GLGB_PSEU2
ID GLGB_PSEU2 Reviewed; 741 AA.
AC Q4ZTJ2;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=1,4-alpha-glucan branching enzyme GlgB {ECO:0000255|HAMAP-Rule:MF_00685};
DE EC=2.4.1.18 {ECO:0000255|HAMAP-Rule:MF_00685};
DE AltName: Full=1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase {ECO:0000255|HAMAP-Rule:MF_00685};
DE AltName: Full=Alpha-(1->4)-glucan branching enzyme {ECO:0000255|HAMAP-Rule:MF_00685};
DE AltName: Full=Glycogen branching enzyme {ECO:0000255|HAMAP-Rule:MF_00685};
DE Short=BE {ECO:0000255|HAMAP-Rule:MF_00685};
GN Name=glgB {ECO:0000255|HAMAP-Rule:MF_00685}; OrderedLocusNames=Psyr_2491;
OS Pseudomonas syringae pv. syringae (strain B728a).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX NCBI_TaxID=205918;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B728a;
RX PubMed=16043691; DOI=10.1073/pnas.0504930102;
RA Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A.,
RA Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S.,
RA Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M.,
RA Kyrpides N.C., Ivanova N., Lindow S.E.;
RT "Comparison of the complete genome sequences of Pseudomonas syringae pv.
RT syringae B728a and pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005).
CC -!- FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages
CC in glycogen by scission of a 1,4-alpha-linked oligosaccharide from
CC growing alpha-1,4-glucan chains and the subsequent attachment of the
CC oligosaccharide to the alpha-1,6 position. {ECO:0000255|HAMAP-
CC Rule:MF_00685}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00685};
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00685}.
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00685}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00685}.
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DR EMBL; CP000075; AAY37530.1; -; Genomic_DNA.
DR RefSeq; WP_011267721.1; NC_007005.1.
DR RefSeq; YP_235568.1; NC_007005.1.
DR AlphaFoldDB; Q4ZTJ2; -.
DR SMR; Q4ZTJ2; -.
DR STRING; 205918.Psyr_2491; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR PRIDE; Q4ZTJ2; -.
DR EnsemblBacteria; AAY37530; AAY37530; Psyr_2491.
DR KEGG; psb:Psyr_2491; -.
DR PATRIC; fig|205918.7.peg.2551; -.
DR eggNOG; COG0296; Bacteria.
DR HOGENOM; CLU_004245_3_2_6; -.
DR OMA; FGMKWMM; -.
DR UniPathway; UPA00164; -.
DR Proteomes; UP000000426; Chromosome.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-UniRule.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd02855; E_set_GBE_prok_N; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 2.60.40.1180; -; 1.
DR HAMAP; MF_00685; GlgB; 1.
DR InterPro; IPR006048; A-amylase/branching_C.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006407; GlgB.
DR InterPro; IPR044143; GlgB_N_E_set_prok.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR43651; PTHR43651; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02806; Alpha-amylase_C; 1.
DR Pfam; PF02922; CBM_48; 1.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF81296; SSF81296; 2.
DR TIGRFAMs; TIGR01515; branching_enzym; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Glycogen biosynthesis; Glycogen metabolism;
KW Glycosyltransferase; Transferase.
FT CHAIN 1..741
FT /note="1,4-alpha-glucan branching enzyme GlgB"
FT /id="PRO_0000260680"
FT ACT_SITE 420
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00685"
FT ACT_SITE 473
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00685"
SQ SEQUENCE 741 AA; 84062 MW; 30571708F8CEEF0F CRC64;
MNAPDKTGTR RRAIPAAVDL DALIRAEHRD PFSILGPHGD GGSGQYVRAY LPAALSVRLL
ARDDGRELGE MEMSEVPGFF VGHLEHPQPY LLKINWAGGE QITEDPYSFG PLLGEMDLYL
FAEGNHRDLS SCLGAQVTSV DGVDGVRFAV WAPNARRVSV VGSFNSWDGR RHPMRMRHPT
GVWEIFVPRL QPGEVYKYEI LGAHGILPLK SDPMALSTTL PPDTASKIAA PLQFEWNDQE
WLQSRAGRHE VTAPLSIYEL HAGSWQMEQV EDNQWRQYNW RELADRLIPY VKELGFTHIE
LMPIMEHPFG GSWGYQLLAQ FAPTARYGSP EDFAFFVDAC HRAEIGVILD WVPAHFPTDT
HGLAQFDGTC LYEYADPKEG FHQDWNTLIY NLGRTEVHGF MLASALHWLK HYHIDGLRVD
AVASMLYRDY SRNAGEWVTN RFGGRENLEA IDFLRHLNDV VALEAPGTMV IAEESTAWPG
VSEPTQQGGL GFNYKWNMGW MHDSLQYMEE DPINRGHHHG KLSFSLVYAW SERFVLPISH
DEVVHGKHSL IDKMPGDRWQ KFANLRAYLS FMWTHPGKKL LFMGCEFGQW REWNHDRELD
WYLMQYAEHV GVKNLVGDLN RLYREEKALH ERDADPAGFQ WLVGDDSANS VFAYLRWSND
GEPLLVVANM TPVPRLDYRL GAPMRGAWTE LLNSDAETYA GSNFGNGGEV MTEAEPAHGM
EDSLVLNLPP LAVLILKPKK D
