GLGB_RHOPA
ID GLGB_RHOPA Reviewed; 716 AA.
AC Q6N3P9;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=1,4-alpha-glucan branching enzyme GlgB {ECO:0000255|HAMAP-Rule:MF_00685};
DE EC=2.4.1.18 {ECO:0000255|HAMAP-Rule:MF_00685};
DE AltName: Full=1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase {ECO:0000255|HAMAP-Rule:MF_00685};
DE AltName: Full=Alpha-(1->4)-glucan branching enzyme {ECO:0000255|HAMAP-Rule:MF_00685};
DE AltName: Full=Glycogen branching enzyme {ECO:0000255|HAMAP-Rule:MF_00685};
DE Short=BE {ECO:0000255|HAMAP-Rule:MF_00685};
GN Name=glgB {ECO:0000255|HAMAP-Rule:MF_00685}; OrderedLocusNames=RPA3644;
OS Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Bradyrhizobiaceae; Rhodopseudomonas.
OX NCBI_TaxID=258594;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-98 / CGA009;
RX PubMed=14704707; DOI=10.1038/nbt923;
RA Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L.,
RA Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R.,
RA Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C.,
RA Harrison F.H., Gibson J., Harwood C.S.;
RT "Complete genome sequence of the metabolically versatile photosynthetic
RT bacterium Rhodopseudomonas palustris.";
RL Nat. Biotechnol. 22:55-61(2004).
CC -!- FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages
CC in glycogen by scission of a 1,4-alpha-linked oligosaccharide from
CC growing alpha-1,4-glucan chains and the subsequent attachment of the
CC oligosaccharide to the alpha-1,6 position. {ECO:0000255|HAMAP-
CC Rule:MF_00685}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00685};
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00685}.
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00685}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00685}.
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DR EMBL; BX572604; CAE29085.1; -; Genomic_DNA.
DR RefSeq; WP_011159183.1; NC_005296.1.
DR AlphaFoldDB; Q6N3P9; -.
DR SMR; Q6N3P9; -.
DR STRING; 258594.RPA3644; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR PRIDE; Q6N3P9; -.
DR EnsemblBacteria; CAE29085; CAE29085; RPA3644.
DR GeneID; 66894749; -.
DR KEGG; rpa:RPA3644; -.
DR eggNOG; COG0296; Bacteria.
DR HOGENOM; CLU_004245_3_2_5; -.
DR OMA; FGMKWMM; -.
DR PhylomeDB; Q6N3P9; -.
DR BioCyc; RPAL258594:TX73_RS18615-MON; -.
DR UniPathway; UPA00164; -.
DR Proteomes; UP000001426; Chromosome.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-UniRule.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd02855; E_set_GBE_prok_N; 1.
DR Gene3D; 2.60.40.10; -; 2.
DR Gene3D; 2.60.40.1180; -; 1.
DR HAMAP; MF_00685; GlgB; 1.
DR InterPro; IPR006048; A-amylase/branching_C.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006407; GlgB.
DR InterPro; IPR044143; GlgB_N_E_set_prok.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR43651; PTHR43651; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02806; Alpha-amylase_C; 1.
DR Pfam; PF02922; CBM_48; 1.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF81296; SSF81296; 2.
DR TIGRFAMs; TIGR01515; branching_enzym; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Glycogen biosynthesis; Glycogen metabolism;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..716
FT /note="1,4-alpha-glucan branching enzyme GlgB"
FT /id="PRO_0000188738"
FT ACT_SITE 399
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00685"
FT ACT_SITE 452
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00685"
SQ SEQUENCE 716 AA; 80566 MW; 8D82E5F72869E90D CRC64;
MTVQLSDEAY AVVEGRHADP FRYLGAHPED GGTMVRVLLP DAVAVEVIGD NGDVAPLDQA
HPAGLFVGPL PGASPRYTLR ARFGDTTVEL QDPYRFPPIL SDFDLYLLGE GTDQRLYDKL
GAHPMELEGV AGVGFVVLAP NARRVSVVGD FNFWNPLRHQ MRVRGSGYWE LFVPGARAGD
HYKFDLAGPN GEQLPQKADP LAFAAELRPK TASIVVDATT LPRPRPAPEN INALSAPMSI
YEVHLGSWRR KEGDQWLTYR ELAEQLPAYV RDMGFTHVEF LPVSEHPFDG SWGYQPTGLY
APTSRFGSPE DFCALVDACH AAGIGVILDW VPGHFPDDPH GLGNFDGTAL YEHANPMQGR
HLDWGTLIYN YGRTEVVNFL TANALFWLER YGIDGLRVDA VASMLYLDYS RPAGGWIPNK
FGGRENIEAI DFIRRFNTEV YGKFPHSTTA AEESTAWPQV SRPIEFGGLG FGYKWNMGWM
HDTLNYISKD PIHRKYHHGQ ILFGLHYAFS ENFILPLSHD EVVHGKRSIL GRMPGDEWQR
FANLRAYYAF MFGHPGKKLL FMGSEFGQER EWSHDRSLDW HLLEYPKYSG IQALLRDLNK
LYRSLPALHQ LDCDPFGFEW LITEDANRNL FAWMRKGNDT RARCLVIVNF SPNVYQDYRV
RVPFPGRWRE VFNSDSAGYG GTNVGNGGEV RTLEGLVPEL SLTIPPLAAI FLTPED
