP; MF_00685; GlgB; 1.
DR InterPro; IPR006048; A-amylase/branching_C.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006407; GlgB.
DR InterPro; IPR044143; GlgB_N_E_set_prok.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR43651; PTHR43651; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02806; Alpha-amylase_C; 1.
DR Pfam; PF02922; CBM_48; 1.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
DR TIGRFAMs; TIGR01515; branching_enzym; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Glycogen biosynthesis; Glycogen metabolism;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..705
FT /note="1,4-alpha-glucan branching enzyme GlgB"
FT /id="PRO_0000188772"
FT ACT_SITE 393
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00685"
FT ACT_SITE 446
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00685"
SQ SEQUENCE 705 AA; 82273 MW; 5816875946D2A5C0 CRC64;
MIKLYNTCMD FKCLNDVECS HPEKILGPHL EDAYIIRAYI PIARAAFILI DNKKYQMIDN
GKVFEYRSDN EINDYKILYI DDSGYEKTID DPYRFRPEIS DYDIYLYGTG RLFEAYKTFG
AHLKTIKDVS GCNFVVWAPS ALSVSVVGNF NHWTPGMHPM INVNDSGIWA LFIPGIKENE
VYKFAIKTKN NEIKMKTDPF AFYTEKRPRT GSIVINDDFH WTDNSFKRSE NALSIYEMHL
GSWKRNNGDY YNYREIADML IDHLKKTGFN CVEIMPVMEH PLDISWGYQV VNYFAPTSRY
GKPDDFKYLV NRLHENNIMV ILDFVPAHFP DDDYGLYMFD GTHLYDYEDP RMGRTPDWGT
NIFDFGRNGV RSFIASAAVF WIDKYHVDGL RFDAVTSMIY LDFGRKPGEW IPNINGGNIN
LEAVSLLKEI NDYIHNKYYN VITVAEESST YPGITSESGL NFNYKWNLGW MHDTLDFFHE
DPLYRKYRIN NLTFSVMYMY SENFILPVSH DEVVYGKGSL YRKMPGNKNE KISNVKLFLS
YMFSYPGKKL LFMGNEFAQK NEWNVLSQLS WNDLDDGKYV MELIHDLNNL YKNDFNFYND
KNFSWIDFND KTNTVISFNR GNAVCIFNFT PVERENYAIG VDYPSRYIEI INTDSKKYNG
GNILNESIYA CKYPMHGRRY SIEIDLPPLA AVIMEPEDLN GSSGN
